Effect of Removal of Carbohydrate Residues upon the Half Life and in Vivo Biological Activity of Human Chorionic Gondadotropin

  • Satish K. Batta
  • M. A. Rabovsky
  • C. P. Channing
  • O. P. Bahl
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 112)


It has been previously shown (1, 2) that progressive removal of sialic acid from human chorionic gonadotropin (hCG) decreases the half life, as well as the in vivo biologic activity of the gonadotropin. The effect of removing sugar residues internal to sialic acid upon the half life and biologic activity of hCG has not yet been examined. In vitro studies, using isolated porcine (3) and monkey (4) granulosa cells, indicate that the successive removal of sugar residues internal to sialic acid from native hCG lead to a diminution in ability to stimulate cyclic AMP accumulation and progesterone secretion. The ability to inhibit binding of iodinated hCG to porcine granulosa cells was not appreciably altered provided the hCG derivative was added prior to the tracer molecule. In both monkey and pig granulosa cells the hCG derivatives with sugar residues internal to sialic acid removed, acted as competitive inhibitors of hCG action. In order to examine whether hCG derivatives have biologic activity in vivo and whether they have the ability to inhibit LH and hCG action in vivo their possible interfering effect on ovulation in prepuberal female rats primed with pregnant mare serum gonadotropin was studied. In addition, the half life of the hCG derivatives was examined in adult female rats.


Half Life Sialic Acid Granulosa Cell Corpus Luteum Carbohydrate Residue 
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  1. 1).
    Van Hall, E.V., J.L. Vaitukaitis, and G.T. Ross, Endocrinology 89 (1971) 11.PubMedCrossRefGoogle Scholar
  2. 2).
    Tsuruhara, T., E.V. Van Hall, M.L. Dufau, and K.J. Catt, Endocrinology, 91 (1972) 463.PubMedCrossRefGoogle Scholar
  3. 3).
    Channing, C.P., C.N. Sakai, and 0.P. Bahl, Fed. Proc. 35 (1976) Ab. 3268.Google Scholar
  4. 4).
    Channing, C.P. and 0.P. Bahl, Biol. Reprod. 17 (1978) 707.CrossRefGoogle Scholar
  5. 5).
    Moyle, W., 0.P. Bahl, and L. Marz, J. Biol. Chem. 250 (1975) 9163.PubMedGoogle Scholar
  6. 6).
    Kammerman, S. and R.E. Canfield, Endocrinology 90 (1972) 384.PubMedCrossRefGoogle Scholar
  7. 7).
    Sakai, C.N., B. Engel, & C.P. Channing,Proc. Soc. Exp. Biol. Med. 155. (1977) 373.Google Scholar
  8. 8).
    Batta, S.K., F. Piva, and L. Martini, Adv. in Biosci. 9 (1972) 723.Google Scholar
  9. 9).
    Sasamoto, S., and T. Johke, Biol. Reprod. 13 (1975) 195.PubMedCrossRefGoogle Scholar
  10. 10).
    Channing, C.P., C.N. Sakai, and 0.P. Bahl, Endocrinology 1978 (in press).Google Scholar
  11. 11).
    Morell, A.G., R.A. Irvine, I. Sternlieb, I.H. Scheinberg and G.G. Ashwell, J. Biol. Chem. 243 (1968) 155.PubMedGoogle Scholar
  12. 12).
    Morell, A.G., G. Gregoriadis, I.H. Scheinberg, J. Hickman, and G.G. Ashwell, J. Biol. Chem. 246 (1971) 1461.PubMedGoogle Scholar
  13. 13).
    Lunenfeld, B. and A. Eshkol, Vitam. Horm. 25 (1967) 137.PubMedCrossRefGoogle Scholar
  14. 14).
    Channing, C.P. and S. Kammerman, Endocrinology 93 (1973) 1035.PubMedCrossRefGoogle Scholar
  15. 15).
    Mizejewski, G.J., W.H. Beierwaltes and J. Quinones, J. Nucl, Med. 13 (1972) 101.Google Scholar
  16. 16).
    Kammerman, S. and C.P. Channing, J. Clin. Endocrinol, Metab. 38 (1974) 463.CrossRefGoogle Scholar
  17. 17).
    Nilsson, C., T. Hillensjä and C. Ekholm, Acta Endocrinol. (Kbh) 86 (1977) 384.Google Scholar
  18. 18).
    Turgeon, J. and C.A. Barraclough, Endocrinology 92 (1973) 755.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • Satish K. Batta
    • 1
  • M. A. Rabovsky
    • 1
  • C. P. Channing
    • 1
  • O. P. Bahl
    • 1
    • 2
  1. 1.Department of PhysiologyUniv. of Maryland Med. Sch.BaltimoreUSA
  2. 2.Div. of Cell and Molecular BiologySUNYBuffaloUSA

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