Modulation of T Cells and Macrophages by Cholera Toxin Treatment in Vivo and in Vitro
Cholera toxin (CT), the enterotoxin derived from pathogenic strains of Vibrio cholerae, is a potent modulator of immune responses, both in vivo and in vitro (1, 2, 5, 6, 7). Mice injected with CT simultaneously with an agent such as sheep erythrocytes (SRBC) show markedly enhanced antibody responses, whereas mice pretreated with CT 2 or 3 days before challenge immunization show marked immunosuppression. These effects have been attributed to the influence of CT on the adenyl cyclase system. In vitro studies in this laboratory showed that CT induces only enhanced antibody responses when added to cultures of normal splenocytes, either simultaneously with antigen or 2 days earlier (3). Thus in vivo immunosuppression may be due to an indirect mechanism. In the present studies, spleen cells from CT pretreated mice were tested for effects on various cell classes and their responsiveness in vitro as compared to in vivo.
KeywordsSpleen Cell Cholera Toxin Challenge Immunization Cell Class Plaque Form Cell
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