Advertisement

Biological Methods for the Separation of Lymphoid Cells

  • Chris D. Platsoucas
  • Nicholas Catsimpoolas
Part of the Biological Separations book series (BIOSEP)

Abstract

Presently, it has been conclusively established that three major classes of cells are involved in the immune response (Miller and Mitchell, 1969; Katz and Benacerraf, 1972; Good, 1972): T lymphocytes which are thymus-dependent and responsible for the so-called cell-mediated immunity and overall regulation of the immune response; B lymphocytes which are thymus independent and functional in the humoral aspects of immunity; and macrophages (and monocytes) which “process” and present the antigen to T lymphocytes and, therefore, regulate the immune response by rather nonspecific means. These three major classes of lymphoid cells, and especially the T and B lymphocytes, are further subdivided to a large number of functionally distinct subpopulations (Stout and Herzenberg, 1975; Lobo et al., 1975; Murphy et al., 1976; Scher et al., 1976; Press et al., 1976; Cantor and Boyse, 1976; Moretta et al., 1977), so that the overall picture of the individual cell types forming the lymphoid cell system appears very complex. The significance and need for cell separation methods in studies of this complex system is obvious. Experiments designed to identify the functional role of lymphocyte subpopulations require “homogeneous” or “pure” populations of cells. Furthermore, precise functional and structural characterization of cells from lymphoproliferative and myeloproliferative disorders can be accomplished only with relatively homogeneous abnormal cell populations.

Keywords

Human Lymphocyte Complement Receptor Lymphocyte Subpopulation Sheep Erythrocyte Mixed Lymphocyte Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ada, G. L., and Byrd, P., 1969, Specific inactivation of antigen-reactive cells with 125I-labelled antigen, Nature (London) 222: 1291–1292.CrossRefGoogle Scholar
  2. Aiuti, F., Cerottini, J.-C., Coombs, R. R. A., Cooper, M., Dickler, H. B., FrOland, S., Fudenberg, H. H., Greaves, M. F., Grey, H. M., Kunkel, H. G., Natvig, J., Preudhome, J.-L., Rabellino, E., Ritts, R. E., Rowe, D. S., Seligmann, M., Siegal, F. P., Stjemsward, J., Terry, W. D., and Wybran, J., 1974, Special technical report: Identification, enumeration and isolation of B and T lymphocytes from human peripheral blood, Scand. J. Immunol. 3: 521–532.CrossRefGoogle Scholar
  3. Alexander, E., and Henkart, P., 1976, The adherence of human Fc receptor-bearing lymphocytes to antigen-antibody complexes. II. Morphologic alterations induced by the substrate, J. Exp. Med. 143: 329–347.PubMedCrossRefGoogle Scholar
  4. Alter, B. J., Schendel, D. J., Bach, M. L., Bach, F. H., Klein, J., and Stimpfling, J. H., 1971, Cell mediated lympholysis. Importance of serologically defined H-2 regions, J. Exp. Med. 137: 1303–1309.CrossRefGoogle Scholar
  5. Altman, A., Cohen, I. R., and Feldman, M., 1973, Normal T cell receptors for alloantigens, Cell. Immunol. 7: 134–141.PubMedCrossRefGoogle Scholar
  6. Ault, K. A., Griffith, A. L., Platsoucas, C. D., and Catsimpoolas, N., 1976, Partial separation of human blood leukocytes by density gradient electrophoresis. Different mobilities of lymphocytes with IgG, those with IgM and IgD, T lymphocytes and monocytes, J. Immunol. 117: 1406–1908.PubMedGoogle Scholar
  7. Axelsson, B., Kimura, A., Hammarström, S., Wigzell, H., Nilsson, K., and Mellstedt, H., 1978, Helix pomatia A hemagglutinin: Selectivity of binding to lymphocyte surface glycoproteins on T cells and certain B cells, Eur. J. Immunol. 8: 757–769.PubMedCrossRefGoogle Scholar
  8. Bach, J.-F., 1973, Evaluation of T-cells and thymic serum factors in man using the rosette technique, Transplant. Rev. 16: 196–217.PubMedGoogle Scholar
  9. Bach, J.-F., Dormont, J., Dardenne, M., and Bolner, H., 1969, In vitro rosette inhibition by anti-human anti-lymphocyte serum, Transplantation 8: 265–268.PubMedCrossRefGoogle Scholar
  10. Bach, J.-F., Delvieu, F., and Delbane, F., 1970, The rheumatoid rosette: A diagnostic test unifying seropositive and seronegative rheumatoid arthritis, Am. J. Med. 49: 213–222.PubMedCrossRefGoogle Scholar
  11. Bach, F. H., Segall, M., Stouber-Zier, K., Sondel, P. M., and Alter, B. J., 1973, Cell-mediated immunity: Separation of cells involved in recognitive and destructive phases, Science 180: 403–405.PubMedCrossRefGoogle Scholar
  12. Barrett, D., 1978, Tissue distribution of human T cells with complement receptors, Clin. Immunol. Immunopathol. 11: 190–201.PubMedCrossRefGoogle Scholar
  13. Basten, A., Sprent, J., and Miller, J. F. A. P., 1972, Receptor for antibody-antigen complexes used to separate T cells from B cells, Nature (London) (New Biol.) 235: 178–180.Google Scholar
  14. Baxley, G., Bishop, G. B., Cooper, A. G., and Wortis, H. H., 1973, Rosetting of human red blood cells to thymocytes and thymus-derived cells, Clin. Exp. Immunol. 15: 385–392.PubMedGoogle Scholar
  15. Bentwich, Z., Douglas, S. D., Siegal, F. P., and Kunkel, H. G., 1973, Human lymphocyte sheep erythrocyte rosette formation: Some characteristics of the interaction, Clin. Immu-nol. Immunopathol. 1: 511–522.CrossRefGoogle Scholar
  16. Berke, G., and Levey, R. H., 1972, Cellular immunoabsorbants in transplantation immunity, J. Exp. Med. 135: 972–984.PubMedCrossRefGoogle Scholar
  17. Bianco, C., 1976, Methods for the study of macrophage Fc and C3 receptors, in In Vitro Methods in Cell-Mediated and Tumor Immunity ( E. R. Bloom and J. R. David, eds.), pp. 407–415, Academic Press, New York.Google Scholar
  18. Bianco, C., and Nussenzweig, V., 1971, Theta-bearing and complement-receptor lymphocytes are distinct population of cells, Science 173: 154–156.PubMedCrossRefGoogle Scholar
  19. Bianco, C., Patrick, R., and Nussenzweig, V., 1970, A population of lymphocytes bearing a membrane receptor for antigen-antibody complement complexes. I. Separation and characterization, J. Exp. Med. 132: 702–720.PubMedCrossRefGoogle Scholar
  20. Binz, H., Lindenmann, J., and Wigzell, H., 1974, Cell-bound receptors for alloantigens on normal lymphocytes, J. Exp. Med. 139: 877–893.PubMedCrossRefGoogle Scholar
  21. Boldt, D. H., MacDermott, R. P., and Jovolan, E. P., 1975, Interaction of plant lectins with purified human lymphocyte populations: Binding characteristics and kinetics of proliferation, J. Immunol. 114: 1532–1540.PubMedGoogle Scholar
  22. Boyle, W., 1968, An extension of the 51Cr-release assay for the estimation of mouse cytotoxins, Transplantation 6: 761–766.PubMedCrossRefGoogle Scholar
  23. Boyse, E. A., Old, L. J., and Chomonlinkov, I., 1964, Cytotoxic test for demonstration of mouse antibody, Methods Med. Res. 10: 39–47.PubMedGoogle Scholar
  24. Boyum, A., 1968, Separation of leukocytes from blood and bone marrow, Scand. J. Clin. Lab. Invest. 21 (Suppl. 97): 9–21.Google Scholar
