The Calcium-Activated Photoprotein Obelin—Effects of Calcium and Strontium and Its Use in the Measurement of Intracellular Ionized Calcium

  • Anthony K. Campbell
  • Robert L. Dormer


It is now nearly one hundred years since the experiments of Ringer (1) showed that Sr2+ could replace extracellular Ca2+ and allow contraction of frog heart to take place. During the time that has elasped since these experiments were carried out not only has the role of Ca2+ in muscle contraction been well defined (2,3), but there has also been increasing evidence for an important role for Ca2+ in hormone action and secretion (4–6). In order to define the role of Ca2+ in a control process five principal approaches have been adopted, involving five distinct questions (Table 1). The first four of these approaches have provided some indirect evidence of a role for Ca2+ in a particular control process, and in a number of cases it has been shown that Sr2+ can replace Ca2+ and allow the physiological stimulus to take place. However no one has yet been able to directly demonstrate the effect of a hormone on the intracellular concentration of ionized Ca2+ or Sr2+ in small cells.


Single Muscle Fibre Squid Giant Axon Transient Recorder Intracellular Free Calcium Concentration Blue Fluorescent Protein 
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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Anthony K. Campbell
    • 1
  • Robert L. Dormer
    • 1
  1. 1.Department of Medical BiochemistryWelsh National School of MedicineCardiff, WalesUK

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