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Microinjection of Cellular and Viral mRNAs

  • D. W. Stacey
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 31)

Abstract

The technique of microinjection in cultured cells has been used to critically analyze the biological activities of purified mRNAs. Since microinjected RNA is translated within viable somatic cells, in competition with endogenous mRNAs and in the presence of cellular regulatory factors, its observed behavior is likely to represent the activity of the corresponding molecule in vivo. Furthermore, the biological activities of proteins translated from injected mRNAs can be studied in relation to the living cell. Activities such as induction of DNA synthesis and complementation of genetic mutants have proven particularly useful.

Keywords

Virus Particle Rous Sarcoma Virus Virus Release Viral Envelope Glycoprotein Host Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • D. W. Stacey
    • 1
  1. 1.Roche Institute of Molecular BiologyNutleyUSA

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