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Autoradiographic Localization of FSH-Binding Sites on Sertoli Cells and Spermatogonia in Testes from Hypophysectomized Rats

  • Joanne Orth
  • A. Kent Christensen
Part of the Biochemical Endocrinology book series (BIOEND)

Abstract

It is now well established that follicle-stimulating hormone (FSH) acts primarily on the seminiferous tubule of the mammalian testis, and that receptors for this action are located on tubule plasma membranes. Specific FSH-binding sites have been demonstrated in isolated seminiferous tubules from immature and mature rats, and it has been shown that most of the binding activity resides in the plasma membrane fraction (Means and Vaitukaitis, 1972; Means, 1973). The biochemical events that are initiated by the binding of FSH to its receptor are also well characterized. Incubation of FSH with either seminiferous tubules (Dorrington et al., 1972) or plasma membranes isolated from tubules (Means, 1975) results in an elevation of cyclic AMP (cAMP) levels that is hormone-and tissue-specific. The cAMP seems to act as an intracellular messenger, causing activation of cAMP-dependent protein kinase and subsequent transcriptional and translational events (Means, 1975).

Keywords

Sertoli Cell Leydig Cell Seminiferous Tubule Seminiferous Epithelium Basal Compartment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used in this chapter

cAMP

cyclic AMP

EM

electron microscope(-microscopic, microscopy)

FSH

follicle-stimulating hormone

hFSH

human FSH

LM

light microscope(-microscopic, microscopy)

SCE

Serto-cell-enriched

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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Joanne Orth
    • 1
  • A. Kent Christensen
    • 1
  1. 1.Department of AnatomyTemple University School of MedicinePhiladelphiaUSA

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