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FSH and the Sertoli Cell

  • Anna Steinberger
  • Barbara M. Sanborn
  • Emil Steinberger
Part of the Biochemical Endocrinology book series (BIOEND)

Abstract

Over a century ago, Sertoli (1865) discovered and described a non-germinal cell in the seminiferous epithelium of the testes. He visualized this cell to be a “supporting” element for the germ cells, a phagocytic element engaged in the removal of germ cell debris and other matter from the seminiferous tubule, and a “nurse” cell for the differentiating germinal elements. This cell was subsequently named the Sertoli cell, and both the morphological description and the suggestions for its function made originally by Sertoli have been supported by either direct or indirect observations.

Keywords

Germ Cell Androgen Receptor Luteinizing Hormone Sertoli Cell Seminiferous Tubule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used in this chapter

ABP

androgen-binding protein

ACTH

adrenocorticotropic hormone

cAMP

cyclic AMP

FSH

follicle-stimulating hormone

LH

luteinizing hormone

MIX

1-methyl-3-isobutyl xanthine

SCE

Sertoli-cell-enriched

SCF

Sertoli-cell factor

TP

testosterone propionate

TSH

thyroid-stimulating hormone

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References

  1. Amann, R. P., and Hammerstedt, R. H., 1976, Binding of steroids by intact bovine sperm, Biol. Reprod. 15: 670.PubMedCrossRefGoogle Scholar
  2. Anderson, J., Clark, J. H., and Peck, E. J., 1972, Oestrogen and nuclear binding sites, Biochem. J. 126: 561.PubMedGoogle Scholar
  3. Baker, H. W. G., Bremner, W. J., Burger, H. G., de Kretser, D. M., Dulmanis, A., Eddie, L. W., Hudson, B., Koegh, E. J., Lee, V. W. K., and Rennie, G. C., 1976, Testicular control of follicle-stimulating hormone secretion, Recent Prog. Horm. Res. 32: 429.PubMedGoogle Scholar
  4. Bardin, C. W., Janne, O., Bullock, L. P., and Jacob, S. T., 1975, Physiochemical and biological properties of androgen receptors, in: Hormonal Regulation of Spermatogenesis ( F. S. French, V. Hansson, E. M. Ritzen, and S. N. Nayfeh, eds.), pp. 237–255, Plenum Press, New York.CrossRefGoogle Scholar
  5. Bass, J. J., Neal, G. E., Bell, J. B. G., and Lacey, D., 1972, Properties of the seminiferous tubules of rat testis consistent with those of androgen dependent tissues, J. Endocrinol. 55:xviii.Google Scholar
  6. Baulieu, E. E., Atger, M., Best-Belpomme, M., Corvol, P., Courvalin, J. C., Mester, J., Milgrom, E., Robel, P., Rochefort, H., and de Catalogne, D., 1975, Steroid hormone receptors, Vitam. Horm. (N. Y.) 33: 649.Google Scholar
  7. Beall, R. J., and Sayers, G., 1972, Isolated adrenal cells: Steroidogenesis and cyclic AMP accumulation in response to ACTH, Arch. Biochem. Biophys. 148: 70.PubMedCrossRefGoogle Scholar
  8. Blaquier, J. A., 1971, Selective uptake and metabolism of androgens by rat epididymis: The presence of a cytoplasmic receptor, Biochem. Biophys. Res. Commun. 45: 1076.PubMedCrossRefGoogle Scholar
  9. Braun, T., and Sepsenwol, S., 1974, Stimulation of 14C-cyclic AMP accumulation by FSH and LH in testis from mature and immature rats, Endocrinology 94: 1028.PubMedCrossRefGoogle Scholar
