Effect of Proteinases on EA (IgG)-Binding Receptors of Rat Macrophages
Reports about the proteinase sensitivity of macrophage receptors for IgG (Fc-receptor) are equivocal. Sorkin (10) studying binding of cytophilic antibody to rabbit splenic macrophages found the binding diminished after treatment with proteolytic enzymes. Other authors, however, found an enhanced (1, 2) or unchanged (4, 7, 9) Fc-receptor activity after proteinase treatment of macrophages from various species. On mouse macrophages 2 different Fc-receptors for rabbit IgG were reported, 1 for aggregated IgG being trypsin resistant, another for monomeric mouse IgG2a being sensitive to digestion by trypsin (3, 11).
KeywordsProteolytic Enzyme Proteinase Treatment Rosette Form Cell Pronase Digestion Proteinase Sensitivity
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