Continuous Cytotoxic T-Cell Lines
Recently, it was reported that it was possible to maintain normal human T lymphocytes in continuous exponential proliferative culture with the aid of a T-cell growth factor (TCGF) derived from lectin-stimulated peripheral blood mononuclear cells (Morgan et al., 1976; Ruscetti et al., 1977). Subsequently, we (Gillis and Smith, 1977; Gillis et al., 1978a; Baker et al., 1979) and others (Rosenberg et al., 1978; Strausser and Rosenberg, 1978; Nabholz et al., 1978) found that antigen-specific cytolytic T cells could be selected, maintained, and cloned in the presence of TCGF (IL2).* The discovery that such cytolytic T-lymphocyte lines (CTLL) were completely dependent upon TCGF (IL2) for their growth allowed for the development of a rapid, sensitive microassay for this mitogenic factor (Gillis et al., 1978b). This development, in turn, provided the means to perform detailed experimentation directed toward a definition of the biochemical and biological characteristics of TCGF(IL2). The results of these experiments, summarized in this chapter, suggest that the ability to culture functional monoclonal T cells will provide the means leading to an increased understanding of the T-cell immune response, in much the same way that the ability to culture immunoglobulin-secreting plasmacytoma cells has contributed to our understanding of the B-cell immune response.
KeywordsConditioned Medium Mixed Lymphocyte Culture Retard Tumor Growth Murine Spleen Cell Syngeneic Tumor Cell
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- Billingham, R. E., Krohn, P. L., and Medawar, 1951, Effect of cortisone on survival of skin homografts in rabbits, Br. Med. J. 5: 4716.Google Scholar
- Nabholz, M., Engers, H. D., Callovo, D., and North, M., 1978, Cloned T-cell lines with specific cytolytic activity, in: Current Topics in Microbiology and Immunology, Vol. 81 ( F. Melchers, M. Potter, and N. Warner, eds.), p. 176, Springer-Verlag, New York.Google Scholar
- Smith, K. A., Gillis, S., and Baker, P. E., 1979a, The Immunotherapeutic utility of cytotoxic T-lymphocyte lines, Proc. Am. Assoc. Cancer Res. 20: 93.Google Scholar
- Smith, K. A., Gillis, S., and Baker, P. E., 1979b, The role of soluble factors in the regulation of T-cell immune reactivity, in: The Molecular Basis of Immune Cell Function ( J. G. Kaplan, ed.), pp. 223–237, Elsevier-North Holland, Amsterdam.Google Scholar
- Smith, K. A., Baker, P. E., Gillis, S., and Ruscetti, F. W., 1980b, Functional and molecular characteristics of T-cell growth factor, Mol. Immunol. (in press).Google Scholar