  10. Buller, R. E., and O’Malley, B. W., 1976, The biology and mechanism of steroid hormone receptor interaction with the eukaryotic nucleus, Biochem. Pharmacol. 25: 1.PubMedCrossRefGoogle Scholar
  11. Burger, H. G., Franchimont, P., de Kretser, D. M., and Hudson, B., 1974, Gonadotropins in spermatogenesis control, in: Recent Progress in Reproductive Endocrinology ( P. G. Crosignani and V. H. James, eds.), pp. 605–631, Academic Press, New York.Google Scholar
  12. Castro, A. E., Seiguer, A. C., and Mancini, R. E., 1970, Electron microscopic study of the localization of labeled gonadotropins in the Sertoli and Leydig cells of the rat testis, Proc. Soc. Exp. Biol. Med. 133: 582.PubMedGoogle Scholar
  13. Castro, A. E., Alonso, A., and Mancini, R. E., 1972, Localization of follicle-stimulating hormone and luteinizing hormone in the rat testis using immunohistological tests, J. Endocrinol. 52: 129.PubMedCrossRefGoogle Scholar
  14. Catt, K. J., and Dufau, M. L., 1973, Interactions of LH and hCG with testicular gonadotropin receptors, Adv. Exp. Med. Biol. 36: 379.PubMedCrossRefGoogle Scholar
  15. Chamness, G. C., Jennings, A. W., and McGuire, W. L., 1974, Estrogen receptor binding to isolated nuclei-A nonsaturable process, Biochemistry 13: 327.PubMedCrossRefGoogle Scholar
  16. Cheng, K. W., 1975, Properties of FSH receptors in cell membranes of bovine testis, Biochem. J. 149: 123.PubMedGoogle Scholar
  17. Chowdhury, M., and Steinberger, E., 1975, Biosynthesis of gonadotrophins by rat pituitaries in vitro, J. Endocrinol. 66: 369.PubMedCrossRefGoogle Scholar
  18. Chowdhury, M., Steinberger, A., and Steinberger, E., 1978, Inhibition of de novo synthesis of FSH by the Sertoli Cell Factor (SCF), Endocrinology (in press).Google Scholar
  19. Desjardins, C., Zeleznik, A. J., Midgley, A. R., Jr., and Reichert, L. E., Jr., 1974, In vitro binding and autoradiographic localization of human chorionic gonadotropin and follicle stimulating hormone in rat testes during development, in: Hormone Binding and Target Cell Activation in the Testis (M. L. Dufau and A. R. Means, ), pp. 221–235, Plenum Press, New York.Google Scholar
  20. Dorrington, J. H., and Armstrong, D. T., 1975, Follicle stimulating hormone stimulates estradiol-17ß synthesis in cultured Sertoli cells, Proc. Natl. Acad. Sci. U.S.A. 72: 2677.PubMedCrossRefGoogle Scholar
  21. Dorrington, J. H., and Fritz, I. B., 1974, Effects of gonadotropins on cyclic AMP production by isolated seminiferous tubule and interstitial cell preparations, Endocrinology 94: 394.CrossRefGoogle Scholar
  22. Dorrington, J. H., Vernon, R. G., and Fritz, I. B., 1972, The effect of gonadotropins on the 3’,5’-AMP levels of seminiferous tubules, Biochem. Biophys. Res. Commun. 46: 1523.PubMedCrossRefGoogle Scholar
  23. Dorrington, J. H., Roller, N. F., and Fritz, I. B., 1974, The effects of FSH on cell preparations from the rat testis, in: Hormone Binding and Target Cell Activation in the Testis ( M. L. Dufau and A. R. Means, eds.), pp. 237–241, Plenum Press, New York.CrossRefGoogle Scholar
  24. Dym, M., and Fawcett, D. W., 1970, Observations on the blood—testis barrier of the rat on the physiological compartmentation of the seminiferous epithelium, Biol. Reprod. 3: 308.PubMedGoogle Scholar
