Advertisement

Immobilized Enzymes and other Materials for the Study of Mammalian Cell Surfaces

  • L. B. WingardJr.

Abstract

The branched chain sugar molecules that extend from the surface of mammalian cells appear to play key roles in the regulation of cell growth and cell differentiation, the immune response, and trans-membrane as well as intercellular communication. These sugar chains, known as glycolipids or glycoproteins, extend from protein or lipid moities that are embedded in the ordered lipid bilayer membrane that encloses the cell. In addition, these lipid or protein moieties are capable of undergoing lateral and rotational diffusion within the bilayer membrane. This relative movement of the membrane components gives rise to changing localized surface concentrations of specific cell surface sugars (1, 2). The purpose of this short chapter is to point out a few of the possibilities for the use of immobilized enzymes and other compounds in the study and modification of cell surface constituents.

Keywords

Sialic Acid Immobilize Enzyme Rotational Diffusion Cyanogen Bromide Aminopropyltriethoxy Silane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Robbins, J. C. & Nicolson, G. L. In “Cancer,” Vol. 4 (F. F. Becker, ed.) Plenum Press, New York, 1975, p. 3.Google Scholar
  2. 2.
    Schlessinger, J., Koppel, D. E., Axelrod, D., Jacobson, K., Webb, W. W. & Elson, E. L. Proc. Nat. Acad. Sci. USA 73: 2409, 1976.CrossRefGoogle Scholar
  3. 3.
    Carney, D. H. & Cunningham, D. D. Nature, 268: 602, 1977.CrossRefGoogle Scholar
  4. 4.
    Parker, T. L., Corfield, A. P., Veh, R. W. & Schauer, R. Hoppe. Seyl. Z. Physiol. Chem. 358: 789, 1977.CrossRefGoogle Scholar
  5. 5.
    Bazarian, E. R. & Wingard Jr., L. B. J. Histochem. Cytochem. 27: 125, 1979.CrossRefGoogle Scholar
  6. 6.
    Cameron, D. J. & Erlanger, B. F. J. Immunol. 116: 1313, 1976.Google Scholar
  7. 7.
    Kinzel, V., Kubler, D., Richards, J. & Stohr, M. Science 192: 487, 1976.CrossRefGoogle Scholar
  8. 8.
    Miron, T., Carter, W. G. & Wilchek, M. J. Solid Phase. Biochem. 1: 225, 1976.Google Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • L. B. WingardJr.
    • 1
  1. 1.University of Pittsburgh Medical SchoolPittsburghUSA

Personalised recommendations