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Biophysical Spectroscopy in the Visible and Ultraviolet Using Synchrotron Radiation

  • John Clark Sutherland
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 25)

Abstract

Spectroscopy in the ultraviolet and visible regions of the electromagnetic spectrum is extremely important in the study of biological materials. These lectures consider the applications of synchrotron radiation (SR) to these regions. They are limited to measurements which do not make use of the time structure inherent in SR. Comparisons of SR with conventional sources suggests that the greatest improvements will be realized in the far and vacuum ultraviolet regions -- wavelengths less than about 300 nm. Consideration of the transitions of the valence electrons of most organic and biologically important materials indicate that wavelengths less than about 120 nm will not be especially informative in studies of the structure and function of biological materials (although they are important for understanding the interactions between these materials and high energy electrons). In addition, spectroscopic experiments at wavelengths less than 105 nm become more difficult because of the loss of window materials and surfaces with high normal incidence reflectance. The characteristic visible and ultraviolet absorption bands of proteins, nucleic acids and sugars are reviewed. Important spectroscopic techniques such as absorption, natural and magnetic circular dichroism, fluorescence and various fluorescence polarization spectroscopies are described and their potential use in the far and vacuum ultraviolet (120–300 nm) using synchrotron sources is discussed.

Keywords

Circular Dichroism Synchrotron Radiation Absorption Spectroscopy Aromatic Amino Acid Storage Ring 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • John Clark Sutherland
    • 1
  1. 1.Biology DepartmentBrookhaven National LaboratoryUptonUSA

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