Half-Lives and Actions of Intracerebrally Injected Isoquinolines

  • Christine L. Melchior
  • Richard A. Deitrich
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 126)


The chronic infusion of the tetrahydroisoquinclines (TIQs), tetrahydropapaveroline (THP) or salsolinol into the cerebral ventricle of the rat induces an increase in ethanol drinking (Myers and Melchior, 1977a,b). Although a dose-response relationship was not shown, a range of doses from 2.0 μg every 15 minutes to 0.4 ng every 30 minutes has proven efficacious (Melchior and Myers, 1977a,b).


Adenylate Cyclase INTRACEREBRALLY Inject Posterior Section Intraventricular Injection Sodium Metabisulfite 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Cohen, G.: Alkaloid products in the metabolism of alcohol and biogenic amines. Biochem Pharmacol. 25:1123–1128, 1976.PubMedCrossRefGoogle Scholar
  2. 2.
    Collins, A.C., Cashaw, J.L. and Davis, V.E.: Dopamine-derived tetrahydroisoquinoline alkaloids-inhibitors of neuroamine metabolism. Biochem. Pharmacol. 22:2237–2348, 1973.CrossRefGoogle Scholar
  3. 3.
    Crout, J.: Inhibition of catechol-0-methyl transferase by pyrogallol in the rat. Biochem. Pharmacol. 6:47–54, 1961.PubMedCrossRefGoogle Scholar
  4. 4.
    Fields, J.Z., Reisine, R.D. and Yamamura, H.I.: Biochemical demonstration of dopaminergic receptors in rat and human brain using [3H] spiroperidol. Brain Res. 136–578-584, 1977.Google Scholar
  5. 5.
    Glowinski, J. and Iverson, L.L.: Regional studies of catecholamines in the rat brain -I. The disposition of [3H] norepinephrine, [3H] dopamine and [3H] dopa in various regions of the brain. J. Neurochem. 13:655–669, 1966.PubMedCrossRefGoogle Scholar
  6. 6.
    Langan, T.A.: Histone phosphorylation: stimulation of adenosine 3′,5′-monophosphate. Science 162:579–580, 1968.PubMedCrossRefGoogle Scholar
  7. 7.
    Leysen, J.E., Gommeren, W. and Laduron, P.M.: Spiperone: A ligand of choice for neuroleptic receptors. I. Kinetics and characteristics of in vitro binding. Biochem. Pharmacol. 27: 307–316, 1978.PubMedCrossRefGoogle Scholar
  8. 8.
    Melchior, C.L. and Myers, R.D.: Preference for alcohol evoked by tetrahydropapaveroline (THP) chronically infused in the cerebral ventricle of the rat. Pharmacol. Biochem. Behav. 7: 19–35, 1977.PubMedCrossRefGoogle Scholar
  9. 9.
    Myers, R.D.: Method for chemical stimulation of the brain: in: Methods in Psychobiology, Vol. 1, R.D. Myers, ed. London: Academic Press 1971a, pp 247–280.Google Scholar
  10. 10.
    Myers, R.D.: General laboratory procedures, in: Methods in Psychobiology Vol. 1, R.D. Myers, ed. London: Academic Press, 1971b, pp 27–65.Google Scholar
  11. 11.
    Myers, R.D. and Melchior, C.L.: Alcohol drinking: abnormal intake caused by tetrahydropapaveroline in brain. Science 196:554–556, 1977a.PubMedCrossRefGoogle Scholar
  12. 12.
    Myers, R.D. and Melchior, C.L.: Differential actions on voluntary alcohol intake of tetrahydroisoquinolines or a β-carboline infused chronically in the ventricle of the rat. Pharmacol. Biochem. Behav. 7:381–392, 1977b.PubMedCrossRefGoogle Scholar
  13. 13.
    Myers, R.D. and Oblinger, M.M.: Alcohol drinking in the rat induced by acute intracerebral infusion of two tetrahydroisoquinolines and a β-carboline. Drug and Alcohol Dependence 2: 469–483, 1977.PubMedCrossRefGoogle Scholar
  14. 14.
    Miller, R., Horn, A., Iversen, L. and Pinder, R.: Effects of dopamine-like drugs on rat striatal adenyl cyclase have implications for CNS dopamine receptor topography.Nature 250:238–241, 1974.PubMedCrossRefGoogle Scholar
  15. 15.
    O’Neill, P.J. and Rahwan, R.G.: Absence of formation of brain salsolinol in ethanol-dependent mice. J. Pharmacol. Exp. Ther. 200:306–313, 1977.PubMedGoogle Scholar
  16. 16.
    Riggin, R., Horn, A., Iversen, L. and Kissinger, P.T.: Determination of tetrahydroisoquinoline alkaloids in biological materials with high performance liquid chromatography. Anal. Chem. 49:530–534, 1977.PubMedCrossRefGoogle Scholar
  17. 17.
    Salomon, Y., Londos, C. and Rodbell, M.: A highly sensitive adenylate cyclase assay. Anal. Biochem. 58:541–548, 1974.PubMedCrossRefGoogle Scholar
  18. 18.
    Sheppard, H. and Burghardt, C.R.: Effect of tetrahydroisoquinoline derivatives on the adenylate cyclases of the caudate nucleus (dopamine-type) and erythrocyte (β-type) of the rat. Res. Commun. Chem. Pathol. Pharm. 8:527–534, 1974.Google Scholar
  19. 19.
    Sheppard, H., Burghardt, C.R. and Teitel, S.: The dopamine-sensitive adenylate cyclase of the rat caudate nucleus. II. a coiriparison with the isoproterenol-sensitive (Beta) adenylate cyclase of the rat erythrocyte for inhibition or stimulation by tetrahydroisoquinolines. Mol. Pharmacol. 12:854–861, 1976.PubMedGoogle Scholar
  20. 20.
    Sporn, J.R. and Molinoff, P.B.: β-adrenergic receptors in rat brain. J. Cyclic Nuc. Res. 2:149–161, 1976.Google Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Christine L. Melchior
    • 1
  • Richard A. Deitrich
    • 1
  1. 1.Department of PharmacologyUniversity of Colorado Medical CenterDenverUSA

Personalised recommendations