Mutagenic Analysis of Complex Samples of Aqueous Effluents, Air Particulates, and Foods
Opportunities and problems arise when the Ames mutagenesis technique is applied to the analysis of samples, such as those derived from the environment, which are mixtures of unknown compounds that may or may not include mutagens. The chief advantage of this application of the method is well known: one can use it as a rapid, inexpensive, biological screen capable of detecting mutagens by their biological effect. This makes it possible to avoid the very difficult task of detecting and identifying all of the numerous organic compounds that may occur in such a sample in order to compare them with a list of known mutagens. The chief disadvantage of this approach is that one is “flying blind,” so to speak, unaware in advance of what types of compounds are present, their concentrations, and their possible interference with the test.
KeywordsMutagenic Activity Beef Extract Ames Test Ground Beef Methylene Chloride Extract
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- 2.Ames BN, McCann J, Yamasaki E: Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test. Mutat Res 31:347–364, 1975Google Scholar
- 3.Commoner B: Reliability of bacterial mutagenesis techniques to distinguish carcinogenic and noncarcinogenic chemicals. Washington DC, US Environmental Protection Agency Publ. No. EPA-600/1–76–022, p 104, 1976Google Scholar
- 6.Nebert DW, Feton JS: Evidence for the activation of 3-methylcholanthrene as a carcinogen in. vivo and as a mutagen in vitro by P1-450 from inbred strains of mice. In: Cytochromes P-450 and b5, Structure, Function and Interaction (Cooper DW, Rosenthall O, Snyder R, Witmer C, eds.). New York, Plenum, pp 127–149, 1975Google Scholar
- 7.Sugimura T, Nagao M, Kawachi T, Honda M, Yahagi T, Seino Y, Sato S, Matsukura N, Matsushima T, Shirai A, Sawamura M, Matsumoto H: Mutagen-carcinogens in food with special reference to highly mutagenic pyrolitic products in broiled foods. In: Origins of Human Cancer (Hiatt HH, Watson JD, Winsten JA, eds.). Book B, Cold Spring Harbor, NY, Cold Spring Harbor Laboratory, pp 1561–1577, 1977Google Scholar