Association of Some Metabolic Activities of Leukocytes with the Immune Response

  • R. R. Strauss
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 121B)


Phagocytosis is a physiological function of many different mammalian cell types. These phagocytic cells remove dead cells, tissue, and foreign substances from the body by engulfment and digestion of these materials. Some phagocytic cells, notably the neutrophils, monocytes and macrophages, also serve as primary factors in the host-defense mechanism by engulfing, killing and digesting pathogenic microorganisms. Macrophages have also been implicated in the killing of tumor cells and the humoral immune response. Stimulation of a number of biochemical and metabolic parameters has been associated with phagocytosis. These include increased glucose utilization, 114C-glucose oxidation, 614C-glucose oxidation, oxygen consumption, NADPH oxidase activity, peroxidase activity, superoxide anion production, H2O2 production and chemoluminescence. These biochemical and metabolic activities of phagocytes and their relationship to particle engulfment and intracellular microbicidal activity have been reviewed in detail by Karnovsky (11) and others (23). This report will be concerned with the association of some metabolic activities of mouse macrophages and the immune response. It seems as if some of the metabolic activities that have been related to phagocytosis and killing of microorganisms by macrophage and neutorphils may also be associated with cellular and humoral immunity.


Trypan Blue Spleen Cell NADPH Oxidase Activity Sheep Erythrocyte Spleen Weight 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Atwal, O. S. Experimentia 23 (1967) 185.CrossRefGoogle Scholar
  2. 2.
    Bandmann, U., Rydgren, L. and Norberg, B. Exptl. Cell Res. 88 (1974) 63.PubMedCrossRefGoogle Scholar
  3. 3.
    Beck, F., Lloyd, J. and Griffiths, A. Science (1967) 157.Google Scholar
  4. 4.
    Boxer, L. A., Baehner, R. L. and Davis, J. J. Cell. Physiol. 91 (1977) 89.CrossRefGoogle Scholar
  5. 5.
    Brown, J. Metabolism 11 (1962) 1098.PubMedGoogle Scholar
  6. 6.
    Cohen, H. J. Experientia 29 (1973) 1285.Google Scholar
  7. 7.
    Friedman, H. and Kately, J. R. Proc. Soc. Exp. Biol. Med. 147 (1974) 460.Google Scholar
  8. 8.
    Hirschhorn, Rochelle, Brittinger, et al. In: Proceedings of the 3rd annual leukocyte culture conference (Ed. W. O. Rieke) N. Y., 639.Google Scholar
  9. 9.
    Hirschhorn, R., Brittinger, G., Hirschhorn, K. and Weissman, G. J. Cell. Biol. 37 (1968) 412.CrossRefGoogle Scholar
  10. 10.
    Jerne, N. K. and Nordin, A. A. Science 140 (1963) 405.Google Scholar
  11. 11.
    Karnovsky, M. L. Seminars Hemat. 5 (1968) 156.Google Scholar
  12. 12.
    Karnovsky, M. L., Drath, D. and Lazdins, J. Plenum Press, New York, 121 (1976).Google Scholar
  13. 13.
    Kripke, M. L., Norbury, K. C., Gruyo, E. and Hibbs, J. B., Jr. Infect. Immun. 17 (1977) 121.PubMedGoogle Scholar
  14. 14.
    Marbrook, J. Lancet II (1967) 1279.CrossRefGoogle Scholar
  15. 15.
    Michl, J., Ohlbaum, D. J. and Silverstein, S. C. J. Exptl. Med. 144 (1976) 1461.Google Scholar
  16. 16.
    Oliver, J. M., Albertini, D. F. and Berlin, R. D. J. Cell Biol. 71 (1976) 921.PubMedCrossRefGoogle Scholar
  17. 17.
    Oliver, J. M., Berlin, R, D., Baehner, R. L. and Boxer, L. K. Brit. J. Haematol. 37 (1977) 311.CrossRefGoogle Scholar
  18. 18.
    Patriarca, P., Dri, P., Kakinuma, K. and Rossi, P. Mol. Cell. Biochem. 12 (1976) 137.CrossRefGoogle Scholar
  19. 19.
    Polgar, P. R., Foster, J. M. and Cooperband, S. R. Exptl. Cell Res. 49 (1968) 231.PubMedCrossRefGoogle Scholar
  20. 20.
    Sagone, A. L., Jr., LoBuglio, A. F. and Balcerzak, S. P. Cell. Immunol. 14 (1974) 443.PubMedCrossRefGoogle Scholar
  21. 21.
    Shults, F. S. and Woodward, J. M. Canad. J. Microbiol. 13 (1967) 795.Google Scholar
  22. 22.
    Stossel, T. P. and Pollard, T. D. J. Biol. Chem. 248 (1973) 82–88.Google Scholar
  23. 23.
    Stossel, T. P., N. E. J. Med. 290 (1974) 717.CrossRefGoogle Scholar
  24. 24.
    Strauss, R. R., Paul, B. B., Jacobs, A. A. and Sbarra, A. J. Infect. Immun. 5 (1972) 114.PubMedGoogle Scholar
  25. 25.
    Strauss, R. R., Jacobs, A. A., Paul, B. B. and Sbarra, A. J. RES 11 (1972) 277.Google Scholar
  26. 26.
    Strauss, R. R., Friedman, H., Mills, L. and Zayon, G. Infect. Immun. 15 (1977) 197.PubMedGoogle Scholar
  27. 27.
    Strauss, R. R., Patel, N. and Patel, P. J. Reticuloendothel. Soc. 22 (1977) 533.Google Scholar
  28. 28.
    Zurier, R. B., Weissmann, G., Hoffstein, S., Kammerman, S. and Tai, H. H. J. Clin. Invest. 53 (1974) 297.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • R. R. Strauss
    • 1
  1. 1.Northern DivisionAlbert Einstein Medical CenterPhiladelphiaUSA

Personalised recommendations