The Cholinergic System in the Chicken Retina: Cellular Localization and Development
The cellular localization of the cholinergic system of the chicken retina was determined by means of freeze-drying and dry autoradiography following incubation with [3H]choline under conditions favoring high-affinity uptake. The cholinergic cells were localized to the inner nuclear and ganglion cell layers, and they extended processes in two bands in the inner plexiform layer. During embryogenesis in the chick, the cholinergic system was found to develop in two stages; the first occurs relatively early in retinal differentiation and is associated with increased ACh synthesis and storage and with a large rise in CAT activity; and the second occurs just before hatching, coincident with synaptogenesis and the appearance of visual function, and is associated with further increases in ACh synthesis and storage and with the development of high-affinity choline uptake.
KeywordsCholinergic System Ganglion Cell Layer Plexiform Layer Choline Acetyltransferase Outer Plexiform Layer
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