Monoamine Oxidase and the Enzymes of Ethanol Metabolism in Rats After Administration of Tetrahydroisoquinolines

  • Yu. M. Ostrovsky
  • M. N. Sadovnik
  • V. I. Satanovskaya
  • M. S. Omelyanchik
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 126)


A mixture of tetrahydroisoquinolines, synthesized by the method of Osswald et al. (1975), was injected intraperitoneally at a dose of 2 mg/kg into rats. After 3 hours, the activity of ethanol-metabolizing enzymes (alcohol dehydrogenase and the microsomal ethanol oxidizing system) and acetaldehyde-metabolizing enzymes (NAD- and NADP-dependent aldehyde dehydrogenases) in liver subcellular fractions, as well as the activity of monoamine oxidase with noradrenaline, tyramine, benzylamine and serotonin as substrates, were investigated in various brain areas. There was observed a decrease in the activity of the microsomal ethanol-oxidizing system and the NAD-dependent mitochondrial aldehyde dehydrogenase with low Km for acetaldehyde, the activity of the other enzymes investigated being invariable. The inhibition of the activity of monoamine oxidase with noradrenaline as a substrate was revealed in the caudate nucleus, cerebellum, truncus cerebri, and cerebral cortex, while only in the cerebral cortex was there revealed an inhibition of monoamine oxidase with the other substrates. The data obtained indicate that tetrahydroisoquinoline alkaloids not only disturb biogenic amine metabolism, but also affect ethanol and acetaldehyde metabolism in animals.


Alcohol Dehydrogenase Monoamine Oxidase Amine Oxidase Ethanol Metabolism Infrared Spectrophotometry 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Balakleevsky, A., Colorimetric method for determination of monoamine oxidase activity. Laborat. dyelo, 3: 151–153, 1976.Google Scholar
  2. Bobbit, J.M., Kiely, J.McN., Khanna, K.L., and Ebermann, R. Synthesis of isoquinolines. III. A new synthesis of 1,2,3,4-tetrahydroisoquinolines. J. Org. Chem., 30: 2247–2250, 1965.CrossRefGoogle Scholar
  3. Bonnichsen, P.K., and Brink, N.G. Liver Alcohol Dehydrogenase in Methods in Enzymology (Eds.) Colowick, S.P., Kaplan, N.O., New York, Acad. Press, Vol. 1: 495–500, 1955.Google Scholar
  4. Cohen, G., and Collins, M.A. Alkaloids from catecholamines in adrenal tissue: possible role in alcoholism. Science, 167: 1749–1751, 1970.PubMedCrossRefGoogle Scholar
  5. Collins, M. Tetrahydroisoquinoline alkaloids from condensation of alcohol metabolites with norepinephrine: preparative synthesis and potential analysis in nervous tissue by gas chromatography. Ann. N.Y. Acad. Sci. 215: 92–97, 1973.PubMedCrossRefGoogle Scholar
  6. Collins, M.A., and Bigdeli, M.G. Tetrahydroisoquinolines in vivo. 1. Rat brain formation of salsolinol, a condensation product of dopamine and acetaldehyde, under certain conditions during ethanol intoxication. Life Sci., 16: 585–602, 1975.PubMedGoogle Scholar
  7. Collins, A.S., Cashaw, J.L., and Davis, V.E. Dopamine-derived tetrahydroisoquinoline alkaloids — inhibitors of neuroamine metabolism. Biochem. Pharmacol., 22: 2327–2348, 1973.Google Scholar
  8. Davis, V.E., and Walsh, M.J. Alcohol, amines and alkaloids: a Possible biochemical basis for alcohol addiction. Science, 167: 1005–1007, 1970.PubMedCrossRefGoogle Scholar
  9. Koivula, T., and Lindros, L.O. Effects of long-term ethanol treatment on aldehyde and alcohol dehydrogenase activities in rat liver. Biochem. Pharmacol., 1917–1942, 1975.Google Scholar
  10. Lahti, R.A. Alcohol, aldehydes and biogenic amines. In ‘Biochemical Pharmacology of Ethanol’ (Eds.) E. Majchrowicz, 1975, Plenum Press, N.Y.-London, 239–253, 1975.CrossRefGoogle Scholar
  11. Lahti, R.A., and Majchrowicz, E. Acetaldehyde: an inhibitor of the enzymatic oxidation of 5-hydroxyindoleacetaldehyde. Biochem. Pharmacol., 18: 535–538, 1969.PubMedCrossRefGoogle Scholar
  12. Lieber, C.E., and Decarli, L.M. Hepatic microsomal ethanol — oxidizing system. J. Biol. Chem., 245: 2505–2512, 1970.PubMedGoogle Scholar
  13. Litterst, C.L., Mimnaugh, E.G., Reagan, R.L., and Gram, T.E. Drug metabolism by microsomes from extrahepatic organs of rat and rabbit prepared by calcium aggregation. Life. Sci., 17: 813–818, 1975.PubMedCrossRefGoogle Scholar
  14. Meyerson, L.R., and Davis, V.E. Purification and characterization of a benzyl-tetrahydroisoquinoline methyltransferase from rat liver. Fed. Proc., 34: 508, 1975.Google Scholar
  15. Osswald, W., Polonia, J., and Poloniaj. M. A. Preparation and pharmacological activity of the condensation product of adrenaline with acetaldehyde. Naunyn-Schmiedeberg’s Arch. Pharmacol., 289: 275–290, 1975.CrossRefGoogle Scholar
  16. Rahwan, R.G., Review Article. Toxic effects of ethanol: possible role of acetaldehyde, tetrahydroisoquinolines, and tetrahydro-beta-carbolines, 34: 3–27, 1975.Google Scholar
  17. Siew, C., Deitrich, R.A., and Erwin, V.G. Localization and characteristics of rat liver mitochondrial aldehyde dehydrogenase. Arch. Biochem. Biophys., 176: 638–649, 1976.PubMedCrossRefGoogle Scholar
  18. Tottmar, S.O.S., Pettersson, H., and Kiessling, K.H. The subcellular distribution and properties of aldehyde dehydrogenases in rat liver. Biochem. J., 135: 577–586, 1973.PubMedGoogle Scholar
  19. Tottmar, O., and Marchner, H. Disulfiram as a tool in the studies on the metabolism of acetaldehyde in rats. Acta Pharmacol, et Toxicol., 38: 366–375, 1976.CrossRefGoogle Scholar
  20. Truitt, E.B.J., and Walsh, M.J. The role of acetaldehyde in the action of ethanol. In ‘Biology of Alcoholism’ (Eds.) B. Kissin and H. Begleiter, Plenum Press, N.Y., 161–195, 1971.CrossRefGoogle Scholar
  21. Walsh, M.J., Davis, V.E., and Yamanaka, T. Tetrahydropapaveroline: an alkaloid metabolite of dopamine in vitro. J. Pharmacol. Exp. Ther., 174: 388–400, 1970.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Yu. M. Ostrovsky
    • 1
  • M. N. Sadovnik
    • 1
  • V. I. Satanovskaya
    • 1
  • M. S. Omelyanchik
    • 1
  1. 1.Dept. Metabolism RegulationByelorussian SSR Academy of SciencesGrodnoUSSR

Personalised recommendations