Biochemical and Biological Problems and Pitfalls of Cell Culture for Prenatal Diagnosis
The analysis of cultivated amniotic fluid cells has proved to be the most reliable tool for the prenatal diagnosis of chromosomal abnormalities and inborn errors of metabolism. It is of paramount importance that extreme caution be exercised in the interpretation of results obtained from the analysis of these cells. In many instances, a decision as to whether or not a pregnancy will be continued is based on the result of a single laboratory study. The responsibility entrusted to those involved in prenatal diagnostic studies is therefore great. It is essential that everyone involved in such studies be thoroughly familiar with the origin and morphology of normal amniotic fluid cells as well as their growth properties and cytogenetic and biochemical characteristics. The normal variability in each of these parameters must be well defined before the results obtained from a particular sample are assumed to represent fetal abnormality.
KeywordsAmniotic Fluid Prenatal Diagnosis Inborn Error Normal Human Fibroblast Tissue Culture Condition
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