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Determination of Rotational Diffusion Coefficients of the Bacteriophages T4B and T7 by Depolarized Dynamic Light Scattering. The Influence of Double Scattering

  • P. C. Hopman
  • G. Koopmans
  • J. Greve

Abstract

Methods to correct for double scattering effects in the determination of rotational diffusion coefficients by depolarized dynamic light scattering are discussed. Rotational diffusion coefficients obtained by this technique for the bacteriophages T4B and T7 are compared to the ones measured by electro optical methods. The results obtained by both methods agree. This indicates that for both T4B and T7 the orienting electric field used in the electric birefringence technique does not influence the conformation of the bacteriophages during the field-free decay.

Keywords

Correlation Function Dynamic Light Scattering Channel Number Translational Diffusion Coefficient Double Scattering 
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References

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    Berne B J and Pecora R, “Dynamic light scattering”, John Wiley & Sons, New York (1976) and references herein.Google Scholar
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    De Groot G, Greve J and Blok Joh., Biopolymers, 16 (1977) 639.CrossRefGoogle Scholar
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    Hopman P C, Koopmans G and Greve J, manuscript in preparation.Google Scholar
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    Koopmans G, Hopman P C and Greve J, accepted for publication J. of Phys. A.Google Scholar
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    Kelly H C, J. of Phys. A 6 (1973) 373.Google Scholar
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    Sorensen C M, Mockler R C and O’Sullivan W J, Phys. Rev. A, 14 (1976) 1520.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • P. C. Hopman
    • 1
  • G. Koopmans
    • 1
  • J. Greve
    • 1
  1. 1.Biophysics DepartmentPhysics Laboratory of the Vrije UniversiteitAmsterdamThe Netherlands

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