  25. Braganza, C. M., Stathopoulos, C., Davies, A. J., Elliott, E. V., and Knebel, R. S., 1975, Lymphocyte-erythrocyte (L.E.) rosettes as indicators of the heterogeneity of lymphocytes in a variety of mammalian species, Cell 4: 103–106.PubMedCrossRefGoogle Scholar
  26. Brain, P., Gordon, J., and Willets, W. A., 1970, Rosette formation by peripheral lymphocytes, Clin. Exp. Immunol. 6: 681–688.PubMedGoogle Scholar
  27. Brody, T., 1970, Identification of two cell populations required for mouse immunocompetence, J. Immunol. 105: 126–135.PubMedGoogle Scholar
  28. Brondz, B. D., 1972, Lymphocyte receptors and mechanisms of in vitro cell-mediated immune reactions, Transplant. Rev. 10: 112–137.PubMedGoogle Scholar
  29. Brondz, B. D., and Snegiröva, A. E., 1971, Interaction of immune lymphocytes with the mixtures of target cells possessing selected specificities of the H-2 immunizing allele, Immunology 20: 457–569.PubMedGoogle Scholar
  30. Brown, C. S., Halpem, H., and Wortis, H. H., 1975, Enhancing rosetting of sheep erythrocytes by human peripheral blood T cells in the presence of Dextran, Clin. Exp. Immunol. 20: 505–513.Google Scholar
  31. Burnet, F. M., 1959, The Clonal Selection Theory of Acquired Immunity, Cambridge University Press, London.Google Scholar
  32. Byrd, P., and Ada, G. L., 1969, An in vitro reaction between labelled flagellin or haemocyanin and lymphocyte-like cells from normal animals, Immunology 17: 503–511.Google Scholar
  33. Cantor, H., and Boyse, E. A., 1975, Function subclasses of T lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of Ag, J. Exp. Med. 141: 1376–1389.PubMedCrossRefGoogle Scholar
  34. Cantor, H., and Boyse, E. A., 1976, Regulation of cellular and humoral immune responses by T-cell subclasses, Cold Spring Harbor Symposium, XLL: 23–32.Google Scholar
  35. Cantor, H., and Boyse, E., 1977, Regulation of the immune response by T cell subclasses, Contemp. Top. Immunobiol. 7: 47–61.PubMedGoogle Scholar
  36. Cantor, H., Shen, F.-W., and Boyse, E. A., 1976, Separation of helper T cells from suppressor T cells expressing different Ly components, J. Exp. Med. 143: 1391–1404.PubMedCrossRefGoogle Scholar
  37. Casali, P., and Perussia, B. M., 1977, C3-reacted Sepharose: A preparative method for separating T and B lymphocytes, Clin. Exp. Immunol. 27: 38–42.PubMedGoogle Scholar
  38. Chapel, H. M., 1973, The effects of papain, thypsin, and phospholipase-A on rosette formation, Transplantation 15: 320–331.PubMedCrossRefGoogle Scholar
  39. Charreire, J., and Bach, J. F., 1974, Self and non-self, Lancet 2: 299–300.PubMedCrossRefGoogle Scholar
  40. Chaves, M. A., and Arranhado, E., 1972, Rosette formation and immunosuppressive agents, Lancet 1: 42–43.PubMedCrossRefGoogle Scholar
  41. Chess, L., and Schlossman, S. F., 1976, Anti-immunoglobulin columns and the separation of T, B and null cells, in In Vitro Methods in Cell Mediated and Tumor Immunity ( B. R. Bloom and J. R. David, eds.), pp. 255–261, Academic Press, New York.Google Scholar
  42. Chess, L., and Schlossman, S. F., 1977, Human lymphocyte subpopulations, Adv. Immunol. 25: 213–241.PubMedCrossRefGoogle Scholar
  43. Chess, L., MacDermott, R. P., and Schlossman, S. F., 1974a, Immunologic functions of isolated human lymphocyte subpopulations. I. Quantitative isolation of human T and B cells and response to mitogens, J. Immunol. 113: 1113–1121.PubMedGoogle Scholar
  44. Chess, L., MacDermott, R. P., and Schlossman, S. F., 1974b, Immunologic functions of isolated human lymphocyte subpopulations. II. Antigen triggering of T and B cells in vitro, J. Immunol. 113: 1222–1227.Google Scholar
  45. Chess, L., MacDermott, R. P., Sonde], P., and Schlossman, S., 1974c, Isolation of cells involved in human cellular hypersensitivity, Prog. Immunol. 3: 125–132.Google Scholar
  46. Chess, L., Evans, R., Humphreys, R. F., Strominger, J. L., and Schlossman, S. F., 1976, Inhibition of antibody-dependent cellular cytotoxicity and immunoglobulin synthesis by an antiserum prepared against a human B-cell Ia-like molecule. J. Exp. Med. 144: 113–122.PubMedCrossRefGoogle Scholar
  47. Chiao, J. W., and Good, R. A., 1976, Studies of the presence of membrane receptors for complement, IgG and the sheep erythrocyte rosetting capacity on the same human lymphocytes, Eur J. Immunol. 6: 157–168.PubMedCrossRefGoogle Scholar
  48. Chiao, J. W., Pantic, V. S., and Good, R. A., 1974, Human peripheral lymphocytes bearing both B-cell complement receptors and T-cell characteristics for sheep erythrocytes detected by a mixed rosette technique, Clin. Exp. Immunol. 18: 483–490.PubMedGoogle Scholar
  49. Chiao, J. W., Pantic, V. S., and Good, R. A., 1975, Human lymphocytes bearing both receptors for complement components and SRBC, Clin. Immunol. Immunopathol. 4: 545–552.PubMedCrossRefGoogle Scholar
  50. Chisholm, P. M., and Ford, W. L., 1978, Selection of antigen-specific cells by adherence to allogeneic cell monolayers: Cytolytic activity, graft-vs-host activity and numbers of adherent and nonadherent cells, Eur. J. Immunol. 8: 438–446.PubMedCrossRefGoogle Scholar
  51. Choi, T. K., Sleight, D. R., and Nisonoff, A., 1974, General method for isolation and recovery of B cells bearing specific receptors, J. Exp. Med. 139: 761–766.PubMedCrossRefGoogle Scholar
  52. Clark, W. R., and Kimura, A. K., 1973, Effect of monolayer fractionation of lymphocytes on graft-vs.-host reactivity, Transplantation 16: 110–119.PubMedCrossRefGoogle Scholar
  53. Cohen, A., and Schlesinger, M., 1970, Absorption of guinea pig serum with agar, Transplantation 10: 130–139.PubMedCrossRefGoogle Scholar
  54. Coombs, R. R. A., Gumer, B. W., Wilson, A. B., Holm, G., and Lindgren, B., 1970, Rosette-formation between human lymphocytes and sheep red cells not involving immunoglobulin receptors, Int. Arch. Allergy Appl. Immunol. 39: 658–672.PubMedCrossRefGoogle Scholar
  55. Cordier, G., Samarut, C., and Revillard, J. P., 1977, Changes of Fcy receptor-related properties induced by interaction of human lymphocytes with insoluble immune complexes, J. Immunol. 119: 1943–1949.PubMedGoogle Scholar
  56. Davidson, W. F., and Parish, C. R., 1975, A procedure for removing red cells and dead cells from lymphoid cell suspensions, J. Immunol. Methods 7: 291–300.PubMedCrossRefGoogle Scholar
  57. Dickler, H. B., and Kunkel, H. G., 1972, Interaction of aggregated gammaglobulin with B-lymphocytes, J. Exp. Med. 136: 191–196.PubMedCrossRefGoogle Scholar
  58. Dickler, H. B., Adkinson, M. F., and Terry, W. D., 1974, Evidence for individual human peripheral blood lymphocytes bearing both B and T cell markers, Nature 247: 213–215.PubMedCrossRefGoogle Scholar
  59. Dierich, M. P., Pellegrino, M. A., Ferrone, S., and Reisfeld, R. A., 1974, Evaluation of C3 receptors on lymphoid cells with different complement sources, J. Immunol. 112: 1766–1772.PubMedGoogle Scholar
