Latex Particle Size Analysis by Chromatographic Methods: Porous Packed Systems and Detection of Polystyrene

  • D. J. Nagy
  • C. A. Silebi
  • A. J. McHugh


Experimental results are presented for the size separation of polystyrene latexes using a porous packed system of a single large pore size. Parameters such as separation factor, ionic strength, flow rate, axial dispersion, and resolution are considered, and compared to results obtained using nonporous hydrodynamic chromatography (HDC). The porous system shows improved peak separation, however, overall resolution is decreased due to increased axial dispersion. A parallel flow-through bank model is presented to account for the ionic strength behavior of the separation factor in a porous system. Resolution in terms of optimum signal detection is discussed to account for absorption and scattering effects of polystyrene latexes. Overall improvement in HDC signal resolution resulting from optical density measurements in the absorbing region is shown to occur.


Ionic Strength Separation Factor Elution Volume Axial Dispersion Polystyrene Latex 
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Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • D. J. Nagy
    • 1
  • C. A. Silebi
    • 1
  • A. J. McHugh
    • 1
  1. 1.Emulsion Polymers InstituteLehigh UniversityBethlehemUSA

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