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Electro-Optic Measurement of γ-Ray Induced Damage in DNA

  • C. T. O’Konski
  • R. S. Farinato

Abstract

The effects on aqueous superhelical PM2 and linear calf thymus DNA’s due to γ-radiation from a 60Co source have been studied by pulsed electric birefringence relaxation techniques. In a nitrogen atmosphere the average field-free relaxation time, T, for calf thymus DNA first increased, then decreased as a function of dose in the range 0–13 kRad. A much more rapid decrease in T as a function of dose was determined in the presence of ambient oxygen. Significant differences were easily measurable at the lowest dose tried (~ 0.3 kRad). Solutions of superhelical PM2 DNA in the presence of ambient oxygen showed an asymptotic increase in T by a factor of about 2. This was predicted for supercoil relaxation from a previous electro-optic study of PM2 DNA with ethidium bromide. Significant changes in T were easily discernable at the lowest doses measured (~ 0.3 kRad), with a 50% effect at 0.5 kRad.

Keywords

Strand Break Double Strand Break Ambient Oxygen Electric Field Pulse Contour Length 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • C. T. O’Konski
    • 1
  • R. S. Farinato
    • 1
  1. 1.Department of ChemistryUniversity of CaliforniaBerkeleyUSA

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