  60. Djeu, J., Payne, S., Alford, C., Heim, W., Pomeroy, T., Cohen, M., Oldham, R., and Herberman, R. B., 1977, Detection of decreased proportion of lymphocytes forming rosettes with sheep erythrocytes at 29°C in the blood of cancer patients, Clin. Immunol. Immunopathol. 8: 405–411.PubMedCrossRefGoogle Scholar
  61. Djordjevic, B., and Szybalski, W., 1960, Genetics of human cell lines, J. Exp. Med. 112: 509–521.PubMedCrossRefGoogle Scholar
  62. Dorval, G., Wolsh, K. I., Wigzell, H., 1974, Labeled staphylococcal protein A as an immunological probe in the analysis of cell surface markers, Scand. J. Immunol. 3: 405–414.PubMedCrossRefGoogle Scholar
  63. Dwyer, J. M., 1976, Identifying and enumerating human T and B lymphocytes, Prog. Allergy 21: 178–191.PubMedCrossRefGoogle Scholar
  64. Edelman, G. M., Rutishauser, V., and Millette, C. F., 1971, Cell fractionation and arrangement on fibers, beads, and surfaces, Proc. Natl. Acad. Sci. USA 68: 2153–2158.PubMedCrossRefGoogle Scholar
  65. Ehlenberger, A. G., and Nussenzweig, V., 1976, Identification of cells with complement receptors, in In Vitro Methods in Cell-Mediated and Tumor Immunity ( E. R. Bloom and J. R. David, eds.), pp. 113–121, Academic Press, New York.Google Scholar
  66. Ferrarini, M., Moretta, L., Abrile, R., and Durante, M. L., 1975, Receptors for IgG molecules on human lymphocytes forming spontaneous rosettes with sheep red cells, Eur. J. Immunol. 5: 70–72.PubMedCrossRefGoogle Scholar
  67. Florey, M. J., and Peetoom, F., 1976, Modified E-rosette test for detection of total and active rosette forming lymphocytes, J. Immunol. Methods 13: 201–208.PubMedCrossRefGoogle Scholar
  68. Fournier, C., and Charreire, J., 1978, Activation of a human T cell subpopulation bearing receptors for autologous erythrocytes by concanavalin A, J. Immunol. 121: 771–779.PubMedGoogle Scholar
  69. Froland, S. S., 1972, Binding of sheep erythrocytes to human lymphocytes. A probable marker of T lymphocytes, Scand. J. Immunol. 1: 269–276.PubMedCrossRefGoogle Scholar
  70. Froland, S., and Natvig, J. B., 1973, Identification of three different human lymphocyte populations by surface markers, Transplant. Rev. 16: 114–169.PubMedGoogle Scholar
  71. Froland, S., Wisloff, F., and Michaelson, T. E., 1974, Human lymphocytes with receptors for IgG, a population of cells distinct from T and B lymphocytes, Inter. Arch. Allergy Appl. Immunol. 47: 124–138.CrossRefGoogle Scholar
  72. FrOland, S. S., and Wisloff, F., 1976, A rosette technique for identification of human lymphocytes with Fc receptors, in In Vitro Methods in Cell-Mediated and Tumor Immunity ( B. R. Bloom and J. R. David, eds.), pp. 137–142, Academic Press, New York.Google Scholar
  73. Galih, U., and Schlesinger, M., 1975, Subpopulations of human thymus cells differing in their capacity to form stable E-rosettes and in their immunologic reactivity, J. Immunol. 115: 827–833.Google Scholar
  74. Gelfand, M. C., Shin, M. L., Nagle, R. B., Green, I., and Frank, M. M., 1976, The glomerular complement receptor in immunologically mediated renal glomerular injury, N. Engl. J. Med. 295: 10–14.PubMedCrossRefGoogle Scholar
  75. Ghetie, V., Nilsson, K., and Sjöquist, J., 1974, Density gradient separation of lymphoid cells adhering to protein A containing staphylococci, Proc. Natl. Acad. Sci. USA 71: 4831–4835.PubMedCrossRefGoogle Scholar
  76. Ghetie, V., Stalenheim, G., and Sjöquist, J., 1975, Cell separation by staphylococcal protein A-coated erythrocytes, Scand. J. Immunol. 4: 471–477.PubMedCrossRefGoogle Scholar
  77. Ghetie, V., Mota, G., and Sjöquist, J., 1978, Separation of cells by affinity chromatography on SpA-sepharose 6MB, J. Immunol. Methods 21: 133–141.PubMedCrossRefGoogle Scholar
  78. Gilbertsen, R. B., and Metzgar, R. S., 1976, Human T and B lymphocyte rosette tests: Effect of enzymatic modification of sheep erythrocytes (E) and the specificity of neuraminidasetreated E, Cell. Immunol. 24: 97–106.CrossRefGoogle Scholar
  79. Ginsburg, H., Ax, W., and Berke, G., 1969, Graft reaction in tissue culture by normal rat lymphocytes, Transplant. Proc. 1: 551–582.PubMedGoogle Scholar
  80. Giuliano, V. J., Jasin, H. E., Hurd, E. R., and Ziff, M., 1974, Enumeration of B-lymphocytes in human peripheral blood by a rosette method for the detection of surface-bound immunoglobulin, J. Immunol. 112: 1494–1499.PubMedGoogle Scholar
  81. Gluckman, J. C., and Motnambault, P., 1975, Spontaneous autorosette-forming cells in man: A marker for a subset population of T-lymphocytes? Clin. Exp. Immunol. 22: 302–310.PubMedGoogle Scholar
  82. Gmelig-Meyling, F. H. J., Keoy-Blok, L., and Ballieux, R. E., 1974, Complement-dependent cytolysis of human B lymphocytes with anti-light chain antisera, Eur. J. Immunol. 4: 332–337.PubMedCrossRefGoogle Scholar
  83. Gmelig-Meyling, F., Van Der Ham, M., and Ballieux, R. E., 1976, Binding of IgM by human T lymphocytes, Scand. J. Immunol. 5: 487–493.PubMedCrossRefGoogle Scholar
  84. Golan, D. T., and Borel, Y., 1972, Detection of hapten-binding cells by a highly radioactive 12’I-conjugate, J. Exp. Med. 136: 305–317.PubMedCrossRefGoogle Scholar
  85. Gold, E. R., and Fudenberg, H. H., 1967, Chromic chloride: A coupling reagent for passive hemagglutination reactions, J. Immunol. 99: 859–966.PubMedGoogle Scholar
  86. Goldstein, I. J., Hammarström, S., and Sundbland, G., 1975, Precipitation and carbohydrate-binding specificity studies on wheat germ agglutinin, Biochim. Biophys. Acta 405: 53–65.PubMedGoogle Scholar
  87. Goldstein, P., Svedmyr, E., and Wigzell, H., 1971, Cells mediating specific in vitro cytotox-icity, J. Exp. Med. 134: 1385–1397.CrossRefGoogle Scholar
  88. Good, R. A., 1972, Recent studies on the immunodeficiencies of man, Am. J. Pathol. 69: 489–490.Google Scholar
  89. Greaves, M. F., and Brown, G., 1974, Purification of human T and B lymphocytes. J. Immunol. 112: 420–429.PubMedGoogle Scholar
  90. Griffin, F. M., Bianco, C., and Silverstein, S. C., 1975, Characterization of the macrophage receptor for complement and demonstration of its functional independence from the receptor for the Fc portion of immunoglobulin G, J. Exp. Med. 141: 1269–1282.PubMedCrossRefGoogle Scholar
  91. Gross, R. L., Latty, S., Williams, E. L., and Newbeme, P. M., 1975, Abnormal spontaneous rosette formation and rosette inhibition in lung carcinoma, N. Engl. J. Med. 292: 439–443.CrossRefGoogle Scholar
  92. Gupta, S, and Good, R. A., 1978, Human T cell subsets in health and disease, in Human Lymphocyte Differentiation: Its Application to Human Cancer (C. B. Serrou and C. Rosenfeld, eds.), pp. 367–375, Elsevier North-Holland Biomedical Press, Amsterdam.Google Scholar
  93. Gupta, S., and Grieco, M. H., 1974, E-rosette test, Lancet 2: 954–955.Google Scholar