  25. Elkington, J. S. H., 1974, Effects of testosterone propionate (TP) and FSH on levels of testicular high affinity androgen binding protein (ABP) in the rat, 7th Annual Meeting of the Society for the Study of Reproduction, Ottawa, Canada, Abstr. No. 22.Google Scholar
  26. Elkington, J. S. H., Sanborn, B. M., and Steinberger, E., 1975, The effect of testosterone propionate on the concentration of testicular and epididymal androgen binding activity in the hypophysectomized rat, Mol. Cell. Endocrinol. 2: 157.PubMedCrossRefGoogle Scholar
  27. Elkington, J. S. H., Sanborn, B. M., Martin, M. W., Chowdhury, A. K., and Steinberger, E., 1977, Effect of testosterone propionate on ABP levels in rats hypophysectomized at different ages using individual sampling, Mol. Cell. Endocrinol. 6: 203.PubMedCrossRefGoogle Scholar
  28. Exton, J. H., Lewis, S. B., Ho, R. J., Robison, G. A., and Park, C. R., 1971, The role of cyclic AMP in the interaction of glucagon and insulin in the control of liver metabolism, Ann. N. Y. Acad. Sci. 185: 85.PubMedCrossRefGoogle Scholar
  29. Fakunding, J. L., Tindall, D. J., Dedman, J. R., Mena, C. R., and Means, A. R., 1976, Biochemical actions of follicle-stimulating hormone in the Sertoli cell of the rat testis, Endocrinology 98: 392.PubMedCrossRefGoogle Scholar
  30. Fang, S., Anderson, K. M., and Liao, S., 1969, Receptor proteins for androgens: On the role of specific proteins in selective retention of l7ß-hydroxy-5a-androstan-3-one by rat ventral prostate in vivo and in vitro, J. Biol. Chem. 244: 6584.PubMedGoogle Scholar
  31. Fawcett, D. W., 1975, Ultrastructure and function of the Sertoli cell, in: Handbook of Physiology, Section 7, Vol. V ( D. W. Hamilton and R. O. Greep, eds.), pp. 21–55, American Physiological Society, Washington, D. C.Google Scholar
  32. Franchimont, P., 1972, Human gonadotropin secretion, J. R. Coll. Physicians Lond. 6:283. Franchimont, P., Millet, D., Vendrely, E., Legros, J. J., and Netter, A., 1972, Re-lationship between spermatogenesis and serum gonadotropin levels in azoospermia and oligospermia, J. Clin. Endocrinol. Metab. 34: 1003.PubMedCrossRefGoogle Scholar
  33. Franchimont, P., Chari, S., Hagelstein, M. T., and Duraiswami, S., 1975, Existence of a FSH inhibiting factor “inhibin” in bull seminal plasma, Nature (London) 257: 402.CrossRefGoogle Scholar
  34. Franchimont, P., Chari, S., Hazee-Hagelstein, M. T., Debruche, M. L., and Duraiswami, S., 1977, Evidence for the existence of inhibin, in: The Testis in Normal and Infertile Men ( P. Troen and H. R. Nankin, eds.), pp. 253–270, Raven Press, New York.Google Scholar
  35. French, F. S., and Ritzen, E. M., 1973a, A high-affinity androgen binding protein (ABP) in rat testis: Evidence for secretion into efferent duct fluid and absorption by epididymis, Endocrinology 93: 88.PubMedCrossRefGoogle Scholar
  36. French, F. S., and Ritzen, E. M., 1973b, Androgen-binding protein in efferent duct fluid of rat testis, J. Reprod. Fertil. 32: 479.PubMedCrossRefGoogle Scholar
  37. Fritz, I. B., Kopec, B., Lam, K., and Vernon, R. G., 1974, Effects of FSH on levels of androgen binding protein in the testis, in: Hormone Binding and Target Cell Activation in the Testis ( M. L. Dufau and A. R. Means, eds.), pp. 311–327, Plenum Press, New York.CrossRefGoogle Scholar
