Association of Some Metabolic Activities of Leukocytes with the Immune Response
Phagocytosis is a physiological function of many different mammalian cell types. These phagocytic cells remove dead cells, tissue, and foreign substances from the body by engulfment and digestion of these materials. Some phagocytic cells, notably the neutrophils, monocytes and macrophages, also serve as primary factors in the host-defense mechanism by engulfing, killing and digesting pathogenic microorganisms. Macrophages have also been implicated in the killing of tumor cells and the humoral immune response. Stimulation of a number of biochemical and metabolic parameters has been associated with phagocytosis. These include increased glucose utilization, 114C-glucose oxidation, 614C-glucose oxidation, oxygen consumption, NADPH oxidase activity, peroxidase activity, superoxide anion production, H2O2 production and chemoluminescence. These biochemical and metabolic activities of phagocytes and their relationship to particle engulfment and intracellular microbicidal activity have been reviewed in detail by Karnovsky (11) and others (23). This report will be concerned with the association of some metabolic activities of mouse macrophages and the immune response. It seems as if some of the metabolic activities that have been related to phagocytosis and killing of microorganisms by macrophage and neutorphils may also be associated with cellular and humoral immunity.
KeywordsTrypan Blue Spleen Cell NADPH Oxidase Activity Sheep Erythrocyte Spleen Weight
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- 3.Beck, F., Lloyd, J. and Griffiths, A. Science (1967) 157.Google Scholar
- 6.Cohen, H. J. Experientia 29 (1973) 1285.Google Scholar
- 7.Friedman, H. and Kately, J. R. Proc. Soc. Exp. Biol. Med. 147 (1974) 460.Google Scholar
- 8.Hirschhorn, Rochelle, Brittinger, et al. In: Proceedings of the 3rd annual leukocyte culture conference (Ed. W. O. Rieke) N. Y., 639.Google Scholar
- 10.Jerne, N. K. and Nordin, A. A. Science 140 (1963) 405.Google Scholar
- 11.Karnovsky, M. L. Seminars Hemat. 5 (1968) 156.Google Scholar
- 12.Karnovsky, M. L., Drath, D. and Lazdins, J. Plenum Press, New York, 121 (1976).Google Scholar
- 15.Michl, J., Ohlbaum, D. J. and Silverstein, S. C. J. Exptl. Med. 144 (1976) 1461.Google Scholar
- 21.Shults, F. S. and Woodward, J. M. Canad. J. Microbiol. 13 (1967) 795.Google Scholar
- 22.Stossel, T. P. and Pollard, T. D. J. Biol. Chem. 248 (1973) 82–88.Google Scholar
- 25.Strauss, R. R., Jacobs, A. A., Paul, B. B. and Sbarra, A. J. RES 11 (1972) 277.Google Scholar
- 27.Strauss, R. R., Patel, N. and Patel, P. J. Reticuloendothel. Soc. 22 (1977) 533.Google Scholar