Somatic Hybridization and Genetic Manipulation in Plants

  • Indra K. Vasil
  • Vimla Vasil
  • Derek W. R. White
  • Howard R. Berg
Part of the Nato Advanced Study Institutes Series book series (NSSA, volume 22)


Following the discovery of cytokinins and their role in plant development (Skoog and Miller, 1957), and the demonstration of the totipotency of plant cells (Vasil and Hildebrandt, 1965; Vasil and Vasil, 1972), plant regeneration has been achieved in vitro from a wide variety of plant species (Murashige, 1974; Vasil et al., 1978). Although plant regeneration from the most important groups of crop plants, namely the cereals and the legumes, still remains a serious problem, the ability to rapidly clone plants through tissue culture techniques fulfills an important prerequisite for the utilization of the novel methods of somatic hybridization and genetic manipulation of plants. These cloning procedures ensure that any changes effected through somatic hybridization or other parasexual methods in the information content of plant cells can be perpetuated without necessarily going through the sexual cycle where they might very well be eliminated.


Nitrogen Fixation Genetic Manipulation Somatic Hybridization Plant Tissue Culture Protoplast Fusion 
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Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • Indra K. Vasil
    • 1
  • Vimla Vasil
    • 1
  • Derek W. R. White
    • 1
  • Howard R. Berg
    • 1
  1. 1.University of FloridaGainesvilleUSA

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