Quantification of Polycyclic Aromatic Hydrocarbons in Marine Environmental Samples

  • W. J. Cretney
  • P. A. Christensen
  • B. W. McIntyre
  • B. R. Fowler
Part of the Environmental Science Research book series (ESRH, volume 16)


The potential for use of perdeuterated polycyclic aromatic hydrocarbons (PAHs) in the analysis of marine sediments and organisms by a method that employs a gas chromatography/mass spectrometer (GC/MS) system operating in the multiple ion monitoring (MIM) mode is examined. Questions concerning chemisorption of PAHs on adsorbents, hydrogen-deuterium exchange, interfering compounds, and the separation of deuterated and protiated PAHs by gas or liquid chromatography are considered and experiments that were performed to obtain answers are described. The evidence indicates that deuterium labelled PAHs are useful carriers and internal standards for their unlabelled forms in the analysis of marine organism and sediment samples.


Nominal Mass Isotope Dilution Analysis Polycyclic Aromatic Hydro Isomeric PARs Deuterated Standard 
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  1. Bertilsson, L., A.J. Atkinson, Jr., J. R. Althaus, A. Harfast, J.E. Lindgren, and B. Holmstedt. 1972. Quantitative Determination of 5-Hydroxyindole-3-Acetic Acid in Cerebro-spinal Fluid by Gas Chromatography-Mass Spectrometry. Anal. Chem. 44: 1434–1438.CrossRefGoogle Scholar
  2. Blumer, M., and W.W. Youngblood. 1975. Polycyclic Aromatic Hydrocarbon in Soils and Recent Sediments. Science 188: 53–55.ADSCrossRefGoogle Scholar
  3. Chow, T.J. 1968. Isotope Analysis of Seawater by Mass Spectrometry, Part 1, J. Water Poll. Control Fed. 40: 390–411.Google Scholar
  4. Dunn, B.P. 1976. Techniques for Determination of Benzo(a)pyrene in Marine Organisms and Sediments. Environ. Sci. Techno1. 10: 1018–1021.CrossRefGoogle Scholar
  5. Farrington, J.W., N.M. Frew, P.M. Gschwend, and B.W. Tripp. 1977. Hydrocarbons in Cores of Northwestern Atlantic Coastal and Continental Margin Sediments. Estuar. Coast. Mar. Sci. 5: 793–808.CrossRefGoogle Scholar
  6. Giger, W., and M. Blumer. 1974. Polycyclic Aromatic Hydrocarbons in the Environment: Isolation and Characterization by Chromatography, Visible, Ultraviolet, and Mass Spectrometry. Anal. Chem. 46: 1663–1671.CrossRefGoogle Scholar
  7. Giger, W., and C. Schaffner. 1978. Determination of Polycyclic Aromatic Hydrocarbons in the Environment by Glass Capillary Gas Chromatography. Anal. Chem. 50: 243–249.CrossRefGoogle Scholar
  8. Heck, H.D., R.L. Simon, and M. Anbar. 1977. Isotopic Fractionation in Thin-Layer Chromatography. J. Chromatog. 133: 281–290.CrossRefGoogle Scholar
  9. Hites, R.A., R.L. Laflamme, J.W. Farrington. 1977. Sedimentary Polycyclic Aromatic Hydrocarbons: The Historical Record. Science. 198: 829–831.ADSCrossRefGoogle Scholar
  10. Holland, J.F., C.C. Sweeley, R.E. Thrush, R.E. Teets, and M.A. Bieber. 1973. On-Line Computer Controlled Multiple Ion Detection in Combined Gas Chromatography-Mass Spectrometry. Anal. Chem. 45: 308–314.CrossRefGoogle Scholar
  11. Howard, J.W., T. Fazio, R.W. White, and B.A. Klimeck. 1968. Extraction and Estimation of Polycyclic Aromatic Hydrocarbons in Total Diet Composites. J. Ass. Offic. Anal. Chem., 51: 122–129.Google Scholar
  12. Howard, J.W., R.T. Teague, Jr., R.W. White, and B.E. Fry, Jr. 1966. Extraction and Estimation of Polycyclic Aromatic Hydrocarbons in Smoked Foods. I. General Method. J. Ass. Offi. Anal. Chem. 49: 595–617.Google Scholar
  13. Jancso, G., and W.A. Van Hook. 1974. Condensed Phase Isotope Effects (Especially Vapor Pressure Isotope Effects). Chem. Rev. 74: 689–750.CrossRefGoogle Scholar
  14. Janini, G.M., G.M. Muschik, J.A. Schroer, and W.L. Zielinski, Jr. 1976. Gas-Liquid Chromatographic Evaluation and Gas Chromato-graphy/Mass Spectrometric Application of New High Temperature Liquid Crystal Stationary Phases for Polycyclic Aromatic Hydrocarbon Separations. Anal. Chem. 48: 1879–1883.CrossRefGoogle Scholar
