The Effect of Cyclophosphamide on a Secondary Ocular Immune Response in Rabbits
Our previous experiments (7) showed that cyclophosphamide (Cytoxan) suppressed the immune response of rabbits to intravitreally injected bovine gamma globulin (BGG). Suppression of the 14 day plaque forming cell (PFC) response occurred when treatment was begun as late as 5 days after immunization. The 21 day PFC response and antibody titers were suppressed in rabbits treated from day 0 or day 2 through day 13. When treatment began later, the 21 day response was significantly depressed, but some recovery occurred. Smaller Cytoxan doses (50 mg per injection) were not as immunosuppressive as 100 mg doses. Shorter treatment (days 2 through 8) was less effective than treatment through day 13.
KeywordsAntibody Titer Aqueous Humor Intravitreal Injection Vitreous Humor Secondary Response
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