Hormone Requirements for Long-Term Maintenance of Rat Granulosa Cell Cultures

  • Grace M. Centola
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 112)


Since the pioneering work of Astwood (1), prolactin has been considered the major luteotrophic agent in the rat. Maintenance of rat luteal function in vitro beyond 6–8 days has been difficult (2,3). The relationship between luteal function and the role played by the other pituitary gonadotrophins, steroids and placental hormones is not entirely understood. This is further complicated by the apparent fluctuations in the levels of the luteotrophic hormones required for in vivo maintenance of luteal function (6, 7, 10, 11). The present study was undertaken to determine the hormone requirements for luteinization and long-term maintenance of rat granulosa cell cultures. An attempt was made to reconcile the apparent conflicting results from in vivo and in vitro maintenance of luteal function.


Granulosa Cell Pituitary Gonadotrophin Luteal Function Progesterone Secretion Culture Figure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. (1).
    E.B. Astwood. Endocrinology 28(1941)309.Google Scholar
  2. (2).
    T.M. Crisp and F.R. Denys, in: Electron Microscopic Concepts of Secretion: Ultrasctructure of Endocrine and Reproductive Organs, ed. M. Hess ( John Wiley and Sons, Inc., New York, 1975 ) pp. 3–33.Google Scholar
  3. (3).
    T.M. Crisp. Endocrinology 101 (1977) 1286.Google Scholar
  4. (4).
    C.P. Channing. Endocrinology 94 (1974) 1215.Google Scholar
  5. (5).
    N.D. Goldberg, R.F. O’Dea and M.K. Haddox. Adv. in Nucleotide Res. 3(1973)155.Google Scholar
  6. (6).
    M. Takayama and G.S. Greenwald. J. Endoc. 56(1973)421.CrossRefGoogle Scholar
  7. (7).
    W.K. Morishige and I. Rothchild. Endocrinology 95(1974)260.PubMedCrossRefGoogle Scholar
  8. (8).
    A.W. Lucky, J.R. Schreiber, S.G. Hillier, J.D. Schulman and G.T. Ross. Endocrinology 100(1977)128.Google Scholar
  9. (9).
    B.J.A. Furr. Acta Endocrinol. Kbh 72(1973)89.Google Scholar
  10. (10).
    I. Rothchild, G.J. Pepe, and W.K. Morishige. Endocrinology 95(1974)280.Google Scholar
  11. (11).
    H.G. Madhwa Raj and N.R. Moudgal. Endocrinology 86(1970)874.Google Scholar
  12. (12).
    C.P. Channing. Endocrinology 87(1970)49.Google Scholar
  13. (13).
    C.P. Channing. Rec. Prog. Horm. Res. 26(1970)589.Google Scholar
  14. (14).
    J.S. Richards. Endocrinology 96(1975)227.Google Scholar
  15. (15).
    G.E. Gibori, E. Antczak and I. Rothchild. Endocrinology 100 (1977) 1483.Google Scholar
  16. (16).
    W.K. Morishige, G.J. Pepe and I. Rothchild. Endocrinology 92 (1973) 1527.Google Scholar
  17. (17).
    C.P. Channing and S. Kammerman. Endocrinology 92(1973)531.Google Scholar
  18. (18).
    C.P. Channing and S. Kammerman. Biol. Reprod. 10(1974)179.CrossRefGoogle Scholar
  19. (19).
    R.J. Ryan and C.Y. Lee. Biol. Reprod. 14(1976)16.PubMedCrossRefGoogle Scholar
  20. (20).
    D.T. Armstrong and J.H. Dorrington. Endocrinology 99 (1976) 1411.Google Scholar
  21. (21).
    J. Bernard. J. Reprod. Fertil. 43(1975)453.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • Grace M. Centola
    • 1
  1. 1.Department of AnatomyUniversity of Maryland Dental SchoolBaltimoreUSA

Personalised recommendations