Prealbumin I: Iodothyronine-Binding Proteins

  • Samuel Natelson
  • Ethan A. Natelson


Prealbumin is predominantly a complex of thyroxine-binding prealbumin and retinol-binding protein. Thyroxine-binding prealbumin is well characterized and will be discussed first. For convenience, the nature of thyroxine binding globulin will also be discussed since the function of these two proteins is interrelated.


Basal Metabolic Rate Free Thyroxine Human Thyroxin Binding Globulin 
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Selected Reading

  1. McDowell, D. R., An evaluation of serum thyroxine binding globulin as a routine test of thyroid function, Ann. Clin. Biochem. 16: 81–85 (1979).Google Scholar
  2. Jackson, G. L., Burkle, F. W., Flickinger, F. W., and Kwee, H. G., Thyroid function tests—What should be ordered?, Phila. Med. 74: 143–147 (1978).Google Scholar
  3. Werner, S. C., and Ingbar, S. H., Thyroid, 3rd ed., Harper, New York (1978).Google Scholar
  4. Harland, W. A., and Orr, J. S., Thyroid Hormone Metabolism, Academic Press, New York (1975).Google Scholar
  5. Robbins, J., Thyroxine-binding proteins, Prog. Clin. Biol. Res. 5: 331–355 (1976).Google Scholar
  6. Sterling, K., and Lazarus, J. H., The thyroid and its control, Ann. Rev. Physiol. 39: 349–371 (1977).CrossRefGoogle Scholar
  7. Gershengorn, M. C., Lippoldt, R. E., Edelhoch, H., and Robbins, J., Structure and stability of human thyroxine-binding globulin, J. Biol. Chem. 252: 3719–3723 (1977).Google Scholar
  8. Tabachnik, M., and Korcek, L., Thyroxine protein interactions: Binding constants for thyroxine analogs with the thyroxin binding site on human thyroxine binding globulin. Biochem. Biophys. Acta 537: 169–175 (1978).Google Scholar
  9. Wellby, M. L., The laboratory diagnosis of thyroid disorders, Ada. Clin. Chem. 13: 103–172 (1976).CrossRefGoogle Scholar
  10. Lorenz, M. D., and Cornelius, L. M., Laboratory diagnosis of endocrinological disease, Vet. Clin. North Am. 6: 637–722 (1976).Google Scholar
  11. Pain, R. W., In vitro testing of thyroid function: A review, Pathology 7:1–12 (1975).CrossRefGoogle Scholar
  12. Gottschalk, A., and Pochen, E. J., Eds., Diagnostic Nuclear Medicine, Williams and Wilkens, Baltimore (1976), pp. 240–250.Google Scholar