  15. Laflamme, R.E., and R.A. Hites. 1978. The Global Distribution of Polycyclic Aromatic Hydrocarbons in Recent Sediments. Geochim. Cosmochim. Acta. 42: 289–303.ADSCrossRefGoogle Scholar
  16. Lindgren, B.W., and G.W. Elrath. 1969. “Introduction to Probability and Statistics”, p. 179. Collier-Macmillan Ltd., London.Google Scholar
  17. Laidler, K.J. 1965. “Chemical Kinetics”, pp.97–98. McGraw-Hill Book Co., Toronto.Google Scholar
  18. Lee, M.L., and R.A. Hites. 1976. Mixed Charge Exchange-Chemical Ionization and Mass Spectrometry of Polycyclic Aromatic Hydrocarbons. J. Amer. Chem. Soc. 99: 2008.CrossRefGoogle Scholar
  19. Lee, R.F. 1977. Accumulation and Turnover of Petroleum Hydrocarbons in Marine Organisms, Chapter 6. In: D.A. Wolfe (ed.) “Fate and Effects of Petroleum Hydrocarbons in Marine Ecosystems and Organisms”. Pergamon Press, New York.Google Scholar
  20. Middleditch, B.S., and B. Basile. 1976. Deuterated Analogs as Internal Standards for the Quantitation of Environmental Alkanes by Gas Chromatography. Anal Lett. 9: 1031–1034.CrossRefGoogle Scholar
  21. Pancirov, R.J., and R.A. Brown. 1977. Polynuclear Aromatic Hydrocarbons in Marine Tissues. Environ. Sci. Technol., 11: 989–992.CrossRefGoogle Scholar
  22. Patterson, C.C., and D.M. Settle. 1976. The Reduction of Orders of Magnitude Errors in Lead Analyses of Biological Materials and Natural Waters by Evaluating and Controlling the Extent and Sources of Industrial Lead Contamination Introduced During Sample Collecting, Handling, and Analysis, pp. 321–351. In: “Proceedings of the 7th I.M.R. Symposium.” NBS Spec. Publ. 422. U.S. Gov. Printing Office, Washington, D.C., U.S.A. 20402.Google Scholar
  23. Payne, J.F. 1977. Mixed Function Oxidases in Marine Organisms in Relation to Petroleum Hydrocarbon Metabolism and Detection. Mar. Poll. Bull. 8: 112–116.CrossRefGoogle Scholar
  24. Risebrough, R.W., B.W. De Lappe, and T.T. Schmidt. 1976. Bioaccumulation Factors of Chlorinated Hydrocarbons Between Mussels and Seawater. Mar. Poll. Bull. 7: 225–228.CrossRefGoogle Scholar
  25. Snowdon, L.R., and E. Peake. 1978. Gas Chromatography of High Molecular Weight Hydrocarbons with an Inorganic Salt Eutectic Column. Anal. Chem. 50: 379–381.CrossRefGoogle Scholar
  26. Snyder, L.R. 1963. Linear Elution Adsorption Chromatography. VI. Deactivated Florisil as Adsorbent. J. Chromatog. 12: 488–509.Google Scholar
  27. Stich, H.F., A.B. Acton, and C.R. Forrester. 1976. Fish Tumors and Sublethal Effects of Pollutants. J. Fish Res. Board Can. 33: 1993–2001.Google Scholar
  28. Tanaka, N., and E.R. Thornton. 1976. Isotope Effects in Hydrophobic Binding Measured by High-Pressure Liquid Chromatography. J. Amer. Chem. Soc. 98: 1617–1619.CrossRefGoogle Scholar
  29. Vandermeulen, J.H., and W.R. Penrose. 1978. Absence of Aryl Hydrocarbon Hydroxylase (AHH) in Three Marine Bivalves. J. Fish. Res. Board Can. 35: 643–647.CrossRefGoogle Scholar
  30. Van Hook, W.A. 1969. Isotope Separation by Gas Chromatography, pp. 99–118. In: “Isotope Effects in Chemical Processes”. Adv. Chem. Ser. No. 89, American Chemical Society, Washington, D.C.CrossRefGoogle Scholar
  31. Wasik, S.P., and W. Tsang. 1970. Determination of Trace Amounts of Contaminants in Water by Isotope Dilution Gas Chromatography. Anal. Chem. 42: 1649–1651.CrossRefGoogle Scholar
  32. Wong, C.S., W.J. Cretney, R.W. Macdonald, and P. Christensen. 1976. Hydrocarbon Levels in the Marine Environment of the Southern Beaufort Sea. In: “Beaufort Sea Technical Report No.38”. Beaufort Sea Project, Institute of Ocean Sciences, Patricia Bay, P.O. Box 6000, Sidney, B.C., Canada V8L 4B2. Unpublished manuscript.Google Scholar
  33. Youngblood, W.W., and M. Blumer. 1975. Polycyclic Aromatic Hydrocarbons in the Environment: Homologous Series in Soils and Recent Marine Sediments. Geochim. Cosmochim. Acta. 39: 1303–1314.ADSCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • W. J. Cretney
    • 1
  • P. A. Christensen
    • 1
  • B. W. McIntyre
    • 1
  • B. R. Fowler
    • 1
  1. 1.Institute of Ocean SciencesSidneyCanada

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