Serum-Factor- and Prolactin-Induced Stimulation of DNA Synthesis by Nuclei Externalized from Mammary Tissue

  • Tomasz Kuryl
  • Lech Zwierzchowski
  • Yale J. Topper
Part of the Biochemical Endocrinology book series (BIOEND)


A very intriguing but poorly understood event in cell biology is the transition from the G1 to the S phase of the cell cycle. It is this event that marks the change from a nonproliferative to a proliferative state. Cell-fusion studies (Rao and Johnson, 1970; Graves, 1972; Rao et al., 1977) suggest that the transition is under positive, rather than negative, control exerted by certain cytoplasmic factors. A variety of exogenous agents, including serum protein factors and hormones, can stimulate cell proliferation in vitro, but the molecular mechanisms have not been delineated.


Horse Serum Native Insulin Mammary Nucleus Relative Incorporation Mammary Epithelial Cell Proliferation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Burton, K., 1956, A study of the conditions and mechanism of diphenylamine reaction forthe colorimetric estimation of deoxyribonucleic acid, Biochem. J. 62: 315.PubMedGoogle Scholar
  2. Chomczynski, P., and Topper, Y. J., 1974, A direct effect of prolactin and placental lactogen on mammary epithelial nuclei, Biochem. Biophys. Res. Commun. 60(1): 56.CrossRefGoogle Scholar
  3. Graves, J. A. M., 1972, DNA synthesis in heterokaryons formed by fusion of mammalians cells from different species, Exp. Cell Res. 72: 393.PubMedCrossRefGoogle Scholar
  4. Hsueh, H. W., and Stockdale, F. E., 1975, Effects of serum on mammary epithelial proliferation in vitro during mammary gland development, J. Cell Biol. 66: 243.PubMedCrossRefGoogle Scholar
  5. Jazwinski, S. M., Wang, J. L., and Edelman, G. M., 1976, Initiation of replication in chromosomal DNA induced by extracts from proliferating cells, Proc. Natl. Acad. Sci. U.S.A. 73:22–31Google Scholar
  6. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., 1951, Protein measurement with Folin phenol reagent, J. Biol. Chem. 193: 265.PubMedGoogle Scholar
  7. Lyon, W. R., Li, C. H., and Johnson, R. E., 1958, The hormonal control of mammary growth and lactation, Recent Prog. Horm. Res. 14: 19.Google Scholar
  8. Majumder, G. C., and Turkington, R. W., 1971, Stimulation of mammary epithelial cell proliferation in vitro by protein factor(s) present in serum, Endocrinology 88: 1506.PubMedCrossRefGoogle Scholar
  9. Nolin, J. M., and Witorsch, R. J., 1976, Detection of endogenous immunoreactive prolactin in rat mammary epithelial cells during lactation, Endocrinology 99: 949.CrossRefGoogle Scholar
  10. Oka, T., and Topper, Y. J., 1972, Is prolactin mitogenic for mammary epithelium?, Proc. Nad. Acad. Sci. U.S.A. 69: 1693.CrossRefGoogle Scholar
  11. Rao, P. N., and Johnson, R. T., 1970, Mammalian cell fusion: Studies on the regulation of DNA synthesis and mitosis, Nature (London) 225: 159.CrossRefGoogle Scholar
  12. Rao, P. N., Sunkara, P. S., and Wilson, B. A., 1977, Regulation of DNA synthesis: Age-dependent cooperation among G1 cells upon fusion, Proc. Natl. Acad. Sci. U.S.A. 74: 2869.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Tomasz Kuryl
    • 1
  • Lech Zwierzchowski
    • 1
  • Yale J. Topper
    • 1
  1. 1.Laboratory of Biochemistry and Metabolism, National Institute of Arthritis, Metabolism, and Digestive DiseasesNational Institutes of HealthBethesdaUSA

Personalised recommendations