Opioid Peptides as Modulators of Cyclic AMP Levels
One of the most clearly defined biochemical actions of the opiates is the receptor mediated inhibition of adenylate cyclase. Collier and Roy (1974) showed that cAMP accumulation in rat brain homogenates is inhibited by opiates, that the inhibition is blocked by the specific inhibitor, naloxone, and that the potencies of a series of opiate agonists as blockers of cAMP formation are similar to their potencies as analgesic agents. Soon thereafter, Sharma, et al, (1975a) found that the adenylate cyclase of neuroblastoma X glioma cell (NG108-15) membranes is in hibited by opiates in a similar, receptor mediated, fashion. The neuroblastoma X glioma hybrid cell NG108-15 homogenates provide an easily accessible source of opiate receptors (KLEE and NIRENBERG, 197 4) which remain coupled to adenylate cyclase more tenuously than those in brain homogenates seem to be. The NG108-15 system is therefore more easily reproducible (VAN INWAGEN, et al, 1975). In a parallel series of studies, Traber, et al, (1975a), using the same neuroblastoma X glioma hybrid cell line, have also shown that opiates decrease cellular cAMP levels.
KeywordsAdenylate Cyclase Opioid Peptide Adenylate Cyclase Activity Wheat Gluten Opioid Activity
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