  94. Gupta, S., and Grieco, M. H., 1975, Rosette formation with mouse erythrocytes-probable marker for human B lymphocytes, Inter. Arch. Allergy Appl. Immunol. 49: 734–742.CrossRefGoogle Scholar
  95. Gupta, S., Good, R. A., and Siegal, F. P., 1976a, Rosette formation with mouse erythrocytes. III. Studies in patients with primary immunodeficiency and lymphoproliferative disorders, Clin. Exp. Immunol. 25: 319–327.PubMedGoogle Scholar
  96. Gupta, S., Ross, G., Good, R. A., and Siegal, F. P., 1976b, Surface markers on human eosinophils, Blood 48: 755–793.PubMedGoogle Scholar
  97. Gupta, S., Fernandes, G., Nair, M., and Good, R. A., 1978, Spontaneous and antibody-dependent cell cytotoxicity by human T cell subpopulation, Proc. Natl. Acad. Sci. USA 75: 5137–5141.PubMedCrossRefGoogle Scholar
  98. Haas, W., 1975, Separation of antigen-specific lymphocytes. II. Enrichment of hapten-specific antibody-forming cell precursor, J. Exp. Med. 141: 1015–1029.PubMedCrossRefGoogle Scholar
  99. Haas, W., and Layton, J. E., 1975, Separation of antigen-specific lymphocytes. I. Enrichment of antigen-binding cells, J. Exp. Med. 141: 1004–1014.PubMedCrossRefGoogle Scholar
  100. Haas, W., Schrader, J. W., and Szenberg, A., 1974, A new simple method for the preparation of lymphocytes bearing specific receptors, Eur. J. Immunol. 4: 565–601.PubMedCrossRefGoogle Scholar
  101. Hallberg, T., Burner, B. W., and Coombs, R. R. A., 1973, Opsonic adherence of sensitized ox red cells to human lymphocytes as measured by rosette formation, Inter. Arch. Allergy Appl. Immunol. 44: 500–513.CrossRefGoogle Scholar
  102. Haller, O., Gidlund, M., Hellström, U., Hammarström, S., and Wigzell, H., 1978, A new surface marker on mouse natural killer cells: Receptors for Helix pomatia A hemagglutinin, Eur. J. Immunol. 8: 765–771.PubMedCrossRefGoogle Scholar
  103. Hammarström, S., Hellström, U., Perimann, P., and Dillner, M. L., 1973, A new surface marker on T lymphocytes of human peripheral blood, J. Exp. Med. 138: 1270–1275.PubMedCrossRefGoogle Scholar
  104. Häyry, P., Anderson, L. C., Gahmberg, C., Roberts, P., Ranki, A., Nordling, S., 1975, Fractionation of immunocompetent cells by free-flow cell electrophoresis, Israel J. Med. Sci. 11: 1299–1318.PubMedGoogle Scholar
  105. Heier, H. E., 1974, The influence of mechanical force on the rosette test for human T lymphocytes, Scand. J. Immunol. 3: 677–700.PubMedCrossRefGoogle Scholar
  106. Hoffman, T., and Kunkel, H. G., 1976, The E rosette test, in In Vitro Methods in Cell-Mediated and Tumor Immunity ( B. R. Bloom and J. R. David, eds.), pp. 71–81, Academic Press, New York.Google Scholar
  107. Horowitz, D. A., and Lobo, D. I., 1975, Characterization of two populations of human lymphocytes bearing easily detectable surface immunoglobulin, J. Clin. Invest. 56: 1464–1472.CrossRefGoogle Scholar
  108. Hsu, C. C. S., and Fell, A., 1974, Polymorphonuclear cells form E rosettes, N. Engl. J. Med. 290: 402–403.Google Scholar
  109. Huber, A., Douglas, S. D., and Fudenberg, H. H., 1969, The IgA receptor: An immunological marker for the characterization of mononuclear cells, Immunology 17: 7–21.PubMedGoogle Scholar
  110. Johnson, A. H., Scott, D. W., McKeown, P. T., Pool, P. A., and Amos, D. B., 1977, Affinity chromatography with antifluorescein antibody to separate Ig-positive cells: Preliminary report. Transplant Proc. 9 (Suppl. 1): 145–148.PubMedGoogle Scholar
  111. Johnson, J., 1977, Annual Report of the Basel Institute of Immunology, Communication 1201 Google Scholar
  112. Jondal, M., 1976a, SRBC rosette formation as a human T lymphocyte marker, Scand. J. Immunol. 5 (Suppl. 5): 69–76.PubMedCrossRefGoogle Scholar
  113. Jondal, M., 1976b, Spontaneous lymphocyte-mediated cytotoxicity (SLMC), in In Vitro Methods of Cell-Mediated and Tumor Immunity ( B. R. Bloom and J. R. David, eds.), pp. 263–266, Academic Press, New York.Google Scholar
  114. Jondal, M., Holm, G., and Wigzell, H., 1972, Surface markers on human B and T lymphocytes. I. A large population of lymphocytes forming non-immune rosettes with sheep red blood cells, J. Exp. Med. 136: 207–215.PubMedCrossRefGoogle Scholar
  115. Jondal, M., Wigzell, H., and Aiuti, F., 1973, Human lymphocyte subpopulations. Classification according to surface markers and/or functional characteristics, Transplant. Rev. 16: 163–216.PubMedGoogle Scholar
  116. Julius, M. H., and Herzenberg, L. A., 1974, Isolation of antigen-binding cells from unprimed mice, J. Exp. Med. 140: 904–913.PubMedCrossRefGoogle Scholar
  117. Julius, M. H., Masuda, T., Herzenberg, L. A., 1972, Demonstration that antigen-binding cells are precursors of antibody producing cells after purification with a fluorescence activated cell sorter, Proc. Natl. Acad. Sci. USA 69: 1934–1939.PubMedCrossRefGoogle Scholar
  118. Kaplan, J., 1975, Human T lymphocytes form rosettes with autologous and allogeneic human red blood cells, Clin. Immunol. Immunopathol. 3: 471–475.PubMedCrossRefGoogle Scholar
  119. Kaplan, M. E., and Clark, C. J., 1974, An improved rosetting assay for detection of human T lymphocytes, J. Immunol. Methods 5: 131–137.PubMedCrossRefGoogle Scholar
  120. Kaplan, M. E., Woodson, M., and Clark, C., 1976, Detection of human T lymphocytes by rosette formation with AET-treated sheep red cells, in In Vitro Methods in Cell-Mediated and Tumor Immunity ( B. R. Bloom and J. R. David, eds.), pp. 83–88, Academic Press, New York.Google Scholar
  121. Karpf, M., Gelfand, M. C., Handwenger, B. S., and Schwartz, R. H., 1975, Lack of B lymphocyte depletion from murine spleen cell populations by a human y-globulin column system, J. Immunol. 114: 542–549.PubMedGoogle Scholar
  122. Karsenti, E., Bomens, M. and Avrameas, S., 1975, Stimulation and inhibition of DNA synthesis in rat thymocytes: Action of concanavalin A and wheat germ agglutinin, Eur. J. Immunol. 5: 74–81.PubMedCrossRefGoogle Scholar
  123. Katz, D. H., and Benacerraff, B., 1972, The regulatory influence of activated T cells on B cell responses to antigen, Adv. Immunol. 15: 1–27.PubMedCrossRefGoogle Scholar
  124. Kay, H. D., Bonnard, G. D., West, W. H., Herberman, R. B., 1977, A functional comparison of human Fc-receptor-bearing lymphocytes active in natural cytotoxicity and antibody-dependent cellular cytotoxicity, J. Immunol. 118: 2058–2066.PubMedGoogle Scholar
  125. Kedar, E., Ortiz de Landazuri, M., and Bonavida, B., 1974, Cellular immunoabsorbents: A simplified technique for separation of lymphoid cell populations, J. Immunol. 112: 1231–1243.Google Scholar
  126. Kennedy, J. C. and Axelrad, M. A., 1971, An improved assay for haemolytic plaque-forming cells, Immunology 20: 253–258.PubMedGoogle Scholar
  127. Kotler, R., and Wick, G., 1977, Some methodological aspects of the chromium chloride method for coupling antigen to erythrocytes, J. Immunol. Methods 16: 201–209.CrossRefGoogle Scholar
