Demonstration of Luteal Cell Membrane Receptors for Prostaglandin F2α by Ultrastructural and Binding Analysis
Prostaglandins have a ubiquitous distribution and may act on a variety of cell types (1). To date, there have been no studies in which prostaglandin binding sites were directly localized by morphological methods. Prostaglandin F2α (PGF2α) has been shown to act directly on isolated rat luteal cells to block luteinizing hormone action (2) and is thought to be involved in luteolysis in vivo (3,4). However, specific binding sites for PGF2α have not been demonstrated in rat luteal tissue. The purpose of this study was to determine the distribution and specificity of PGF2α binding sites on cells and membranes isolated from rat luteal tissue by ultrastructural and binding analysis respectively.
KeywordsLuteal Cell Binding Analysis Ferritin Molecule Corpus Luteum Function Luteinizing Hormone Action
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