Autoradiographic Localization of FSH-Binding Sites on Sertoli Cells and Spermatogonia in Testes from Hypophysectomized Rats
It is now well established that follicle-stimulating hormone (FSH) acts primarily on the seminiferous tubule of the mammalian testis, and that receptors for this action are located on tubule plasma membranes. Specific FSH-binding sites have been demonstrated in isolated seminiferous tubules from immature and mature rats, and it has been shown that most of the binding activity resides in the plasma membrane fraction (Means and Vaitukaitis, 1972; Means, 1973). The biochemical events that are initiated by the binding of FSH to its receptor are also well characterized. Incubation of FSH with either seminiferous tubules (Dorrington et al., 1972) or plasma membranes isolated from tubules (Means, 1975) results in an elevation of cyclic AMP (cAMP) levels that is hormone-and tissue-specific. The cAMP seems to act as an intracellular messenger, causing activation of cAMP-dependent protein kinase and subsequent transcriptional and translational events (Means, 1975).
KeywordsSertoli Cell Leydig Cell Seminiferous Tubule Seminiferous Epithelium Basal Compartment
Abbreviations used in this chapter
electron microscope(-microscopic, microscopy)
light microscope(-microscopic, microscopy)
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