FSH Stimulation of Mucopolysaccharide Synthesis in Rat and Porcine Ovary

  • R. L. Ax
  • A. R. LaBarbera
  • R. J. Ryan
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 112)

Abstract

Incorporation of mucopolysaccharides (MPS) into follicular fluid occurs during antrum formation in follicular development. Staining of follicular fluid with toluidine blue or autoradiographic localization of 35S showed that concentrations of MPS varied during the ovulatory cycle (3,9,10,11). FSH stimulated in vivo uptake of 35S into ovarian follicular MPS in hypophysectomized immature female rats and in vitro uptake of 35S by rat granulosa cells (8). Conversely, a decrease of in vitro incorporation of 35S by rat ovarian slices was evoked by either LH or progesterone (4), implying antagonistic functions of FSH and LH on MPS accumulation. The physiological significance of MPS on follicular and oocyte maturation and on ovulation remains to be resolved. This report confirms FSH modulation of incorporation of MPS into follicular fluid in vivo and in vitro and contains data showing quantitative changes in the concentrations of MPS in porcine follicular fluid.

Keywords

Hyaluronic Acid Granulosa Cell Chondroitin Sulfate High Pressure Liquid Chromatography Follicular Fluid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. (1).
    Ax, R. L. and R. J. Ryan. Manuscript in preparation.Google Scholar
  2. (2).
    Chang, S. C. S., J. D. Jones, R. D. Ellefson, and R. J. Ryan. Biol. Reprod. 15(1976)321.PubMedCrossRefGoogle Scholar
  3. (3).
    Dziewiatkowski, D. D. Int. Rev. Cyt. 7(1958)159.Google Scholar
  4. (4).
    Gebauer, H., H. R. Lindner, and A. Amsterdam. Biol. Reprod. 18(1978)350.PubMedCrossRefGoogle Scholar
  5. (5).
    Jensen, C. E. and F. Zachariae. Acta Endocr. 27(1958)356.PubMedGoogle Scholar
  6. (6).
    Kissane, J. M. and E. Robins. J. Biol. Chem. 233(1958)184.PubMedGoogle Scholar
  7. (7).
    Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. J. Biol. Chem. 193(1951)265.PubMedGoogle Scholar
  8. (8).
    Mueller, P. L., J. R. Schreiber, A. W. Lucky, J. D. Schulman, D. Rodbard, and G. T. Ross. Endocrinology 102(1978)824.Google Scholar
  9. (9).
    Oldeblad, E. Acta Endocr. 11(1952)269.Google Scholar
  10. (10).
    Oldeblad, E. Acta Endocr. 15(1954)313.Google Scholar
  11. (11).
    Zachariae, F. and C. E. Jensen. Acta Endocr. 27(1958)343.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • R. L. Ax
    • 1
  • A. R. LaBarbera
    • 1
  • R. J. Ryan
    • 1
  1. 1.Mayo Clinic and FoundationRochesterUSA

Personalised recommendations