  38. Fritz, I. B., Louis, B. G., Tung, P. S., Griswold, M., Rommerts, F. G., and Dorrington, J. H., 1975, Biochemical responses of cultured Sertoli cell enriched preparations to follicle stimulating hormone and dibutyryl cyclic AMP in: Hormonal Regulation of Spermatogenesis (F. S. French, V. Hansson, E. M. Ritzen, and S. N. Nayfeh, ), pp. 367–382, Plenum Press, New York.Google Scholar
  39. Galena, H. J., Pillai, A. K., and Terner, C., 1974, Progesterone and androgen receptors in non-flagellate germ cells of the rat testis, J. Endocrinol. 63: 223.PubMedCrossRefGoogle Scholar
  40. Hagenas, L., Ritzen, E. M., Ploen, L., Hansson, V., French, F. S., and Nayfeh, S. N., 1975, Sertoli cell origin of testicular androgen binding protein (ABP), Mol. Cell. Endocrinol. 4: 339.CrossRefGoogle Scholar
  41. Hansson, V., and Tveter, K. J., 1971, Uptake and binding in vivo of 3H labeled androgen in the rat epididymis and ductus deferens, Acta Endocrinol. (Copenhagen) 66: 745.Google Scholar
  42. Hansson, V., Djpseland, O., Reusch, E., Attramadal, A., and Torgersen, O., 1973a, An androgen binding protein in the testis cytosol fraction of adult rats: Comparison with the androgen binding protein in the epididymis, Steroids 21: 457.PubMedCrossRefGoogle Scholar
  43. Hansson, V., Djgseland, O., Reusch, E., Attramadal, A., and Torgersen, O., 1973b, Intracellular receptors for 5a-dihydrotestosterone in the epididymis of adult rats: Comparison with the androgenic receptors in the ventral prostate and the androgen binding protein (ABP) in the testicular and epididymal fluid, Steroids 22: 19.PubMedCrossRefGoogle Scholar
  44. Hansson, V., Reusch, E., Trygstad, O., Torgersen, O., Ritzen, E. M., and French, F. S., 1973c, FSH stimulation of testicular androgen binding protein, Nature (London) New Biol. 246: 56.Google Scholar
  45. Hansson, V., McLean, W. S., Smith, A. A., Tindall, D. J., Weddington, S. C., Nayfeh, S. N., and French, F. S., 1974, Androgen receptors in rat testis, Steroids 23: 823.PubMedGoogle Scholar
  46. Hansson, V., Ritzen, E. M., French, F. S., and Nayfeh, S. N., 1975a, Androgen transport and receptor mechanisms in testis and epididymis, in: Handbook of Physiology ( D. W. Hamilton and R. O. Greep, eds.), pp. 173–201, American Physiological Society, Washington, D.C.Google Scholar
  47. Hansson, V., Weddington, S. C., Naess, O., Attramadal, A., French, F. S., Kotite, N., Nayfeh, S. N., Ritzen, E. M., and Hagenas, L., 1975b, Testicular androgen binding protein (ABP)—A parameter of Sertoli cell secretory function, in: Hormonal Regulation of Spermatogenesis ( F. S. French, V. Hansson, E. M. Ritzen, and S. N. Nayfeh, eds.), pp. 323–336, Plenum Press, New York.CrossRefGoogle Scholar
  48. Heindel, J. J., Rothenberg, R., Robison, G. A., and Steinberger, A., 1975, LH and FSH stimulation of cyclic AMP in specific cell types isolated from the testes, J. Cyclic Nucleotide Res. 1: 69.PubMedGoogle Scholar
  49. Heindel, J. J., Hintz, M. I., Steinberger, E., and Strada, S. J., 1977, Effects of FSH on cy-clic nucleotide levels in testis of rats of various age, Endocr. Res. Commun. 4:311. Imai, K., 1970, Binding proteins of androgen in the testis of the rat, Gunma Symp. En-docrinol. 7:169.Google Scholar
