Inhibition of in Vivo and in Vitro FSH Secretion by Steroid-Free Hamster Ovarian Extracts

  • S. C. Chappel
  • T. Acott
  • H. G. Spies
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 112)


We designed experiments to determine if a nonsteroidal substance capable of inhibiting the secretion of follicle-stimulating hormone (FSH) exists in the hamster ovary. Proestrous hamster ovaries were homogenized, centrifuged, and treated with dextrancoated charcoal for removal of steroids. Charcoal-treated ovarian extract (CTOE) was injected intraperitoneally at hourly intervals into phenobarbital-treated (phen-treated) proestrous hamsters immediately after ovariectomy at 1300. The CTOE inhibited the selective rise in serum FSH that occurred in phen-treated, ovariectomized (ovx) controls.


Luteinizing Hormone Follicular Fluid Pituitary Cell Luteinizing Hormone Level Anterior Pituitary Cell 
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Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • S. C. Chappel
    • 1
  • T. Acott
    • 1
  • H. G. Spies
    • 1
  1. 1.Oregon Regional Primate Research CenterReproductive PhysiologyBeavertonUSA

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