  128. Kondorosi, E., Nagy, J., and Dénes, G., 1977, Optimal conditions for the separation of rat lymphocytes on anti-immunoglobulin-immunoglobulin affinity columns, J. Immunol. Methods 16: 1–13.PubMedCrossRefGoogle Scholar
  129. Kurnick, J. T., and Grey, H. M., 1975, Relationship between immunoglobulin-bearing lymphocytes and cells reactive with sensitized human erythrocytes, J. Immunol. 115: 305–307.PubMedGoogle Scholar
  130. Lay, W. H., Mendes, N. F., Bianco, C., and Nussenzweig, V., 1971, Binding of sheep red blood cells to a large population of human lymphocytes, Nature (London) 230: 531–532.CrossRefGoogle Scholar
  131. Ling, N. R., Bishop, S., and Jeffries, R., 1977, Use of antibody-coated red cells for the sensitive detection of antigen and in rosette tests for cells bearing surface immunoglobulins, J. Immunol. Methods 15: 279–286.PubMedCrossRefGoogle Scholar
  132. Lobo, P. I., Westervelt, F. B., and Horowitz, D. A., 1975, Identification of two populations of immunoglobulin bearing lymphocytes in man. J. Immunol. 114: 116–121.PubMedGoogle Scholar
  133. Lohrmann, H. P., and Novikous, L., 1974, Rosette formation between T-lymphocytes and unsensitized rhesus monkey erythrocytes, Clin. Immunol. Immunopathol. 3: 99–111.CrossRefGoogle Scholar
  134. Lonai, P., Eliraz, A., Wekerle, H., and Feldman, M., 1973, Depletion of specific graft-versushost reactivity following absorption of nonsensitized lymphocytes on allogeneic fibroblasts, Transplantation 15: 368–393.PubMedCrossRefGoogle Scholar
  135. London, J., Berrich, S., and Bach, J. -F., 1978, Peanut agglutinin—a new tool for studying T lymphocyte subpopulations, J. Immunol. 121: 438–497.PubMedGoogle Scholar
  136. Lotan, R., Skutelsky, E., Damon, D., and Sharon, N., 1975, The purification composition, and specificity of the anti-T lectin from peanut (Arachis hypogaea), J. Biol. Chem. 250: 8518–8529.PubMedGoogle Scholar
  137. MacDermott, R. P., Chess, L., and Schlossman, S. F., 1975, Immunologic functions of iso- lated human lymphocyte subpopulations, Clin. Immunol. Immunopathol. 4: 415–422.CrossRefGoogle Scholar
  138. Mage, M G., and McHugh, L. L., 1973, Retention of graft vs. host activity in non-adherent spleen cells after depletion of cytotoxic activity by incubation on allogeneic target cells, J. Immunol. 111: 652–660.PubMedGoogle Scholar
  139. Mage, M. G., and McHugh, L. L., 1975, Specific partial depletion of graft vs. host activity by incubation and centrifugation of mouse spleen cells on allogeneic spleen cell monolayers, J. Immunol. 115: 911–922.PubMedGoogle Scholar
  140. Mage, M. G., McHugh, L. L., and Rothstein, T. L., 1977, Mouse lymphocytes with and without surface immunoglobulin: Preparative scale separation in polystyrene tissue culture dishes coated with specifically purified anti-immunoglobulin, J. Immunol. Methods 15: 47–56.PubMedCrossRefGoogle Scholar
  141. McArthur, W. P., Chapman, J., and Thorbecke, G. J., 1971, Immunocompetent cells of the chicken, J. Exp. Med. 134: 1036–1047.PubMedCrossRefGoogle Scholar
  142. Mendes, N. F., Tolmai, M. E. A., Selveira, N. P. A., Gilbertson, R. B., and Metzgar, R. S.. 1973, Technical aspects of the rosette tests used to detect human complement receptor (B) and sheep erythrocyte-binding (T) lymphocytes, J. Immunol. 111: 860–868.PubMedGoogle Scholar
  143. Mendes, N. F., Miki, S. S., and Peixinho, F., 1974, Combined detection of human T and B lymphocytes by rosette formation with sheep erythrocytes and Zynosan C3 complexes, J. Immunol. 113: 531–537.PubMedGoogle Scholar
  144. Miller, G. W., Saluk, P. H., and Nussenzweig, V., 1973, Complement-dependent release of immune complexes from the lymphocyte membrane, J. Exp. Med. 138: 495–597.PubMedCrossRefGoogle Scholar
  145. Miller, J. F. A. P., and Mitchell, G. F., 1969, Thymus and antigen-reactive cells, Transplant. Rev. 1: 3–42.PubMedGoogle Scholar
  146. Miller, J. F. A. P., Sprent, J., Basten, A., and Warner, N. L., 1972, Selective cytotoxicity of anti-kappa serum for B lymphocytes, Nature (London) (New Biol.) 237: 18–20.Google Scholar
  147. Moretta, L., Ferrarini, M., Durante, M. L., and Mingari, M. C., 1975, Expression of a recep-tor for IgM by human T cells in vitro, Euro. J. Immunol. 5: 565–570.CrossRefGoogle Scholar
  148. Moretta, L., Ferrarini, M., Mingari, M. C., Moretta, A., and Webb, S. R., 1976a, Subpopulations of human T cells identified by receptor for immunoglobulins and mitogen responsiveness, J. Immunol. 117: 2171–2177.PubMedGoogle Scholar
  149. Moretta, L., Mingari, M. C., and Romanzi, C. A., 19766, Loss of Fc receptors for IgG from human T lymphocytes exposed to IgG immune complexes, Nature (London) 272: 618–620.Google Scholar
  150. Moretta, L., Webb, S. R., Rossi, C. E., Lydyard, P. M., and Cooper, M. D., 1977, Functional analysis of two subpopulations of human T cells and their distribution in immunodeficient patients, J. Exp. Med. 146: 184–197.PubMedCrossRefGoogle Scholar
  151. Moretta, L., Ferrarini, M., and Cooper, M. D., 1978, Characterization of human T-cell sub-populations as defined by specific receptors for immunoglobulin, in Contemporary Topics in Immunobiology ( N. L. Warner and M. D. Cooper, eds.), Volume 8, pp. 19–53, Plenum, New York.CrossRefGoogle Scholar
  152. Murphy, D. B., Herzenberg, L. A., Okumura, K., Herzenberg, L. A., and McDevitt, H. O., 1976, A new I subregion (I-J) marked by a locus (Ia-4) controlling surface determinants on suppressor T-lymphocytes, J. Exp. Med. 144: 699–708.PubMedCrossRefGoogle Scholar
  153. Nagata, Y., and Burger, M. M., 1974, Wheat germ agglutinin. Molecular characteristics and specificity for sugar binding, J. Biol. Chem. 249: 3116–3122.PubMedGoogle Scholar
  154. Naor, E., and Sulitzean, P., 1967, Binding of radioiodinated bovine serum albumin to mouse spleen cells, Nature (London) 214: 687–689.CrossRefGoogle Scholar
  155. Natvig, J. B., and Froland, S. S., 1976, Detection of a third lymphocyte-like cell type by rosette formation with erythrocytes sensitized by various anti-Rh antibodies, Scand. J. Immunol. 5 (Suppl. 5): 83–89.PubMedCrossRefGoogle Scholar
  156. Neefe, J. R., and Sachs, D. H., 1976, Specific elimination of cytotoxic effector cells, J. Exp. Med. 144: 996–1006.PubMedCrossRefGoogle Scholar
  157. Nossal, G. J. V., and Pike, B. L., 1976, Single cell studies on the antibody-forming potential of fractionated, hapten-specific B lymphocytes, Immunology 30: 189–191.PubMedGoogle Scholar
  158. Nossal, G. J. V., and Pike, B. L., 1978, Improved procedures for the fractionation and in vitro stimulation of hapten-specific B lymphocytes, J. Immunol. 120: 145–150.PubMedGoogle Scholar
  159. Nossal, G. J. V., Pike, B. L., and Battye, F. L., 1978, Sequential use of hapten-gelatin fractionation and fluorescence-activated cell sorting in the enrichment of hapten-specific B lymphocytes, Eur. J. Immunol. 8: 151–157.PubMedCrossRefGoogle Scholar
