Effects of Calcium and ATP on the Conformation of F-Actin in Vivo and in Vitro

  • M. Taniguchi


The dichroism spectrum of I-band in a glycerinated single fiber of muscle was very similar to that of an oriented F-actin filament in vitro. The negative dichroism of F-actin in the thin filament in vivo or in vitro was found to change with the addition of Ca ions, suggesting a conformational change of F-actin. In the absence of ATP, a small amount of bound H-meromyosin induced a remarkable decrease of the dichroism of F-actin. Upon the addition of Mg-ATP, the negative dichroism of the F-actin-H-meromyosin complex became larger than that of pure F-actin, suggesting that F-actin interacting with H-meromyosin assumes a different conformation from pure F-actin. A similar increase was also observed in the F-actin-tropomyosin-troponin-H-meromyosin complex. The maximum increase was greater under the condition that super-precipitation was delayed. The possible role of F-actin in muscle contraction is discussed.


Negative Peak Thin Filament Thick Filament Linear Dichroism Rabbit Skeletal Muscle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation used


heavy meromyosin






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Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • M. Taniguchi
    • 1
  1. 1.Department of Physics, Faculty of ScienceNagoya UniversityNagoyaJapan

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