Microinjection of Cellular and Viral mRNAs
The technique of microinjection in cultured cells has been used to critically analyze the biological activities of purified mRNAs. Since microinjected RNA is translated within viable somatic cells, in competition with endogenous mRNAs and in the presence of cellular regulatory factors, its observed behavior is likely to represent the activity of the corresponding molecule in vivo. Furthermore, the biological activities of proteins translated from injected mRNAs can be studied in relation to the living cell. Activities such as induction of DNA synthesis and complementation of genetic mutants have proven particularly useful.
KeywordsVirus Particle Rous Sarcoma Virus Virus Release Viral Envelope Glycoprotein Host Sequence
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- 12).Stacey, D.W. In press. Effect of microinjection upon cultured cells. In: Introduction of Macromolecules into Viable Mammalian Cells. (ed., R. Baserga).Google Scholar
- 19).Stacey, D.W. (1976). Microinjection studies in cultured cells: intracellular behavior of exogenous proteins and polyribosomal mRNA. Thesis: The Rockefeller University.Google Scholar