  1. 1.
    Siebert, F. B., and Nelson, J. W., Electrophoretic study of the blood protein response in tuberculosis, J. Biol. Chem. 143: 29–38 (1942).Google Scholar
  2. 2.
    Schultze, H. E., Heimburger, N., and Frank, G., The amino acid composition of human pre-albumin (try) and that of human and bovine serum albumin, Biochem. Z. 336: 388–396 (1962).Google Scholar
  3. 3.
    Gonzalez, G., and Offord, R. E., The subunit structure of prealbumin, Biochem. J. 125: 309–317 (1971).Google Scholar
  4. 4.
    Kanda, Y., Goodman, D. S., Canfield, R. E., and Morgan, F. J., The amino acid sequence of human plasma prealbumin, J. Biol. Chem. 249: 6796–6805 (1974).Google Scholar
  5. 5.
    Branch, W. T., Jr., Robbins, J., and Edelhoch, H., Thyroxin-binding pre-albumin. Conformation in urea and guanidine, Arch. Biochem. Biophys. 152: 144–151 (1971).CrossRefGoogle Scholar
  6. 6.
    Blake, C. C. F., Geisow, M. J., Swan, I. D., Rerat, C., and Rerat, B., Structure of human plasma prealbumin at 2.5 A resolution: A preliminary report on the polypeptide chain formation, quaternary structure and thyroxine binding, J. Mol. Biol. 88: 1–12 (1974).CrossRefGoogle Scholar
  7. 7.
    Fex. G., and Lindgren, R., Purification of a bovine counterpart to human prealbumin and its interaction with a bovine retinol-binding protein, Biochim. Biophys. Acta 433: 410–417 (1977).Google Scholar
  8. 8.
    Kopelman, M., Cogan, U., Mokady, S., and Shinitzky, M., The interaction between retinol-binding proteins and prealbumins studied by fluorescence polarization, Biochim. Biophys. Acta, 433: 449–460 (1976).Google Scholar
  9. 9.
    Rossi, T., Hirvonen, T., and Toivanen, P., The concentration of prealbumin in human fetal and infant sera, Scand. J. Clin. Lab. Invest. 26: 35–36 (1970).CrossRefGoogle Scholar
  10. 10.
    Giorgio, N. A., Jr., and Tabachnick, M., Thyroxine-protein interactions. V. Isolation and characterization of a thyroxin-binding globulin from human plasma, J. Biol. Chem. 243: 2247–2259 (1968).Google Scholar
  11. 11.
    Van der Walt, B. J., and Jaarsveld, P. P., Bovine thyroxine binding globulin. Purification and comparison with human thyroxin binding globulin, Biochem. Biophys. Acta 535: 44–53 (1978).Google Scholar
  12. 12.
    Robbins, J., Inherited variations in thyroxine transport, Mt. Sinai J. Med. 40: 511–519 (1973).Google Scholar
  13. 13.
    Refetoff, S., Robin, N. I., and Alper, C. A., Study of four new kindreds with inherited thyroxine-binding globulin abnormalities: Possible mutations of a single gene locus. J. Clin. Invest. 51: 848–867 (1972).CrossRefGoogle Scholar
  14. 14.
    Shane, S. R., Seal, U. S., and Jones, J. E., X-chromosome linked inheritance of elevated thyroxine binding globulin in association with goitre, J. Clin. Endocrinal. Metab. 32: 587–594 (1971).CrossRefGoogle Scholar
  15. 15.
    Premachandra, B. N., Gossain, V. V., and Perlstein, I. B., Increased free thyroxine in a euthyroid patient with thyroxine-binding globulin deficiency, J. Clin. Endocrinol. Metab. 42: 309–318 (1976).CrossRefGoogle Scholar
  16. 16.
    Singer, F., Aiginger, P., Vormittag, W., Zolle, I., and Hofer, R., TBG deficiency in male euthyroid patient with hypothyroxinemia, Endocrinol. Exp. (Bratisl.), 10: 297–302 (1976).Google Scholar
  17. 17.
    Hansen, J. M., and Siersbaek-Nielsen, K., Quantitative determination of TBG in familial elevation of serum thyroxin binding capacity of TBG, J. Clin. Endocrinol. Metab. 35: 461–462 (1972).CrossRefGoogle Scholar
  18. 18.
    Robbins, J., Genetic control of thyroxine transport, in Plasma Protein Turnover, R. Bianchi et al., Eds., University Park Press, Baltimore (1976), pp. 323–330.Google Scholar
  19. 19.
    Konno, N., Serum thyrotropin response to thyrotropin-releasing hormone and free thyroid hormone indices in patients with familiar thyroxine-binding globulin deficiency, Endocrinol. (JP N) 23: 313–317 (1976).CrossRefGoogle Scholar
  20. 20.
    Yee, H. Y., Katz, E. S., and Jenest, E. S., A rapid semimicro method for determining protein bound iodine, Clin. Chem. 13: 220–226 (1967).Google Scholar
  21. 21.
    Marlow, H. W., Improvement in PBI method, Clin. Chem. 4: 510–512 (1958).Google Scholar
  22. 22.
    Kontaxis, N. E., and Pickering, D. E., A micro-method for the determination of butyl-alcohol extractable hormonal iodine in serum, J. Clin. Endocrinol. 18: 774–786 (1958).CrossRefGoogle Scholar
  23. 23.
    Murphy, B. P., The determination of thyroxine by competitive protein binding analysis employing an ion-exchange resin and radiothyroxine, J. Clin. Lab. Med. 66: 161–167 (1965).Google Scholar
  24. 24.
    Rubenstein, H., Butler, V., and Werner, S., Progressive decrease in serum triiodothyronine concentration with human aging: Radio-immunoassay following extraction of serum, J. Clin. Endocrinol. Metab. 37: 247–253 (1973).CrossRefGoogle Scholar
  25. 25.
    Larsen, P. R., Dockalova, J., Sipula, D., and Wu, F. M., Immunoassay of thyroxine in unextracted human serum, J. Clin. Endocrinol. Metabo. 37: 177–182 (1973).CrossRefGoogle Scholar
  26. 26.
    Pain, R. W., Simple modifications of three routine in vitro tests of thyroid function, Clin. Chem. 22: 1715–1719 (1976).Google Scholar
  27. 26a.
    Special TLR Report, Free T4: Clarifying and resolving a controversy, Ligand Review 1: 7–15 (1979).Google Scholar
  28. 26b.
    Lewis, M., Relationship between TBG concentration and % FT4 as estimated by dialysis and by polyacrylamide gel filtration, Clin. Chem. 25: 1181 (1979).Google Scholar
  29. 27.
    Hesch, R. D., and Evered, D. Radioimmunoassay of triiodothyronine in unextracted human serum, Br. Med. J. 1: 645–648 (1973).CrossRefGoogle Scholar
  30. 27a.
    Scottolini, A. C., and Bhagavan, N. V., The effective thyroxin ratio as an effective discriminant between the euthyroid and non-euthyroid states, Lab. Med. 10: 164–167 (1979).Google Scholar
  31. 28.
    Barragry, J. M., and Burr, W. A., Growth retardation and familial thyroxine-binding globulin deficiency, Br. Med. J. 2: 742 (1977).CrossRefGoogle Scholar
  32. 29.
    Horwitz, D. L., and Refetoff, S., Graves disease associated with familial deficiency of thyroxine-binding globulin. J. Clin. Endocrinol. Metab. 44: 242–247 (1977).CrossRefGoogle Scholar
  33. 30.
    Bastomsky, C. H., Kalloo, H., and Frenkel-Leith, D. B., A simple ligand-binding assay for thyroxine-binding globulin on reusable Sephadex columns, Clin. Chim. Acta 74: 51–57 (1977).CrossRefGoogle Scholar
  34. 31.
    Mulaisho, C., and Utiger, R. D., Serum thyroxine-binding globulin: Determination by competitive ligand-binding assay in thyroid disease and pregnancy, Acta Endocrinol. 35: 314–324 (1977).Google Scholar
  35. 32.
    Kogedal, B., and Kallberg, M., Determination of thyroxine-binding globulin in human serum by single radial immunodiffusion and radioimmunoassay, Clin. Chem. 23: 1694–1699 (1977).Google Scholar
  36. 33.
    Lambergs, S. W., Casparie, A. F., Miedema, K., Hennemann, G., and Hulsmans, H. A., Thyroxine binding globulin deficiency in a family with Type I hyperlipoproteinaemia, Clin. Endocrinol. 6: 197–206 (1977).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Samuel Natelson
    • 1
  • Ethan A. Natelson
    • 2
  1. 1.Department of Environmental Practice, College of Veterinary MedicineUniversity of TennesseeKnoxvilleUSA
  2. 2.University of Texas Medical School and St. Joseph HospitalHoustonUSA

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