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Long Term Perifusion of Rabbit Ovarian Follicles

  • C. D’Amato
  • J. Bahr
  • B. Stockert
  • F. Calvo
  • D. Kesler
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 112)

Abstract

Preovulatory follicles were dissected from ovaries of estrous rabbits. Four follicles were placed in each chamber of a perifusion system. Medium 199, containing 10% rabbit serum and antibiotics, eluted through the chambers at a rate of 1 ml/10 min. Every 6 h for 36 h, 1 µg of LH and 50 ng of FSH were injected into each chamber. P4, T and E2 were measured in the medium. P4 exhibited a peak after each of the injections at 6, 12, 18, 24 and 30 h. E2 and T gave major peaks after the injection at 0 time, with minor peaks after the injection at 6 h; thereafter T and E2 secretion gradually decreased to levels below those of baseline.

Keywords

Granulosa Cell Preovulatory Follicle Steroid Secretion Adenylyl Cyclase System Corpus Luteum Function 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • C. D’Amato
    • 1
  • J. Bahr
    • 1
  • B. Stockert
    • 1
  • F. Calvo
    • 1
  • D. Kesler
    • 1
  1. 1.Departments of Animal Science and Physiology, Animal Genetics LaboratoryUniversity of IllinoisUrbanaUSA

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