Steroidal Modulation of Steroid Secretion in vitro: An Experimental Approach to Intra-Follicular Regulatory Mechanisms

  • David W. Schomberg
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 112)


The secretion of steroid hormones by the pre-ovulatory ovarian follicle is a complex developmental process which integrates several modalities of endocrine regulation. In addition to the well-recognized participation of two cell types in steroid secretion (1,2), both of which are gonadotropin-dependent (3), it is now becoming apparent that follicular steroidogenesis is also modulated by the steroid hormones themselves. Based primarily upon studies in vitro, two types of modulation have been demonstrated to date: 1) a substrate model in which the conversion of androgen to estrogen is a rate-limiting step dependent upon follicle stimulating hormone (FSH), and 2) a regulatory model in which direct stimulatory or inhibitory steroidal action alters steroid secretion via mechanisims not yet understood. In this chapter a brief review of the studies which formed these lines of research will be presented followed by a discussion of on-going work in our laboratory which addresses certain aspects of both models. The foci of these studies involve: 1) developmental considerations in granulosa cell estrogen biosynthesis, 2) examination of the effects of estradiol (E2) and dihydrostestosterone (DHT) upon progesterone secretion in vitro by highly differentiated porcine granulosa cells from large follicles, 3) characterization of the effect of intra-ovarian implants of anti-androgen (AA) upon subsequent granulosa cell progesterone secretion in vitro, and 4) preliminary investigation of the possible mechanism(s) of DHT-stimulated progesterone production in vitro.


