Expression of Rabbit Globin mRNA Injected into Fused HeLa Cells
Introduction into a living cell of an isolated messenger RNA allows to answer many interesting questions concerning its translation as well as its stability (see article by Marbaix and Huez). So far the translation of a messenger RNA, upon its manual injection into somatic cells using glass capillaries, has been demonstrated by means of autoradiography (1) or immunofluorescence (2) of the recipient cells. As these detection methods present some limitations, it would be useful in some cases to use classical biochemical methods like gel electrophoresis (followed by autoradiography) to analyse the translation products of an injected mRNA. This implies that a relatively large amount of labelled proteins has to be extracted from the injected cells. This can, of course, be obtained by increasing the number of injected cells. The injection of a large number of cells (over one thousand) is, however, a tedious work. A better approach is to fuse the cells before injection as recently proposed by Graessmann et al. (3). By this way, giant cells are formed which can be injected very easily. The equivalent of one thousand of individual cells can indeed be injected in a matter of minutes.
KeywordsSomatic Cell Giant Cell Translation Product BeLa Cell Manual Injection
Unable to display preview. Download preview PDF.
- 4).Huez, G. Bruck, C., Portetelle, D. and Cleuter, Y. (1979). Translation of rabbit globin mRNA upon injection into fused HeLa cells. FEBS Lett., in press.Google Scholar