Advertisement

An Electrical Birefringence Study of the Contribution of Permanent and Induced Dipole Moments to Acid-Soluble Collagen Polarization

  • J. C. Bernengo
  • B. Roux
  • D. Herbage

Abstract

A method is described using decay, reverse and sine wave birefringence experiments to measure the electrical parameters of acid soluble collagen molecules in solutions containing both monomeric and aggregated forms. Mathematical analysis of decay and reverse signals is performed according to classical limiting low fields equations, and values of permanent and induced dipole moments can be obtained for both molecular species. A confirmation of these results and an evaluation of molecular flexibilities is given by comparison with sine wave birefringence measurements at very low applied fields.

Keywords

Dipole Moment Sine Wave Induce Polarization Permanent Dipole Moment Induce Dipole Moment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Yoshioka K and O’Konski C T, Biopolymers, 4 (1966), 499–507.CrossRefGoogle Scholar
  2. 2.
    Khan L D and Witnauer L P, Biochim. Biophys. Acta, 243 (1971), 388–397.Google Scholar
  3. 3.
    Bernengo J C, Roux B and Herbage D, Biopolymers, 13 (1974), 641–647.CrossRefGoogle Scholar
  4. 4.
    Bernengo J C, Herbage D, Marion C and Roux B, Biochim. Biophys. Acta, 532 (1978), 305–314.Google Scholar
  5. 5.
    Miller A, in ‘Biochemistry of Collagen’ (Plenum Press ed.) (1976), p. 85–121.Google Scholar
  6. 6.
    Benoit H, Ann. Phys. (Paris), 6 (1951), 561–609.Google Scholar
  7. 7.
    Benoit H, J. Chim. Phys., 49 (1951), 517–522.Google Scholar
  8. 8.
    Tinoco I and Yamaoka K, J. Phys. Chem., 63 (1959), 423–427.CrossRefGoogle Scholar
  9. 9.
    Thurston G B and Bowling D I, J. of Coll. and Interface Sci., 30 (1969), 34–45.CrossRefGoogle Scholar
  10. 10.
    Piez K, Lewis M, Martin C and Gross J, Biochem. Biophys. Acta, 53 (1961), 596–598.CrossRefGoogle Scholar
  11. 11.
    Drake M P, Davidson P F, Bump S and Schmitt F O, Biochemistry, 5 (1966), 301–312.CrossRefGoogle Scholar
  12. 12.
    Green R W, Ang K P and Lam L C, Biochem. J., 54 (1953) 181–187.Google Scholar
  13. 13.
    Bernengo J C, Thesis, Lyon University (1970).Google Scholar
  14. 14.
    Bernengo J C, Roux B and Hanss M, Rev. Sci. Instr., 44 (8) (1973), 1083–1086.CrossRefGoogle Scholar
  15. 15.
    Roux B, Thesis, Lyon University, (1976).Google Scholar
  16. 16.
    Coles H J and Jennings B R, Biopolymers, 14 (1975), 2567–2575.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • J. C. Bernengo
    • 1
  • B. Roux
  • D. Herbage
    • 2
  1. 1.Laboratoire de BiophysiqueUniversité de NiceNice-CedexFrance
  2. 2.Laboratoire de Biochimie et de Chimie MacromoléculaireUniversité Lyon IVilleurbanneFrance

Personalised recommendations