  50. Keogh, E. J., Lee, V. W. K., Rennie, G. C., Burger, H. G., Hudson, B., and de Kretser, D. M., 1976, Selective suppression of FSH by testicular extracts, Endocrinology 98: 997.PubMedCrossRefGoogle Scholar
  51. Klyzsejko-Stefanowicz, L., Chiu, J. F., Tsai, Y. H., and Hnilica, L., 1976, Acceptor pro-teins in rat androgenic tissue chromatin, Proc. Natl. Acad. Sci. U.S.A. 73: 1954.PubMedCrossRefGoogle Scholar
  52. Krueger, P. M., Hodgen, G. D., and Sherins, R. J., 1974, New evidence for the role of the Sertoli cell and spermatogonia in feedback control of FSH secretion in male rats, En-docrinology 95:955.Google Scholar
  53. Kuehl, F. A., Jr., Patanelli, D. J., Tarnoff, J., and Humes, J. L., 1970, Testicular adenyl cyclase: Stimulation by the pituitary gonadotrophins, Biol. Reprod. 2: 154.PubMedCrossRefGoogle Scholar
  54. Liao, S., 1975, Cellular receptors and mechanisms of action of steroid hormones, Int. Rev.Cytol. 41: 87.PubMedCrossRefGoogle Scholar
  55. Louis, B. G., and Fritz, I. B., 1977, Stimulation by androgens of the production of an-drogen binding protein by cultured Sertoli cells, Mol. Cell. Endocrinol. 7: 9.PubMedCrossRefGoogle Scholar
  56. Louis, B. G., Griswold, M. D., and Fritz, I. B., 1975, Stimulation of androgen binding pro-tein production by enriched Sertoli cell preparations in culture by FSH and cyclic AMP, 57th Annual Meeting of The Endocrine Society, New York, Abstr. No. 253.Google Scholar
  57. Lugaro, G., Casellato, M. M., Mazzola, G., Fachini, G., and Carrea, G., 1974, Evidence for the existence in spermatozoa of a factor inhibiting the follicle stimulating hormone releasing hormone synthesis, Neuroendocrinology 15: 62.CrossRefGoogle Scholar
  58. Lyons, M. F., Glenister, P. H., and Lamoreux, M. L., 1975, Normal spermatozoa from androgen-resistant germ cells of chimaeric mice and the role of androgen in spermatogenesis, Nature (London) 258: 620.CrossRefGoogle Scholar
  59. Mancini, R. E., Castro, A., and Seiguer, A. C., 1967, Histological localization of follicle-stimulating and luteinizing hormones in the rat testis, J. Histochem. Cytochem. 15:516. McCullagh, D. R., 1932, Dual endocrine activity of testes, Science 76: 19.Google Scholar
  60. McLean, W. S., Smith, A. A., Hansson, V., Naess, O., Nayfeh, S. N., and French, F. S., 1976, Further characterization of androgen receptor in rat testis, Mol. Cell. Endocrinol. 4: 239.PubMedCrossRefGoogle Scholar
  61. Means, A. R., 1974, Early sequence of biochemical events in the action of follicle stimulating hormone on the testis, Life Sci. 15: 371.PubMedCrossRefGoogle Scholar
  62. Means, A. R., and Hall, P. F., 1967, Effect of FSH on protein biosynthesis in testes of the immature rat, Endocrinology 81: 1151.PubMedCrossRefGoogle Scholar
  63. Means, A. R., and Hall, P. F., 1968, Protein biosynthesis in the testis. I. Comparison between stimulation by FSH and glucose, Endocrinology 82: 597.PubMedCrossRefGoogle Scholar
  64. Means, A. R., and Vaitukaitis, J., 1972, Peptide hormone “receptors”: Specific binding of 3H-FSH to testis, Endocrinology 90: 39.PubMedCrossRefGoogle Scholar
