LH-Stimulated cAMP Formation in Rat Granulosa Cells During Follicular Maturation — A Non-Refractory Response

  • L. Hamberger
  • L. Nilsson
  • K. Nordenström
  • A. Sjögren
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 112)


In early experiments on isolated granulosa cells from porcine ovaries Kolena and Channing (1,2) reported that the in vitro administration of either FSH or LH caused an acute stimulation of cAMP formation by these cells. The same group (3) also found that there was an induction of LH receptors on the surface of granulosa cells that parallelled with follicular maturation. Both FSH and estradiol are believed to be essential for the development of LH receptors in rat granulosa cells (4, 5, 6). In recent studies on granulosa cells from immature rat ovaries (7, 8) and on granulosa cells from small follicles isolated from sheep ovaries (9), LH was found to have a lack of effect in vitro on cyclic AMP formation. With increasing maturation of follicles from which the granulosa cells were derived, LH was not, in the case of the rat, able to stimulate cyclic AMP formation until the preovulatory stage. Exposure to LH in vitro of intact preovulatory follicles from this species caused acute stimulation of cAMP formation (10) but in addition refractoriness to a new stimulation by the same hormone is induced for several hours (11). A similar mechanism seems to be active in vivo with a decreased sensitivity to LH after the endogenous LH-FSH surge (12, 13). In general, the mechanism(s) for hormonal refractoriness is poorly understood and has in the above-mentioned context not been investigated in detail (14). The present study was intended to explore if a similar refractoriness to LH is present in granulosa cells separated and isolated from preovulatory rat follicles obtained from a PMSG rat model.


Granulosa Cell Preovulatory Follicle cAMP Formation Follicular Maturation Acute Stimulation 
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  1. (1).
    J. Kolena and C. P. Channing. Biochim. Biophys. Acta 252 (1971) 601.PubMedCrossRefGoogle Scholar
  2. (2).
    J. Kolena and C. P. Channing. Endocrinology 90 (1972) 1543.Google Scholar
  3. (3).
    C. P. Channing and S. Kammerman. Endocrinology 92 (1973) 531.PubMedCrossRefGoogle Scholar
  4. (4).
    A. J. Zelesnik, A. R. Jr. Midgley and L. E. Jr. Reichert. Endocrinology 95 (1974) 818.CrossRefGoogle Scholar
  5. (5).
    J. S. Richards. Endocrinology 97 (1975) 1174.PubMedCrossRefGoogle Scholar
  6. (6).
    A. Nimrod, E. Bedrak and S. A. Lamprecht. Biochim. Biophys. Res. Commun. 78 (1977) 977.CrossRefGoogle Scholar
  7. (7).
    A. K. Goff and D. T. Armstrong. Endocrinology 101 (1977) 1461.PubMedCrossRefGoogle Scholar
  8. (8).
    L. Hamberger, K. Nordenström, S. Rosberg and A. Sjögren. Acta Endocrinologica (1978) in press.Google Scholar
  9. (9).
    T. J. Weiss and D. T. Armstrong, ASRB meeting, Australia (1977).Google Scholar
  10. (10).
    L. Nilsson, S. Rosberg and K. Ahrén. Acta Endocrinologica (Kbh.) 77 (1974) 559.Google Scholar
  11. (11).
    S. A. Lamprecht, U. Zor, A. Tsafriri and H. R. Lindner. J. Endocrinol. 57 (1973) 217.PubMedCrossRefGoogle Scholar
  12. (12).
    M. Hunzicker-Dunn and L. Birnbaumer. Endocrinology 99 (1976) 211.PubMedCrossRefGoogle Scholar
  13. (13).
    L. Nilsson, T. Hillensjö and C. Ekholm. Acta Endocrinologica (Kbh.) 86 (1977a) 384.Google Scholar
  14. (14).
    S. A. Lamprecht, U. Zor, Y. Salomon, Y. Koch, K. Ahrén and H. R. Lindner. J. Cyclic Nucleotide Research 3 (1977) 69.Google Scholar
  15. (15).
    H. Herlitz, Y. Koch, M. I. Khan and K. Ahrén. Eur. J. Obstet. Gyn. Reprod. Biol. 6 (1976) 175.CrossRefGoogle Scholar
  16. (16).
    O. H. Lowry, N. J. Rosebrough, A. L. Farr and R. J. Randall. J. Biol. Chem. 193 (1951) 265.PubMedGoogle Scholar
  17. (17).
    A. G. Gilman. Proc. Natl. Acad. Sci. (USA) 67 (1970) 305.CrossRefGoogle Scholar
  18. (18).
    J. M. Marsh, T. M. Mills and W. J. Lemaire. Biochim. Biophys Acta 304 (1973) 197.PubMedCrossRefGoogle Scholar
  19. (19).
    L. Nilsson, L. Hamberger, T. Hillensjö and C. Ekholm. Acta Endocrinologica (Kbh.) 85 (1977b) 39.Google Scholar
  20. (20).
    U. Zor, S. A. Lamprecht, Z. Misulovin, Y. Koch and H. R. Lindner. Biochim. Biophys. Acta 428 (1976) 761.PubMedCrossRefGoogle Scholar
  21. (21).
    M. Conti, J. P. Harwood, A. J. W. Hsueh, M. L. Dufau and K. K. J. Catt. J. Biol. Chem. 251 (1976) 7729.PubMedGoogle Scholar
  22. (22).
    C. Magnusson and T. Hillensjö. J. exp. Zool. 201 (1977) 139.PubMedCrossRefGoogle Scholar
  23. (23).
    T. Hillensjö, C. Ekholm and K. Ahrén. Acta Endocrinologica 87 (1978) 377.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • L. Hamberger
    • 1
  • L. Nilsson
    • 1
  • K. Nordenström
    • 1
  • A. Sjögren
    • 1
  1. 1.Dept. of PhysiologyUniversity of GöteborgSweden

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