  160. Okumura, K., Takemori, T., Tokuhisa, T., and Tada, T., 1977, Specific enrichment of the suppressor T cell bearing I-J determinants. Parallel functional and serological characterizations, J. Exp. Med. 146: 1234–1245.PubMedCrossRefGoogle Scholar
  161. Osoba, D., 1970, Some physical and radiobiological properties of immunologically reactive mouse spleen cells, J. Exp. Med. 132: 368–382.PubMedCrossRefGoogle Scholar
  162. Parish, C. R., 1975, Separation and functional analysis of subpopulations of lymphocytes bearing complement and Fc receptors, Transplant Rev. 25: 98–120.PubMedGoogle Scholar
  163. Parish, C. R., and Hayward, J. A., 1974a, The lymphocyte surface. I. Relation between Fc receptors, C3 receptors and surface immunoglobulin. Proc. R. Soc. London Ser. B 187: 47–63.CrossRefGoogle Scholar
  164. Parish, C. R., and Hayward, J. A., 1974b, The lymphocyte surface. II. Separation of Fc receptor, C3 receptor and surface immunoglobulin-bearing lymphocytes, Proc. R. Soc. London Ser. B 187: 65–81.CrossRefGoogle Scholar
  165. Parish, C. R., and Hayward, J. A., 1974c, The lymphocyte surface. III. Function of Fc receptor, C3 receptor and surface Ig bearing lymphocytes: Identification of a radioresistant B cell, Proc. R. Soc. London Ser. B 187: 379–395.CrossRefGoogle Scholar
  166. Parish, C. R., and McKenzie, I. F. C., 1977, Direct visualization of T lymphocytes bearing Ia antigens controlled by the I-J subregion, J. Exp. Med. 146: 332–362.PubMedCrossRefGoogle Scholar
  167. Parish, C. R., and McKenzie, I. F. C., 1978, A sensitive rosetting method for detecting sub-populations of lymphocytes which react with alloantisera, J. Immunol. Methods 20: 173–183.PubMedCrossRefGoogle Scholar
  168. Parish, C. R., Kirov, S. M., Bowen, N., and Blanden, R. V., 1974, A one-step procedure for separating mouse T and B lymphocytes, Eur. J. Immunol. 4: 808–815.CrossRefGoogle Scholar
  169. Patel, P. C., Menezes, J., Bourkas, A., Dorval, G., Rivard, G. E., Boulay, G., Stanley, P., and Joncas, J. H., 1978, Rosette formation by human lymphoid cells with monkey red blood cells, Ann. Immunol. (Paris) 120c: 449–552.Google Scholar
  170. Pellegrino, M. A., Ferrone, S., Dierich, M. P., and Reisfeld, R. A., 1975a, Enhancement of sheep red blood cell human lymphocyte-rosette formation by the sulthydryl compound 2-amino ethylisothiouronium bromide, Clin. Immunol. Immunopathol. 3: 324–331.PubMedCrossRefGoogle Scholar
  171. Pellegrino, M. A., Ferrone, S., and Theofilopoulos, A. N., 1975b, Rosette formation of human lymphoid cells with monkey red blood cells, J. Immunol. 115: 1065–1071.PubMedGoogle Scholar
  172. Pellegrino, M. A., Ferrone, S., and Theofilopoulos, A. N., 1976, Isolation of human T and B lymphocytes by rosette formation with 2 aminoethylisothiouronium bromide (AET)treated sheep red blood cells and with monkey red blood cells, J. Immunol. Methods 11: 273–279.PubMedCrossRefGoogle Scholar
  173. Perlmann, P., Perlmann, H., and Wigzell, H., 1972, Lymphocyte mediated cytotoxicity in vitro. Induction and inhibition by humoral antibody and nature of effector cells, Transplant. Rev. 13: 91–114.PubMedGoogle Scholar
  174. Perlmann, P., Wigzell, H., Goldstein, P., Laruan, E. W., Larsson, A., O’Toole, C., Perlmann, H., and Svedmyr, E. A. J., 1973, Cell-mediated cytolysis in vitro: Analysis of active lymphocyte subpopulations in different experimental systems, Adv. Biosci. 21: 71–97.Google Scholar
  175. Perlmann, H., Perlman, P., Pape, G. R., and Hallden, G., 1976, Purification, fractionation and assay of antibody-dependent lymphocytic effector K cells in human blood, Scand. J. Immunol. 5 (Suppl. 5): 57–68.PubMedCrossRefGoogle Scholar
  176. Pichler, W. J., and Knapp. W., 1978, Receptors for IgM on human B lymphocytes, Scand. J. Immunol. 1: 105–109.CrossRefGoogle Scholar
  177. Platsoucas, C. D., Good, R. A., and Gupta, S., 1979, Separation of human T lymphocyte subpopulations (Tµ, Ty) by density gradient electrophoresis, Proc. Natl. Acad. Sci. USA 76: 1972–1976.PubMedCrossRefGoogle Scholar
  178. Potash, M. J., and Knopf, P. M., 1978, Differential sensitivity of memory cell subpopulations to anti-immunoglubulin and complement, Eur. J. Immunol. 8: 711–715.PubMedCrossRefGoogle Scholar
  179. Press, J. L., Klinman, R. R., and McDevitt, H. O., 1976, Expression of Ia antigens on haptenspecific B cells, J. Exp. Med. 144: 414–426.PubMedCrossRefGoogle Scholar
  180. Puck, T. T., and Kao, F., 1967, Genetics of somatic mammalian cells. V. Treatment with 5bromodeoxyuridine and usable light for isolation of nutritionally deficient mutants, Proc. Natl. Acad. Sci. USA 58: 1227–1321.PubMedCrossRefGoogle Scholar
  181. Rabellino, E. M., and Metcalf, D., 1975, Receptors for C3 and IgG on macrophage neutrophil and eosinophil colony cells grown in vitro, J. Immunol. 115: 688–727.Google Scholar
  182. Raff, M. C., 1970, Role of thymus-derived lymphocytes in the secondary humoral immune response in mice, Nature (London) 226: 1257–1258.CrossRefGoogle Scholar
  183. Reisner, Y., Linker-Israel, M., and Sharon, N., 1976a, Separation of mouse thymocytes into two subpopulations by the use of peanut lectin, Cell. Immunol. 25: 129–137.PubMedCrossRefGoogle Scholar
  184. Reisner, Y., Ravid, A., and Sharon, N., 1976b, Use of soybean agglutinin for the separation of mouse B and T lymphocytes, Biochem. Biophys. Res. Commun. 72: 1585–1591.PubMedCrossRefGoogle Scholar
  185. Reisner, Y., Hzicoviter, L., Meshrer, A., and Sharon, N., 1978, Hemopoietic stem cell trans-plantation using mouse bone marrow and spleen cells fractionated by lectins, Proc. Natl. Acad. Sci. USA 75: 2933–2937.PubMedCrossRefGoogle Scholar
  186. Roelants, G. E., and Askonas, B. A., 1971, Cell cooperation in antibody induction. The susceptibility of helper cells to specific lethal radioactive antigen, Eur. J. Immunol. 1: 151–159.PubMedCrossRefGoogle Scholar
  187. Ross, G. D., and Polley, M. J., 1975, Specificity of human lymphocyte complement receptors, J. Exp. Med. 141: 1163–1180.PubMedCrossRefGoogle Scholar
  188. Ross, G. D., and Polley, M. J., 1976, Detection of complement-receptor lymphocytes (CRL), in In Vitro Methods in Cell-Mediated and Tumor Immunity ( E. R. Bloom and J. R. David, eds.), pp. 123–136, Academic Press, New York.Google Scholar
  189. Ross, G. D., Polley, M. J., Rabellino, E. M., and Grey, H. M., 1973, Two different complement receptors on human lymphocytes, J. Exp. Med. 138: 798–814.PubMedCrossRefGoogle Scholar
  190. Ross, G. D., Rabellino, E. M., and Polley, M. J., 1976, Mouse leukocyte C3 receptors, Fed. Proc. 35: 254.Google Scholar
  191. Rubin, B., 1975, Studies on the absorbability of graft vs. host reactive lymphocytes, Clin. Exp. Immunol. 20: 513–519.Google Scholar
  192. Ryser, J.-E., and Vassali, P., 1974, Mouse bone marrow lymphocytes and their differentiation, J. Immunol. 113: 719–726.PubMedGoogle Scholar