  65. Means, A. R., Fakunding, J. L., Huckins, C., Tindall, D. J., and Vitale, R., 1976, Follicle-stimulating hormone, the Sertoli cell, and spermatogenesis, Recent Prog. Horm. Res. 32: 477.PubMedGoogle Scholar
  66. Monn, E., Desautel, M., and Christiansen, R. 0., 1972, Highly specific testicular adenosine 3’,5’-monophosphate phosphodiesterase associated with sexual maturation, Endocrinology 91: 716.Google Scholar
  67. Mulder, E., Peters, M. J., van Beurden, W. M. O., and van der Molen, H. J., 1974, A receptor for testosterone in mature rat testes, FEBS Lett. 47: 20.CrossRefGoogle Scholar
  68. Mulder, E., Peters, M. J., de Vries, J., and van der Molen, H. J., 1975, Characterization of a nuclear receptor for testosterone in seminiferous tubules of mature rat testes, Mol. Cell. Endocrinol. 2: 171.PubMedCrossRefGoogle Scholar
  69. Mulder, E., Peters, M. J., van Beurden, W. M. O., Galdieri, M., Rommerts, F. F. G., Janszen, F. H. A., and van der Molen, H. J., 1976, Androgen receptors in isolated cell preparations obtained from rat testicular tissue, J. Endocrinol. 70: 331.PubMedCrossRefGoogle Scholar
  70. Murad, F., Strauch, B., and Vaughan, M., 1969, The effect of gonadotropins on testicular adenyl cyclase, Biochim. Biophys. Acta 177: 591.PubMedCrossRefGoogle Scholar
  71. Murphy, H. D., 1965, Sertoli cell stimulation following intratesticular injections of FSH in the hypophysectomized rat, Proc. Soc. Exp. Biol. Med. 118: 1202.PubMedGoogle Scholar
  72. Nandini, S. G., Lipnez, H., and Moudgal, N. R., 1976, A model system for studying inhibin, Endocrinology 98: 1460.PubMedCrossRefGoogle Scholar
  73. Orth, J., and Christensen, A. K., 1977, Localization of 125I-FSH in the testes of hypophysectomozed rats by autoradiography at the light and electron microscope levels, Endocrinology 101: 262.PubMedCrossRefGoogle Scholar
  74. Paulsen, C. A., Leonard, J. M., de Kretser, D. M., and Leach, R. B., 1972, Interrelationship between spermatogenesis and follicle-stimulating hormone levels, in: Gonadotropins ( B. B. Saxena, C. G. Beling, and H. M. Gandy, eds.), pp. 628–639, Wiley-Interscience, New York.Google Scholar
  75. Reichert, L. E., and Bhalla, V. K., 1974, Development of a radioligand tissue receptor assay for human follicle stimulating hormone, Endocrinology 94: 483.PubMedCrossRefGoogle Scholar
  76. Ritzen, E. M., Nayfeh, S. N., French, F. S., and Dobbins, M. C., 1971, Demonstration of androgen-binding components in rat epididymis cytosol and comparison with binding components in prostate and other tissues, Endocrinology 89: 143.PubMedCrossRefGoogle Scholar
  77. Ritzen, E. M., Dobbins, M. C., Tindall, D. J., French, F. S., and Nayfeh, S. N., 1973, Characterization of an androgen binding protein (ABP) in rat testis and epididymis, Steroids 21: 593.PubMedCrossRefGoogle Scholar
  78. Ritzen, E. M., Hagenas, L., Hansson, V., and French, F. S., 1975, In vitro synthesis of testicular androgen binding protein (ABP): Stimulation by FSH and androgen, in: Hormonal Regulation of Spermatogenesis (F. S. French, V. Hansson, E. M. Ritzen, and S. N. Nayfeh, ), pp. 353–366, Plenum Press, New York.Google Scholar
  79. Sanborn, B. M., and Steinberger, E., 1975, Androgen nuclear exchange activity in rat testis, Endocr. Res. Commun. 2: 335.PubMedCrossRefGoogle Scholar
