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Gonadal LH Receptors: Resolution from Adenylate Cyclase and Transfer to Heterologous Cells

  • M. L. Dufau
  • K. Hayashi
  • G. Sala
  • A. Baukal
  • K. J. Catt
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 112)

Abstract

The majority of particulate high-affinity receptors for LH in the steroidogenic cells of the testis and ovary are membrane-bound and capable of functional coupling to adenylate cyclase(1,2). The gonadal receptors for LH can be solubilized together with adenylate cyclase by a variety of non-ionic detergents(3). In addition to the membrane-associated receptors that require detergent for solubilization, the luteinized ovary has been found to contain a small proportion of spontaneously “soluble” receptor sites (up to 10%) that can be recovered in the floating lipid fraction of the 360,000 x g supernate prepared from ovarian homogenates (4).

Keywords

Adenylate Cyclase DEAE Cellulose Adenylate Cyclase Activity Adrenal Cell Steroidogenic Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • M. L. Dufau
    • 1
  • K. Hayashi
    • 1
  • G. Sala
    • 1
  • A. Baukal
    • 1
  • K. J. Catt
    • 1
  1. 1.Endocrinology and Reproduction Research BranchNICHD National Institutes of HealthBethesdaUSA

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