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Interactions of Divalent Cations Affecting Polyribosomal Profiles from Paramecium—With Particular Reference to the Interactions of Strontium with Calcium, Magnesium, and Manganese

  • A. H. Reisner
  • C. A. Bucholtz

Abstract

The role of monovalent and divalent cations on stabilizing ribosomal material— and to a lesser extent polyribosomal material—in prokaryotes and higher eukaryotes has been the subject of considerable study in the past (see ref. 1 for earlier references). More recently Jackson and Larkins (2) reported on the effect of ionic strength, pH, and the chelation of divalent cations using ethyleneglycol-bis(2-aminoethyl ether) tetraacetic acid (EGTA) when isolating polyribosomes from tobacco leaves. They found that the use of EGTA (which has a high affinity for Ca2+, Cu2+, and Zn2+) significantly increased the yield of polyribosomal material, particularly from expanded leaves, while at the same time increasing the proportion of what appears to be 60 S material at the expense of the 80 S ribosomal zone. Furthermore, by using material from unexpanded leaves, which yields quite reasonable amounts of polyribosomes without the use of EGTA, they could greatly reduce the yield by introducing 25 mM Ca2+, Cu2+, or Zn2+. The overall shape of the profiles, however, i.e., the relative proportions of different classes of polyribosomes to one another, appears to have remained unchanged. Earlier, Larkins and Davies (3) reported that Ca2+ caused degradation of polyribosomes extracted from peas by RNAase activation, but this was not found to occur in tobacco leaves, nor have we found any indication of RNAase activation by Ca2+ in the Paramecium system.

Keywords

Density Gradient Divalent Cation Homogenize Medium Monovalent Cation Sucrose Density Gradient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    A. H. Reisner, C. Bucholtz, and B. S. Chandler, Studies on the polyribosomes of Paramecium. II. Effect of divalent cations, Exp. Cell Res. 93(1), 1–14 (1975).CrossRefGoogle Scholar
  2. 2.
    A. O. Jackson and B. A. Larkins, Influence of ionic strength, pH, chelation of divalent metals on isolation of polyribosomes from tobacco leaves, Plant Physiol. 57, 5–10 (1976).CrossRefGoogle Scholar
  3. 3.
    B. A. Larkins and E. Davies, Polyribosomes from peas: Stimulation of polysome degredation by exogenous and endogenous calcium, Plant Physiol. 52, 655–659 (1973).CrossRefGoogle Scholar
  4. 4.
    S. A. Bogatyreva and A. S. Spirin, Vliianie razlichnykh odno-i dvukhvalentnyl kationov na spetsificheskoe sviazyvanie aminoatsil-tRNK s ribosomnoi 30 S subchastitsei. (Rus), Dokl. Akad. Nauk. SSSR 200, 722–24 (1973).Google Scholar
  5. 5.
    K. Igarashi, K. Sugawara, and S. Hirose, Effects on ribosomes of the substitution of spermidine on divalent cations for magnesium ions, J. Biochem. 77(4), 753–59(1975).Google Scholar
  6. 6.
    A. H. Reisner and C. Bucholtz, Studies on polyribosomes of Paramecium. I. Effect of monovalent ions, Exp. Cell Res. 73, 441–455 (1972).CrossRefGoogle Scholar
  7. 7.
    O. P. Van Diggelen, H. Oostrom, and L. Bosch, Associaton products of native and derived ribosomal subunits of E. coli and their stability during centrifugation. FEBS Lett. 19, 115–120 (1971).CrossRefGoogle Scholar
  8. 8.
    C. Aylmer and A. H. Reisner, Thermally-induced explosive migration of Paramecium, J. Gen. Microbiol. 67, 57–61 (1971).Google Scholar
  9. 9.
    A. H. Reisner, J. Rowe, and H. M. Macindoe, Structural studies on the ribosomes of Paramecium: evidence for a “primitive” animal ribosome, J. Mol. Biol. 32, 587–610 (1968).CrossRefGoogle Scholar
  10. 10.
    A. A. Infante and R. Baierlein, Pressure-induced dissociaton of sedimenting ribosomes: effect on sedimentation patterns, Proc. Natl. Acad. Sci. USA 68, 1780–1785 (1971).CrossRefGoogle Scholar
  11. 11.
    A.A. Infante and M. Krauss, Dissociation of ribosomes induced by centrifugation: evidence for doubting conformational changes in ribosomes, Biochim. Biophys. Acta 246, 81–99 (1971).Google Scholar
  12. 12.
    J. G. Hauge, Pressure induced dissociation of ribosomes during ultracentrifugation, FEBS Lett. 17, 169–172 (1971).CrossRefGoogle Scholar
  13. 13.
    A. H. Reisner and C. Bucholtz, The in vivo effect of dimethyl sulphoxide (DMSO) on protein synthesis and the polyribosome profile in Paramecium, J. Cell Physiol. 90(2), 169–177 (1977).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • A. H. Reisner
    • 1
  • C. A. Bucholtz
    • 1
  1. 1.Genetics Research Laboratories, Division of Animal ProductionCommonwealth Scientific and Industrial Research OrganizationEpping, North RydeAustralia

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