Oncogenic Transformation of Mammalian Cells by Chemicals and Viral-Chemical Interactions

  • Bruce C. Casto
Part of the Environmental Science Research book series (ESRH, volume 15)


Short-term tests for the identification of potential chemical carcinogens are urgently needed. Presently, the systems receiving the most attention for prediction of carcinogenic activity are the mutagenesis assays in microbial cells. Eventually, a battery of in vitro tests should be available that are reliable, rapid, inexpensive and generate a low percentage of false positives and no false negatives. In vitro mammalian cell systems, using fibroblast-like cells from hamster, rat, mouse, guinea pig, and human subjects have been used for assays of chemical carcinogens (Table 1). These assays show that the above cell types can be reproducibly transformed by various carcinogens and, especially with hamster cells, the capacity to transform shown to correlate with the in vivo activity of known negative or positive chemicals.


Natl Cancer Inst Soft Agar Embryo Cell Mass Culture Chemical Carcinogen 
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Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • Bruce C. Casto
    • 1
  1. 1.BioLabs, Inc.NorthbrookUSA

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