  193. Sandilands, G. P., Gray, K. G., Cooney, A. E., Browning, J. D., and Anderson, J. R., 1974, Auto-rosette formation by human thymocytes and lymphocytes, Lancet 1: 27–28.PubMedCrossRefGoogle Scholar
  194. Sandrin, M. S., Potter, T. A., Morgan, G. M., and McKenzie, I. F. C., 1978, Detection of mouse alloantibodies by rosetting with protein A-coated sheep red blood cells, Transplantation 26: 126–129.PubMedCrossRefGoogle Scholar
  195. Samarut, C., Drochier, J., and Revillard, J. P., 1976, Distribution of cells binding erythrocyte-antibody (EA) complexes in human lymphoid populations, Scand. J. Immunol. 5: 221–227.PubMedCrossRefGoogle Scholar
  196. Saxon, A., Feldhaus, J., and Robins, R. A., 1976, Single step separation of human T and B cells using AET treated SRBC rosettes, J. Immunol. Methods 12: 285–292.PubMedCrossRefGoogle Scholar
  197. Scher, I., Sharrow, S. O., Wistar, R., Jr., Asofsky, R., and Paul, W. E., 1976, B-lymphocyte heterogeneity: Ontogenetic development and organ distribution of B-lymphocyte populations defined by their density of surface immunoglobulin, J. Exp. Med. 144: 494–509.PubMedCrossRefGoogle Scholar
  198. Schlossman, S. F., and Hudson, L., 1973, Specific purification of lymphocytes populations on a digestible immunoabsorbent, J. Immunol. 110: 313–315.PubMedGoogle Scholar
  199. Scott, D. W., 1976a, Cellular events in tolerance vs. detection, isolation, and fate of lymphoid cells which bind fluorosceinated antigens in vivo, Cell. Immunol. 22: 311–413.CrossRefGoogle Scholar
  200. Scott, D. W., 1976b, Antifluorescein affinity columns isolation and immunocompetence of lymphocytes that bind fluoresceinated antigens in vivo or in vitro, J. Exp. Med. 144: 69–78.CrossRefGoogle Scholar
  201. Seaman, G. V. F., and Uhlenbruck, G., 1%3, The surface of erythrocytes from some animal sources, Arch. Biochem. Biophys. 100: 493–502.Google Scholar
  202. Shortman, K., Williams, N., and Adams, P., 1972, The separation of different cell classes from lymphoid organs. V. Simple procedures for the removal of cell debris, damaged cells and erythroid cells from lymphoid suspensions, J. Immunol. Methods 1: 273–278.PubMedCrossRefGoogle Scholar
  203. Singer, K. H., Johnston, C., Amos, D. B., and Scott, D. W., 1978, Selective depletion and enrichment of alloreactive cytolytic effector lymphocytes using anti-fluorescein affinity columns, Cell Immunol. 36: 75–85.PubMedCrossRefGoogle Scholar
  204. Smith, R. A., Kerman, R., Ezdinli, E., and Stefani, S., 1975, A modified assay for the detection of human adult active rosette forming lymphocytes, J. Immunol. Methods 8: 175–181.PubMedCrossRefGoogle Scholar
  205. Stathopoulos, G., and Elliot, E. V., 1974, Formation of mouse or sheep red blood cell rosettes by lymphocytes from normal and leukemic individuals, Lancet 2: 600–602.CrossRefGoogle Scholar
  206. Steel, C. M., Evans, J., Smith, M. A., 1975, The sheep-cell rosette test on human peripheral blood lymphocytes: An analysis of some variable factors in the technique, Brit. J. Haematol. 28: 245–252.CrossRefGoogle Scholar
  207. Stout, R. D., and Herzenberg, L. A., 1975, The Fc receptor on thymus derived lymphocytes. II. Mitogen responsiveness of T lymphocytes bearing the Fc receptor, J. Exp. Med. 142: 1041–1050.PubMedCrossRefGoogle Scholar
  208. Strelkauskas, A. J., Teodorescu, M., and Dray, S., 1975, Enumeration and isolation of human T and B lymphocytes by rosette formation with antibody-coated erythrocytes, Clin. Exp. Immunol. 22: 62–67.PubMedGoogle Scholar
  209. Strelkauskas, A. J., Schaut, V., Wilson, B. S., Chess, L., and Schlossman, S. F., 1978, Isolation and characterization of naturally occurring subclasses of human peripheral blood T cells with regulatory functions, J. Immunol. 120: 1278–1286.PubMedGoogle Scholar
  210. Stulting, D., and Berke, G., 1973, Nature of lymphocyte-tumor interaction, A general method for cellular immunoabsorption, J. Exp. Med. 137: 932–945.PubMedCrossRefGoogle Scholar
  211. Stulting, R. D., and Berke, G., 1974, Cellular immunoabsorbents. A new approach in transplantation and tumor immunology, Israel J. Med. Sci. 10: 992–1000.Google Scholar
  212. Takahashi, T., Old, L. J., McIntire, R., and Boyse, E. A., 1971, Immunoglobulin and other surface antigens of cells of the immune system, J. Exp. Med. 134: 815–824.PubMedCrossRefGoogle Scholar
  213. Taniguchi, M., and Miller, J. F. A. P., 1977, Enrichment of specific suppressor T cells and characterizations of their surface markers, J. Exp. Med. 146: 1450–1459.PubMedCrossRefGoogle Scholar
  214. Taniguchi, N., Okuda, N., Moriya, N., Miyawaki, T., and Nagaski, T., 1976, Inhibitory effect of sheep erythrocyte fragments on rosette formation of human T lymphocytes with sheep red blood cells, Clin. Exp. Immunol. 24: 370–373.PubMedGoogle Scholar
  215. Targan, S., and Jondal, M., 1978, Monolayer immune complex (MIC) fractionation of Fc receptor bearing human spontaneous killer cells, J. Immunol. Methods 22: 123–129.PubMedCrossRefGoogle Scholar
  216. Theofilopoulos, A. N., Bokisch, V. A., and Dixon, F. J., 1974, Receptor for soluble C3 and Cab on human lymphoblastoid (Raji) cells, J. Exp. Med. 139: 696–704.PubMedCrossRefGoogle Scholar
  217. Tönder, O., Morse, P. A., and Humphrey, L. J., 1974, Similarities of Fc receptors in human malignant tissue and normal lymphoid tissue, J. Immunol. 113: 1162–1169.PubMedGoogle Scholar
  218. Truffa-Bachi, P., and Wofsy, L., 1970, Specific separation of cells on affinity columns, Proc. Natl. Acad. Sci. USA 66: 685–689.PubMedCrossRefGoogle Scholar
  219. Uhlenbruck, G., Seaman, G. V. F., Coombs, R. R. A., 1967, Factors influencing the agglutinability of red cells. III. Physico-chemical studies on ox red cells of different classes of agglutinability, Vox Sang. 12: 420–428.CrossRefGoogle Scholar
  220. Van Boxel, J., and Rosenstreich, D. L., 1974, Binding of aggregated y-globulin to activated T lymphocytes in guinea pig, J. Exp. Med. 139: 1002–1014.PubMedCrossRefGoogle Scholar
  221. von Boehmer, H., and Shortman, K., 1973, The separation of different cell classes from lymphoid organs. IX. A simple and rapid method for removal of damaged cells from lymphoid cell suspensions, J. Immunol. Methods 2: 293–301.CrossRefGoogle Scholar
  222. Wahl, S. J., Everson, G. M., and Oppenheim, J., 1974, Induction of guinea pig B cell lymphokine synthesis by mitogenic and non-mitogenic signals to Fc, Ig, and C3 receptors, J. Exp. Med. 140: 1631–1644.PubMedCrossRefGoogle Scholar
  223. Wahl, S. M., Rosenstreich, D. L., and Oppenheim, J. J., 1976, Separation of human lymphocytes by E rosette sedimentation, in In Vitro Methods in Cell-Mediated and Tumor Immunity ( B. R. Bloom and J. R. David, eds.), pp. 231–240, Academic Press, New York.Google Scholar
  224. Webb, S. R., and Cooper, M. D., 1973, T cells can bind antigen via cytophilic IgM antibody, made by B cells. J. Immnol. 111: 275–281.Google Scholar
