Principles of Sterile Technique

  • Ervin H. Barnes


Bacteria and fungi are members of the microbiological world and are especially significant because of their universality. They are found everywhere: in the air, soil, hair, clothes, water, crevices in bark, paint, glue, crank-case oil, snow, and pickle juice. These little creatures also provide interesting paradoxes: (1) There are places where they are not normally found: the blood of healthy animals, the internal tissues of healthy plants and animals, and most conspicuous of all, sterilized objects. (2) Some are so undemanding in nutritional requirements as to survive and grow in stale distilled water containing traces of dust; others are demanding obligate parasites which require a certain cultivar of a particular species as host. (3) Some, like intestinal flora, are necessary whereas others, such as the tubercle bacillus and the Dutch elm fungus, cause severe and sometimes fatal diseases. (4) Some are red; others are yellow or white. (5) Some are uncommon while others are everywhere.


Serratia Marcescens Sterile Technique Spontaneous Generation Obligate Parasite Cotton Plug 
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Suggested Reading

  1. Difco Manual of Dehydrated Culture Media and Reagents. Detroit, Mich., Difco Laboratories, Inc., 1964.Google Scholar
  2. Riker, A. J., and Riker, Regina S., Introduction to Research on Plant Diseases. Chicago, John S. Swift Co., Inc., 1936.Google Scholar

Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • Ervin H. Barnes
    • 1
  1. 1.Michigan State UniversityUSA

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