Granulosa Cell Follicle Stimulate Hormone Follicular Fluid Estrogen Production Follicle Development 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. (1).
    R.V. Short. J. Endocr. 24 (1962) 59.PubMedCrossRefGoogle Scholar
  2. (2).
    D.T. Armstrong and J.H. Dorrington, in: Regulatory Mechanisms Affecting Gonadal Hormone Action, Advances in Six Hormone Research, Vol. 3, eds. J.A. Thomas and R.L. Singhal ( University Park Press, Baltimore, 1977 ) p. 217.Google Scholar
  3. (3).
    G.S. Greenwald, in: Handbook of Physiology, Section 7, Endocrinology, Vol. IV, ed. R.O. Greep (American Physiological Society, Washington, D.C., 1974 ) p. 193.Google Scholar
  4. (4).
    K. Savard, J.M. Marsh, and B.F. Rice. Rec. Progr. Hormone Res. 21(1965)285.Google Scholar
  5. (5).
    B. Falck. Acta Physiol. Scand. (Suppl. 163) 47(1959)1.CrossRefGoogle Scholar
  6. (6).
    K.J. Ryan and K. Petro. J. Clin. Endo. Metab. 28(1968)355.CrossRefGoogle Scholar
  7. (7).
    L. Bjersing and H. Carstensen. Biochim. Biophys. Acta 86(1964)639.PubMedCrossRefGoogle Scholar
  8. (8).
    Y.S. Moon, J.H. Dorrington, and D.T. Armstrong. Endocrinology 97(1975)244.PubMedCrossRefGoogle Scholar
  9. (9).
    J.H. Dorrington, Y.S. Moon, and D.T. Armstrong. Endocrinology 97 (1975) 1328.PubMedCrossRefGoogle Scholar
  10. (10).
    J.E. Fortune and D.T. Armstrong. Endocrinology. 100 (1977) 1341.PubMedCrossRefGoogle Scholar
  11. (11).
    J.E. Fortune and D.T. Armstrong, in: Proceedings of the NIH Workshop on Ovarian Follicular Development and Function, Santa Cruz, Calif., 1977 ( In press).Google Scholar
  12. (12).
    G.F. Erickson and A.J.W. Hsueh. Endocrinology 102 (1978) 1275.PubMedCrossRefGoogle Scholar
  13. (13).
    E.V. Younglai and R.V. Short. J. Endocrinol. 47(1970)321.PubMedCrossRefGoogle Scholar
  14. (14).
    C.P. Channing and S.P. Coudert. Endocrinology 98(1976)590.PubMedCrossRefGoogle Scholar
  15. (15).
    H.R. Lindner, A. Tsafriri, M.E. Lieberman, U. Zor, Y. Koch, S. Bauminger, and A. Bornea. Rec. Progr. Hormone Res. 30(1974)79.Google Scholar
  16. (16).
    R.M. Moor. J. Endocr. 61(1974)455.PubMedCrossRefGoogle Scholar
  17. (17).
    J.S.G. Biggs, T.G. Baker, and D. Hutchon. Brit. J. Obstet. Gynec. 85(1978)211.CrossRefGoogle Scholar
  18. (18).
    K.J. Ryan and R.V. Short. Endocrinology 76(1965)108.PubMedCrossRefGoogle Scholar
  19. (19).
    E.W. Lacroix, W. Eeuchaute, and I. Leusen. Steroids 23 (1974) 337.PubMedCrossRefGoogle Scholar
  20. (20).
    A. Makris and K.J. Ryan. Steroids 29(1977)65.PubMedCrossRefGoogle Scholar
  21. (21).
    R.M. Moor. J. Endocr. 73(1977)143.PubMedCrossRefGoogle Scholar
  22. (22).
    J.E. Fortune and D.T. Armstrong. Endocrinology 102(1978)227.PubMedCrossRefGoogle Scholar
  23. (23).
    S. Aksel, D.W. Schoenberg, L. Tyrey, and C.B. Hammond. Am. J. Obstet. Gynecol. 126(1976)165.Google Scholar
  24. (24).
    S.S.C. Chang, J.D. Jones, R.D. Ellefson, and R.J. Ryan. Biol. Reprod. 15(1976)321.Google Scholar
  25. (25).
    C.R. Parker, Jr., A. Costoff, T.G. Muldoon, and V.B. Mahesh. Endocrinology 98(1976)129.Google Scholar
  26. (26).
    Y. Katz and D.T. Armstrong. Endocrinology 99 (1976) 1442.PubMedCrossRefGoogle Scholar
  27. (27).
    R.L. Stouffer, L. Tyrey, and D.W. Schomberg. Endocrinology 99(1976)516.Google Scholar
  28. (28).
    D.W. Schomberg, R.L. Stouffer, and L. Tyrey. Biochem. Biophys. Res. Commun. 68(1976)77.PubMedCrossRefGoogle Scholar
  29. (29).
    H.R. Lindner, A. Amsterdam, Y. Salomon, A. Tsafriri, A. Nimrod, S.A. Lamprecht, U. Zor, and Y. Koch. J. Reprod. Fert. 51(1977)215.Google Scholar
  30. (30).
    I.F. Stein and M.L. Leventhal. Am. J. Obstet. Gynec. 29 (1935) 181.Google Scholar
  31. (31).
    J.H. Gaarenstroom and S.E. De Jongh. A Contribution to the Knowledge of the Influences of Gonadotropic and Sex Hormones on the Gonads of Rats ( Elsevier, New York, 1946 ) p. 87.Google Scholar
  32. (32).
    R.W. Payne and R.H. Runsen. Endocrinology 62(1958)313.Google Scholar
  33. (33).
    R.M. Moor, M.F. May, and R.F. Seamark. J. Reprod. Fert. 45(1975)595.CrossRefGoogle Scholar
  34. (34).
    A. Nimrod and H.R. Lindner. Mol. Cell. Endocrinol. 5(1976)315.Google Scholar
  35. (35).
    D.T. Armstrong and J.H. Dorrington. Endocrinology 99 (1976) 1411.PubMedCrossRefGoogle Scholar
  36. (36).
    A.W. Lucky, J.R. Schreiber, S.G. Hillier, J.D. Schulman, and G.T. Ross. Endocrinology 100(1977)128.Google Scholar
  37. (37).
    M. Shemesh and M. Ailenberg. Biol. Reprod. 17(1977)499.PubMedCrossRefGoogle Scholar
  38. (38).
    S. Saiduddin and H.P. Zassenhaus. Endocrinology 102 (1978) 1069.PubMedCrossRefGoogle Scholar
  39. (39).
    S.G. Hillier, R.A. Knazek, and G.T. Ross. Endocrinology 100 (1977) 1539.Google Scholar
  40. (40).
    D.W. Schomberg, R.F. Williams, L. Tyrey, and L.C. Ulberg. Endocrinology 102(1978)984.PubMedCrossRefGoogle Scholar
  41. (41).
    T. Mori, A. Suzuki, T. Nishimura, and A. Kombegawa. Endocrinology 101(1977)623.Google Scholar
  42. (42).
    J.R. Schreiber and G.T. Ross. Endocrinology 99(1976)590.PubMedGoogle Scholar
  43. (43).
    S. Strickland and W.H. Beers. J. Biol. Chem. 251(1976)5694.Google Scholar
  44. (44).
    J.P. Louvet, S.M. Harman, J.R. Schreiber, and G.T. Ross. Endocrinology 97(1975)366.Google Scholar
  45. (45).
    J.S. Richards and A.R. Midgley, Jr. Biol. Reprod. 14 (1976) 82.CrossRefGoogle Scholar
  46. (46).
    A. Nimrod. Mol. Cell. Endocrinol. 8(1977a)189.PubMedCrossRefGoogle Scholar
  47. (47).
    A. Nimrod. Mol. Cell. Endocrinol. 8(1977b)201.PubMedCrossRefGoogle Scholar
  48. (48).
    M.T. Williams, M.R. Clark, and J.M. Marsh. Proc. 59th Ann. Meeting Endocrine Soc. Abstr. 231(1977)172.Google Scholar
  49. (49).
    P. Greengard. Science 199(1978)46.CrossRefGoogle Scholar
  50. (50).
    J.M. David, C.V. Byers, and D.H. Russell. Proc. Natl. Acad. Sci. USA 75(1978)223.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • David W. Schomberg
    • 1
  1. 1.Departments of Obstetrics and Gynecology and PhysiologyDuke University Medical CenterDurhamUSA

Personalised recommendations