Effects of Cholera Toxin, LH and PGF2α on Steroidogenesis in Luteal Cells
Recent studies using cultured rat luteal cells (1) showed that PGF2α inhibits accumulation of cyclic AMP (cAMP) and progesterone secretion in response to LH, while having little effect on the binding of LH to its receptor. This inhibition of progesterone secretion was reversed by simultaneous addition of exogenous cyclic AMP but not by theophylline (1). It was concluded that PGF2α prevents accumulation of cAMP stimulated by the LH-receptor complex. Secondary factors may mediate the action of PGF2α in intact cells since it does not inhibit LH-stimulated adenylate cyclase activity in isolated luteal membranes (2).
KeywordsAdenylate Cyclase Corpus Luteum Cholera Toxin Luteal Cell cAMP Accumulation
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