Continuous Cytotoxic T-Cell Lines

  • Kendall A. Smith
Part of the Contemporary Topics in Immunobiology book series (CTI, volume 11)


Recently, it was reported that it was possible to maintain normal human T lymphocytes in continuous exponential proliferative culture with the aid of a T-cell growth factor (TCGF) derived from lectin-stimulated peripheral blood mononuclear cells (Morgan et al., 1976; Ruscetti et al., 1977). Subsequently, we (Gillis and Smith, 1977; Gillis et al., 1978a; Baker et al., 1979) and others (Rosenberg et al., 1978; Strausser and Rosenberg, 1978; Nabholz et al., 1978) found that antigen-specific cytolytic T cells could be selected, maintained, and cloned in the presence of TCGF (IL2).* The discovery that such cytolytic T-lymphocyte lines (CTLL) were completely dependent upon TCGF (IL2) for their growth allowed for the development of a rapid, sensitive microassay for this mitogenic factor (Gillis et al., 1978b). This development, in turn, provided the means to perform detailed experimentation directed toward a definition of the biochemical and biological characteristics of TCGF(IL2). The results of these experiments, summarized in this chapter, suggest that the ability to culture functional monoclonal T cells will provide the means leading to an increased understanding of the T-cell immune response, in much the same way that the ability to culture immunoglobulin-secreting plasmacytoma cells has contributed to our understanding of the B-cell immune response.


Conditioned Medium Mixed Lymphocyte Culture Retard Tumor Growth Murine Spleen Cell Syngeneic Tumor Cell 


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Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Kendall A. Smith
    • 1
  1. 1.The Hematology Research Laboratory Department of MedicineDartmouth Medical SchoolHanoverUSA

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