In Vitro Activation of Cigarette Smoke Condensate Materials to Their Mutagenic Forms

  • R. E. Kouri
  • K. R. Brandt
  • R. G. Sosnowski
  • L. M. Schechtman
  • W. F. Benedict
Part of the Environmental Science Research book series (ESRH, volume 15)


Cigarette smoke is a complex mixture composed of 5,000–10,000 different chemicals in the particulate phase, of which about 3,000 have been identified (1), and 1,000–2,000 chemicals in the gas phase. The particulate fraction contains many chemicals that are capable of inducing cancer in model test systems. Among these chemicals are certain polycyclic aromatic hydrocarbons (PAH) [e.g., benzo(a)pyrene (BP), dibenz(a,h)anthracene, and benz(a)anthracene]; certain nitrosamines (e.g., diethylnitrosamine and nitrosopiperidine); and certain aromatic amines [e.g., 2-napthylamine (2-NA) and 2-aminofluorenes (2-AF)] (see review 2). These chemicals are normally at levels approaching 0.5–20 ng/cigarette. The particulate phase also contains chemicals that are capable of promoting carcinogenesis (2–4). The level of these chemicals (e.g., catachol) are on the order of 10,000–100,000 ng/cigarette. Thus, there is a problem in determining not only whether cigarette smoke plays an active role in smoke-associated cancers in man, but also if this association occurs at the level of initiation and/or promotion of cancer.


Aromatic Amine Cigarette Smoke Condensate Aryl Hydrocarbon Hydroxylase Revertant Coloni Aryl Hydrocarbon Hydroxylase Activity 
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Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • R. E. Kouri
    • 1
  • K. R. Brandt
    • 1
  • R. G. Sosnowski
    • 1
  • L. M. Schechtman
    • 1
  • W. F. Benedict
    • 2
  1. 1.Microbiological AssociatesDepartment of Biochemical OncologyBethesdaUSA
  2. 2.Children’s Hospital of Los AngelesLos AngelesUSA

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