Plasma Protein Survey

  • Samuel Natelson
  • Ethan A. Natelson


When blood is drawn, allowed to clot, and centrifuged, the supernatant obtained is called serum. Serum, in addition to other components, contains all the proteins of the blood other than those involved in the clotting mechanism (mainly fibrinogen) and the cell proteins. If the blood is mixed with an anticoagulant, such as heparin, or a calcium complexing agent, such as sodium fluoride or ethylene diamine tetraacetate, and then centrifuged, the supernatant obtained is called plasma and contains all the proteins of serum, plus fibrinogen and other clotting factors. The proteins of the plasma may be divided into three main categories: albumin, the clotting factors (including fibrinogen), and the globulins. The globulins include the proteins of the complement system, the immunoglobulins, and numerous other proteins with special functions (Table 3.1).


Multiple Myeloma Cellulose Acetate Clotting Factor Transference Number Electrophoretic Pattern 
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  1. 1.
    Hittorf, W., On the migration of ions during electrolysis, Pogg. Ann. 89: 177–211 (1853).Google Scholar
  2. 2.
    Noyes, A. A., and Falk, K. G., The properties of salt solutions in relation to the ionic theory. II. Electrical transference numbers, J. Am. Chem. Soc. 33: 1436–1460 (1911).CrossRefGoogle Scholar
  3. 3.
    von Elissafoff, G., The Influence of electrolytes on electro-endosmosis, Z. Physik. Chem. 79: 385–420 (1912).Google Scholar
  4. 4.
    Wiedermann, G., On the movement of liquids in the closed circuit of the galvanic pile, Pogg. Ann. 87: 321–352 (1856).Google Scholar
  5. 5.
    Longsworth, L. G., Transference numbers of aqueous solutions of potassium chloride, sodium chloride, lithium chloride and hydrochloric acid at 25° by the moving-boundary method, J. Am. Chem. Soc. 54: 2741–2758 (1932).CrossRefGoogle Scholar
  6. 6.
    Tiselius, A., A new apparatus for electrophoretic analysis of colloidal mixtures, Trans. Faraday Soc. 33: 524–531 (1937).CrossRefGoogle Scholar
  7. 6a.
    Tiselius, A., Electrophoresis of serum globulin, electrophoretic analysis of normal and immune serum, Biochem. J. 31: 1464–1477 (1937).Google Scholar
  8. 7.
    Findlay, A., Practical Physical Chemistry, 4th ed., Longman’s Green, New York (1925), p. 186;Google Scholar
  9. Findlay, A., Chem. News 100: 185 (1909).Google Scholar
  10. 8.
    Longsworth, L. G., Recent advances in the study of proteins by electrophoresis, Chem. Rev. 30: 323–340 (1942).CrossRefGoogle Scholar
  11. 9.
    Abramson, H. A., Influence of size, shape, and conductivity on cataphoretic mobility and its biological significance, J. Phys. Chem. 35: 289–308 (1931).CrossRefGoogle Scholar
  12. 10.
    Cremer, H. D., and Tiselius, A., Electrophoresis of albumin on filter paper, Biochem. Z. 320: 273–283 (1950).Google Scholar
  13. 11.
    Adlersburg, D., Bossak, E. T., Sher, I. H., and Sobotka, H., Electrophoresis and monomolecular layer studies with serum lipoproteins, Clin. Chem. 1: 18–33 (1955).Google Scholar
  14. 12.
    Kohn, J., A cellulose acetate supporting medium for zone electrophoresis, Clin. Chim. Acta 2: 297–303 (1957).CrossRefGoogle Scholar
  15. 13.
    Kunkel, H. G., and Wallenius, G., New hemoglobin in normal adult blood, Science 122: 288 (1955).CrossRefGoogle Scholar
  16. 14.
    Smithies, O., Zone electrophoresis in starch gel: group variations in serum proteins of normal human adults, Biochem. J. 61: 629–641 (1955).Google Scholar
  17. 15.
    Ornstein, L., Disc electrophoresis. I. Background and theory, Ann. N.Y. Acad. Sci. 121: 321–349 (1964).CrossRefGoogle Scholar
  18. 16.
    Laurell, C. B., Electrophoresis, specific protein assays, or both in measurement of plasma proteins, Clin. Chem. 19: 99–102 (1973).Google Scholar
  19. 17.
    Grabar, P., and Williams, C. A., Method permitting the combined study of the electrophoretic and immunochemical properties of a mixture of proteins, Biochim. Biophys. Acta 10: 193–194 (1953).CrossRefGoogle Scholar
  20. 18.
    Laurell, C. B., Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies, Anal. Biochem. 15: 45–52 (1966).CrossRefGoogle Scholar
  21. 19.
    Raisys, V., and Aryan, D., Determination of proteins in biological fluids by electroimmunodiffusion and two-dimensional immunoelectrophoresis, Clin. Chem. 17: 745–750 (1971).Google Scholar
  22. 20.
    Koepke, J. A., Thoma, J. E., and Schmidt, R. M., Identification of human hemoglobins by use of isoelectric focusing in gel, Clin. Chem. 21: 1953–1955 (1975).Google Scholar
  23. 21.
    Pesce, A., Hsu, A., Kornhauser, C., Sethi, K., Ooi, R. S., and Pollack, V., Methods for measuring the concentration of urinary proteins according to their molecular size category, Clin. Chem. 22: 667–672 (1976).Google Scholar
  24. 22.
    Svedberg, T., and Nichols, J. B., Application of the oil turbine type of centrifuge to the study of the stability region of carbon monoxide hemoglobin, J. Am. Chem. Soc. 49: 2920–2934 (1927).CrossRefGoogle Scholar
  25. 23.
    McFarlane, A. S., Ultracentrifugal analysis of normal and pathological serum fractions, Biochem. J. 29: 1204–1229 (1935).Google Scholar
  26. 24.
    Pederson, K. O., Ultracentrifugal Studies on Serum and Serum Fractions, Almquist and Wiksell, Stockholm (1945).Google Scholar
  27. 25.
    Gofman, J. W., Lingdren, F. T., Elliott, A., Mantz, W., Hewitt, J. E., Strisower, B., Herring, V., and Lyon, T. P., Role of lipides and lipoproteins in atherosclerosis, Science 111: 166–171 (1950).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Samuel Natelson
    • 1
  • Ethan A. Natelson
    • 2
  1. 1.Department of Environmental Practice, College of Veterinary MedicineUniversity of TennesseeKnoxvilleUSA
  2. 2.University of Texas Medical School and St. Joseph HospitalHoustonUSA

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