Polarized Fluorescence in an Electric Field: Theoretical Calculation at Arbitrary Fields, Experimental Comparison with Other Electrooptical Effects, Saturation of the Induced Dipole Moment in Polyelectrolytes
The molecular properties accessible from electrooptic effects are stressed and polarized fluorescence in an electric field is then introduced in view of its specific properties. The need to calculate the fourth moment of the orientation function at high fields is emphasized for its use in the determination of the mechanism of orientation. The results of such a calculation for permanent and induced dipole orientation are given. They are applied in an experimental comparison with electric birefringence and dichroism on fragments of DNA labelled with intercalated Acridine Orange. The significance of an apparent permanent dipole orientation mechanism is discussed in terms of saturation of the induced dipole moment in polyelectrolytes.
KeywordsAcridine Orange Orientation Function Polarize Fluorescence Orientation Factor Fourth Moment
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