Evidence that Phagocytosing Chicken Polymorphonuclear Leukocytes Generate Hydrogen Peroxide and Superoxide Anion
Polymorphonuclear leukocytes (PMN) are known to produce hydrogen peroxide during the respiratory stimulation associated with the ingestion of particles (9,10,19,24). The oxygen taken up by the cell is univalently reduced to superoxide anion (O2 −) which, in turn, dismutates with formation of hydrogen peroxide. The fate of hydrogen peroxide in these cells is dual, a) it may be destroyed within the cell by enzymes such as myeloperoxidase, catalase or glutathione peroxidase, and/or b) it may leak out of the cell, the amount of peroxide released depending at least on the rate of formation and the efficiency of the intracellular H2O2 destroying systems.
KeywordsOxygen Uptake H202 Production Vanillic Acid NADPH Oxidase Activity Homovanillic Acid
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