Modulation of Pituitary Responsiveness to LHRH by Porcine Follicular Fluid: Time and dose- Dependent Effects
The injection of porcine follicular fluid (pFF1) can suppress both the rise in serum FSH that occurs in rats after ovariectomy (1) and FSH surges that occur in rats during proestrus (1600 h and 1900 h) and estrus (0800 h and 1100h)(2). Furthermore, it has been shown that pFF1 inhibits FSH secretion after LHRH infusion in phenobarbital-treated, proestrus rats (2), suggesting that pFFl exerts its suppressive effects at the level of the pituitary gland. Previously, it has been shown in vitro, using monolayers of pituitary cells, that non-steroidal substances of gonadal origin can directly inhibit gonadotropin responses to LHRH (3). Thus, the addition of rete testes fluid (free of steroids) to pituitary cells in a monolayer culture system resulted in an inhibition of both LH and FSH secretion in response to LHRH. The above findings prompted the present investigation which was designed to study the effects of charcoal-treated pFF1 on both basal and LHRH-stimulated secretion of FSH and LH from rat anterior pituitary cells cultured in monolayer.
KeywordsGranulosa Cell Pituitary Cell Antral Follicle Dulbecco Modify Eagle Medium Porcine Granulosa Cell
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