  80. Sanborn, B. M., Elkington, J. S. H., and Steinberger, E., 1974a, Properties of rat testicular androgen binding proteins, in: Hormone Binding and Target Cell Activation in the Testis ( M. L. Dufau and A. R. Means, eds.), pp. 291–310, Plenum Press, New York.CrossRefGoogle Scholar
  81. Sanborn, B. M., Elkington, J. S. H., and Steinberger, E., 1974b, Hormonal influences on the testicular level of androgen binding protein, 56th Annual Meeting of The Endocrine Society, Atlanta, Georgia, Abstr. No. 288.Google Scholar
  82. Sanborn, B. M., Elkington, J. S. H., Steinberger, A., Steinberger, E., and Meistrich, M. L., 1975a, Androgen binding in the testis: In vitro production of androgen binding protein by Sertoli cell cultures and measurement of nuclear binding by a nuclear exchange assay, in: Hormonal Regulation of Spermatogenesis ( F. S. French, V. Hans-son, and E. M. Ritzen, eds.), pp. 293–310, Plenum Press, New York.CrossRefGoogle Scholar
  83. Sanborn, B. M., Elkington, J. S. H., Chowdhury, M., Tcholakian, R. K., and Steinberger,E., 1975b, Hormonal influences on the level of testicular androgen binding activity: Effect of FSH following hypophysectomy, Endocrinology 96: 326.CrossRefGoogle Scholar
  84. Sanborn, B. M., Elkington, J. S. H., Tcholakian, R. K., and Steinberger, E., 1975c, Some properties of androgen-binding activity in rat testis, Mol. Cell. Endocrinol. 3: 129.PubMedCrossRefGoogle Scholar
  85. Sanborn, B. M., Steinberger, A., Meistrich, M. L., and Steinberger, E., 1975d, Androgen binding sites in testis cell fractions as measured by a nuclear exchange assay, J. Ster.Biochem. 6: 1459.CrossRefGoogle Scholar
  86. Sanborn, B. M., Elkington, J. S. H., Steinberger, A., Steinberger, E., and Meistrich, M. L., 1976a, Androphdic proteins in the testis, in: Regulatory Mechanisms of Male Reproductive Physiology ( C. H. Spilman, T. J. Lobl., and K. T. Kirton, eds.), pp. 45–58, Excerpta Medica, Amsterdam.Google Scholar
  87. Sanborn, B. M., Steinberger, A., and Steinberger, E., 1976b, Nuclear-bound androgen in cultured Sertoli cells and germ cells from rat testes, 58th Annual Meeting of the Endocrine Society, San Francisco, Abstr. No. 77.Google Scholar
  88. Sanborn, B. M., Steinberger, A., Tcholakian, R. K., and Steinberger, E., 1977, Direct measurement of androgen receptors in cultured Sertoli cells, Steroids 29: 493.PubMedCrossRefGoogle Scholar
  89. Sanborn, B. M., Tsai, Y. H., Steinberger, A., and Steinberger, E., 1978, Biochemical as-pects of the interaction of androgens with Sertoli cells, Ann. Biol. Anim. Biochim. Bio-phys. 18: 615.CrossRefGoogle Scholar
  90. Schwartz, N. B., and Channing, C. P., 1977, Suppression by porcine follicular fluid of the acute serum FSH rise following ovariectomy in the rat, 10th Annual Meeting of the Society for the Study of Reproduction, Austin, Texas, Abstr. No. 37.Google Scholar
  91. Sertoli, E., 1865, Dell’esistenzia di particolari cellule ramificati nei canalicoli seminiferi del testicolo umano, Morgagni 7:31.Google Scholar
  92. Setchell, B. P., 1974, Secretions of the testis and epididymis, J. Reprod. Fertil. 37:165. Setchell, B. P., and Jacks, F., 1974, Inhibin-like activity in rete testis fluid, J. Endocrinol. 62: 675.PubMedCrossRefGoogle Scholar
