Principles of Continuous Flow Cell Separation in a Circumferential Flow Disposable Channel
Continuous flow centrifugation (CFC) is used to centrifuge large volumes of blood and concentrate cellular or acellular subfractions for extraction. The efficient separation of most blood components requires a system that permits removal of selected elements while returning most of the remaining fractions to the donor/patient. When used in the biological sciences, these systems must be closed and sterile. In 1965, Freireich et al. reported the first use of a closed, sterile, continuous flow centrifuge in man to perform leukocyte extraction. Application growth is based on the fact that these systems allow access to a donor/patient’s entire blood volume for processing and manipulation. A nonexhaustive list of CFC applications is contained in Table 1.
KeywordsAxial Flow Buffy Coat Centrifuge Speed Separation Channel Collection Chamber
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