Advertisement

Testosterone Synthesis by Chicken Follicular Cells

  • Eileen Su-Rong Huang
  • A. V. Nalbandov
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 112)

Abstract

The roles of theca and of granulosa cells in the synthesis of testosterone (T) in ovarian follicle of hens have been studied. Theca cells alone synthesized no progesterone (P), and no or only negligible amounts of T. Granulosa cells, in contrast, without stimulation produced large amounts of P and small amounts of T. When incubated with ovine gonadotrophins, theca cells showed greater response than granulosa cells in synthesizing more T (2- to 2.5-fold increase). Combination of the two follicular cell types decreased synthesis of P, but increased that of T (9- to 17-fold). When 0.1 pg of ovine luteinizing hormone (oLH) was added to the combined cells, T concentrations increased most dramatically, a 83-fold increase was seen after 12 h of incubation. Theca cells incubated with exogenous P also produced large amounts of T. These results indicated that in hens, theca cells may be the major site of follicular T synthesis, however, theca cells alone cannot synthesize T unless granulosa cells or P were added. It is suggested that granulosa cells may use P as a precursor, and that P is converted to T in the theca cells.

Keywords

Granulosa Cell Ovarian Follicle Follicular Cell Theca Cell Large Follicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. (1).
    A. Makris and K. J. Ryan. Endocrinology 96 (1975) 694.PubMedCrossRefGoogle Scholar
  2. (2).
    G. F. Erickson and K. J. Ryan. Endocrinology 99 (1976) 452.PubMedCrossRefGoogle Scholar
  3. (3).
    J. E. Fortune and D. T. Armstrong. Endocrinology 100 (1977) 1341.PubMedCrossRefGoogle Scholar
  4. (4).
    N. A. Shahabi, H. W. Norton and A. V. Nalbandov. Endocrinology 96 (1975) 962.PubMedCrossRefGoogle Scholar
  5. (5).
    J. E. Fortune and D. T. Armstrong. Endocrinology 102 (1978) 227.PubMedCrossRefGoogle Scholar
  6. (6).
    G. M. Syburn and A. H. Baillie. in: Physiology of the Domestic Fowl, eds. C. Horton-Smith and E. C. Amoroso ( Oliver and Boyd, Edinburgh and London, 1966 ) P. 30.Google Scholar
  7. (7).
    E. S. Huang. In Vitro Steroidogenesis by Ovarian Follicular Cells of Hens. Ph.D. thesis. (University of Illinois, Urbana, Illinois, 1978 ).Google Scholar

Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • Eileen Su-Rong Huang
    • 1
  • A. V. Nalbandov
    • 1
  1. 1.Animal Genetics LaboratoryUniversity of IllinoisUrbanaUSA

Personalised recommendations