  225. Weiner, M. S., Bianco, C., and Nussenzweig, V., 1973, Enhanced binding of neuraminidasetreated sheep erythrocytes to human T lymphocytes, Blood 42: 939–951.PubMedGoogle Scholar
  226. West, W. H., Sienknecht, C. W., Townes, A. S., and Herberman, R. B., 1976, Performance of a rosette assay between lymphocytes and sheep erythrocytes at elevated temperatures to study patients with cancer and other diseases, Clin. Immunol. Immunopathol. 5: 60–66.PubMedCrossRefGoogle Scholar
  227. West, W. H., Cannon, G. B., Kay, H. D., Bonnard, G. D., and Herberman, R. B., 1977a, Natural cytotoxic reactivity of human lymphocytes against a myeloid cell line: Characterization of effector cells, J. Immunol. 118: 355–361.PubMedGoogle Scholar
  228. West, W. H., Payne, S. M., Weese, J. L., and Herberman, R. B., 1977b, Human T lymphocyte subpopulations: Correlation between E-rosette-forming affinity and expression of the Fc receptor, J. Immunol. 119: 548–553.PubMedGoogle Scholar
  229. West, W. H., Boozer, R. B., and Herberman, R. B., 1978, Low affinity E-rosette formation by the human K cell, J. Immunol. 120: 90–95.PubMedGoogle Scholar
  230. Wigzell, H., 1970, Specific fractionation of immunocompetent cells, Transplant. Rev. 5: 76–96.PubMedGoogle Scholar
  231. Wigzell, H., 1971, Cellular immunoabsorbents, in Progress in Immunology (B. Amos, ed.), Academic Press, pp. 1105–1113, New York.Google Scholar
  232. Wigzell, H., 1976a, Enrichment or depletion of surface-immunoglobulin-coated cells using anti-immunoglobulin antibodies and glass or plastic bead columns, in In Vitro Methods in Cell-Mediated and Tumor Immunity ( B. R. Bloom and J. R. David, eds.), pp. 245–253, Academic Press, New York.Google Scholar
  233. Wigzell, H., 1976b, Specific affinity fractionation of lymphocytes using glass or plastic bead columns, Scand. J. Immunol. 5 (Suppl. 5): 23–29.PubMedCrossRefGoogle Scholar
  234. Wigzell, H., and Andersson, B., 1969, Cell separation on antigen coated columns. Elimination of high rate antibody forming cells and immunological memory cells, J. Exp. Med. 129: 23–24.PubMedCrossRefGoogle Scholar
  235. Wigzell, H., and Andersson, B., 1971, Isolation of lymphoid cells with active surface receptor sites, Annu. Rev. Microbiol. 25: 291–308.PubMedCrossRefGoogle Scholar
  236. Wigzell, H., and Mäkelä, O., 1970, Separation of normal and immune lymphoid cells by antigen-coated columns. Antigen-binding characteristics of membrane antibodies as analyzed by hapten-protein antigens, J. Exp. Med. 132: 110–125.PubMedCrossRefGoogle Scholar
  237. Wigzell, H., Sundquist, K. G., and Yoshida, T. O., 1972, Separation of cells according to surface antigens by the use of antibody-coated columns. Fractionation of cells carrying immunoglobulins and blood group antigen, Scand. J. Immunol. 1: 75–81.PubMedCrossRefGoogle Scholar
  238. Wilson, D. B., 1965, Quantitative studies on the behavior of sensitized lymphocytes in vitro, J. Exp. Med. 122: 143–152.PubMedCrossRefGoogle Scholar
  239. Wilson, J. D., 1973, The functions of immune T and B rosette-forming cells. Immunology 25: 185–191.Google Scholar
  240. Winchester, R. J., and Ross, G., 1976, Methods for enumerating lymphocyte population, in Manual of Clinical Immunology ( N. R. Rose and H. Friedman, eds.), pp. 64–76, American Society for Microbiology, Washington D.C.Google Scholar
  241. Winchester, R. J., Fu, S. M., Hoffman, T., and Kunkel, H. G., 1975, IgG on lymphocyte surfaces: Technical problems and the significance of a third cell population, J. Immunol. 114: 1210–1214.PubMedGoogle Scholar
  242. Wofsy, L., Kimura, J., and Truffa-Bachi, P., 1971, Cell separation on affinity columns: The preparation of pure populations of anti-hapten specific lymphocytes, J. Immunol. 107: 725–731.PubMedGoogle Scholar
  243. Wybran, J., and Fudenberg, H. H., 1971, Rosette-formation, a test for cellular immunity. Trans. Assoc. Am. Physicians 84: 239–244.PubMedGoogle Scholar
  244. Wybran, J., and Fudenberg, H. H., 1973, Thymus-derived rosette forming cells in vamus human disease states: cancer, lymphoma, bacterial and viral infections, and other diseases, J. Clin. Invest. 52: 1026–1032.PubMedCrossRefGoogle Scholar
  245. Wybran, J., Carr, M. C., and Fudenberg, H. H., 1972, The human rosette-forming cell as a marker of a population of thymus-derived cells, J. Clin. Invest. 51: 2537–2543.PubMedCrossRefGoogle Scholar
  246. Wybran, J., Carr, M. C., and Fudenberg, H. H., 1974, Effect of serum on human rosette forming cells in fetuses and adult blood, Clin. Immunol. Immunopathol. 1: 408–412.CrossRefGoogle Scholar
  247. Yefenof, E., Bakaes, T., Einham, L., Emberg, L., and Klein, G., 1978, Epstein-Barr virus (EBV) receptors, complement receptors, and EBV infectibility of different lymphocyte fractions of human peripheral blood. I. Complement receptor distribution and complement binding by separated lymphocyte subpopulations, Cell. Immunol. 35: 34–42.PubMedCrossRefGoogle Scholar
  248. Yodoi, J., Miyama, M., and Masuda, T., 1978, Immunological properties of Fc receptor on lymphocytes. 2. Differentiation from FcR- to FcR+ cells and their functional differences in in vitro antibody response, Cell Immunol. 35: 266–278.PubMedCrossRefGoogle Scholar
  249. Yoshida, T. O., and Andersson, B., 1972, Evidence for a receptor recognizing antigen complexed immunoglobulin on the surface of activated mouse thymus lymphocytes, Scand. J. Immunol. 1: 401–409.PubMedCrossRefGoogle Scholar
  250. Yu, D. T. Y. (1975) Human lymphocyte subpopulations: Early and late rosettes, J. Immunol. 115: 91–93.PubMedGoogle Scholar
  251. Yu, D. T. Y., and Gale, R. P., 1977, Human lymphocyte subpopulations: Rabbit red blood cell rosettes, J. Immunol. Methods 16: 283–289.PubMedCrossRefGoogle Scholar
  252. Zighelboim, J., Gale, R. P., Chin, A., Bonavida, B., Ossorio, R. C., and Farey, J. L., 1974, Antibody dependent cellular cytotoxicity: Cytotoxicity mediated by non-T lymphocytes, Clin. Immunol. Immunopathol. 3: 193–198.PubMedCrossRefGoogle Scholar
  253. Zola, H., 1977, Fractionation of human lymphocytes using rosette formation with papain treated mouse erythrocytes, J. Immunol. Methods 18: 387–390.PubMedCrossRefGoogle Scholar
  254. Zoschke, D. C., and Bach, F. H., 1970, Specificity of antigen recognition by human lymphocytes in vitro, Science 170: 1404–1406.Google Scholar
  255. Zoschke, D. C., and Bach, F. H., 1971a, In vitro elimination of specific immunoreactive cells with 5-bromodeoxyuridine, J. Immunol. Methods 1: 55–63.PubMedCrossRefGoogle Scholar
  256. Zoschke, D. C., and Bach, F. H., 197lb, Specificity of allogeneic cell recognition by human lymphocytes in vitro, Science 171:1350–1352.Google Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Chris D. Platsoucas
    • 1
  • Nicholas Catsimpoolas
    • 1
  1. 1.Biophysics Laboratory, Department of Nutrition and Food ScienceMassachusetts Institute of TechnologyCambridgeUSA

Personalised recommendations