Hormone Requirements for Long-Term Maintenance of Rat Granulosa Cell Cultures

  • Grace M. Centola
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 112)

Abstract

Since the pioneering work of Astwood (1), prolactin has been considered the major luteotrophic agent in the rat. Maintenance of rat luteal function in vitro beyond 6–8 days has been difficult (2,3). The relationship between luteal function and the role played by the other pituitary gonadotrophins, steroids and placental hormones is not entirely understood. This is further complicated by the apparent fluctuations in the levels of the luteotrophic hormones required for in vivo maintenance of luteal function (6, 7, 10, 11). The present study was undertaken to determine the hormone requirements for luteinization and long-term maintenance of rat granulosa cell cultures. An attempt was made to reconcile the apparent conflicting results from in vivo and in vitro maintenance of luteal function.

Keywords

Testosterone Progesterone Androgen Estradiol Prolactin 

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Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • Grace M. Centola
    • 1
  1. 1.Department of AnatomyUniversity of Maryland Dental SchoolBaltimoreUSA

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