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Electro-Optical Properties of Nucleosomes and Nucleosomal DNA

  • B. Roux
  • C. Marion
  • J. C. Bernengo

Abstract

Rat liver soluble chromatin is digested by micrococcal nuclease. The birefringence of the seven fractions obtained from sucrose gradient is studied in 1 mM phosphate buffer pH 7.4, 0.2 mM EDTA. Electro-optical properties and relaxation times show a sharp transition when the number n of nucleosomes in the oligomer chain is greater than 6: the negative birefringence becomes positive and no signal is observed for the hexamer. The monomer relaxation time (0.6 µs) agrees with a prolate ellipsoid model of dimensions about 140 x 140 x 70 Å. The relaxation time varies also with n: it is a maximum for the dimer (1.8 µs)and decreases slightly up to the pentamer. These results may be interpreted in terms of a superstructure of nucleosome chains.

Keywords

Longe Relaxation Time Micrococcal Nuclease Oligomer Chain Negative Birefringence Birefringence Signal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • B. Roux
    • 1
  • C. Marion
    • 1
  • J. C. Bernengo
    • 1
  1. 1.Laboratoire de Chimie BiologiqueUniversité C. Bernard Lyon IVilleurbanneFrance

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