Effect of the Administration of an Ovulatory Dose of HCG on the Subcellular Distribution of Follicular Protein Kinase
Most protein hormones initate a biological response in their target cells by initially binding to a membrane-localized receptor, thereby activating adenylyl cyclase and raising intracellular levels of cAMP. Currently, the only known manner in which cAMP can mediate cellular function is through cAMP-dependent protein kinases (PK). cAMP has been shown to activate cAMP-dependent PK by binding to the regulatory subunit of the inactive holoenzyme, thereby releasing the active, cAMP-insensitive catalytic subunit which can catalyze the transfer of phosphate form ATP to cellular protein (1). Presumably, the phosphorylation of specific cellular protein(s) regulates cellular activity in such a way that the known hormonal response ensues.
KeywordsProtein Kinase Activity Ovarian Follicle Postovulatory Follicle Graafian Follicle Protein Kinase Assay
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