  93. Setchell, B. P., and Sirinathsinghji, D. J., 1972, Antigonadotrophic activity in rete testis fluid, a possible “inhibin,” J. Endocrinol. 53:lx.Google Scholar
  94. Sherwood, O. D., Grimek, H. J., and McShan, W. H., 1970, Purification and properties of follicle-stimulating hormones from sheep pituitary glands, J. Biol. Chem. 245: 2328.PubMedGoogle Scholar
  95. Steinberger, A., 1976, Selective inhibition of FSH secretion by Sertoli cell cultures in vitro, 58th Annual Meeting of The Endocrine Society, San Francisco, Abstr. No. 363.Google Scholar
  96. Steinberger, A., and Heindel, J. J., 1977, FSH-stimulation of cyclic AMP in isolated Sertoli cells from rats of different ages, 2nd Annual Meeting of the American Society of Andrology, Palm Springs, California, Abstr. No. 57.Google Scholar
  97. Steinberger, A., and Steinberger, E., 1971, Replication pattern of Sertoli cells in maturing rat testis in vivo and in organ culture, Biol. Reprod. 4: 84.PubMedGoogle Scholar
  98. Steinberger, A., and Steinberger, E., 1976a, Secretion of an FSH-inhibiting factor by cultured Sertoli cells, Endocrinology 99: 918.PubMedCrossRefGoogle Scholar
  99. Steinberger, A., and Steinberger, E., 1976b, Action of gonadotropins in testes organ and cell culture, in: Sperm Action, Progress in Reproductive Biology, Vol. I ( P. O. Hubinont, ed.), pp. 42–54, S. Karger, Basel.Google Scholar
  100. Steinberger, A., and Steinberger, E., 1977, Inhibition of FSH by a Sertoli cell-factor (SCF) in vitro, in: The Testis in Normal and Infertile Men ( P. Troen and H. R. Nankin, eds.), pp. 271–279, Raven Press, New York.Google Scholar
  101. Steinberger, A., and Walther, J., 1977, Age-dependent responses of isolated Sertoli cells to FSH, 10th Annual Meeting of the Society for the Study of Reproduction, Austin, Texas, Abstr. No. 26.Google Scholar
  102. Steinberger, A., Yang, K. P., and Ward, D. N., 1973, Recovery of LH binding activity in cultures of enzymatically isolated interstitial cells, 55th Annual Meeting of The Endocrine Society, Chicago, Abstr. No. 447.Google Scholar
  103. Steinberger, A., Thanki, K. J., and Siegal, B., 1974, FSH binding in rat testes during maturation and following hypophysectomy: Cellular localization of FSH receptors, in: Hormone Binding and Target Cell Activation in the Testis ( M. L. Dufau and A. R. Means, eds.), pp. 177–191, Plenum Press, New York.CrossRefGoogle Scholar
  104. Steinberger, A., Heindel, J. J., Lindsey, J. N., Elkington, J. S. H., Sanborn, B. M., and Steinberger, E., 1975a, Isolation and culture of FSH responsive Sertoli cells, Endocr. Res. Commun. 2: 261.PubMedCrossRefGoogle Scholar
  105. Steinberger, A., Heindel, J. J., Lindsey, J. N., Elkington, J. S. H., Sanborn, B. M., and Steinberger, E., 1975b, Culture and FSH responses of Sertoli cells isolated from sexually mature rat testis, in: Hormonal Regulation of Spermatogenesis ( F. S. French, V. Hansson, E. M. Ritzen, and S. N. Nayfeh, eds.), pp. 399–411, Plenum Press, New York.CrossRefGoogle Scholar
  106. Steinberger, A., Hintz, M., and Heindel, J. J., 1978, Changes in cyclic AMP responses toFSH in isolated rat Sertoli cells during sexual maturation, Biol. Reprod. (in press).Google Scholar
  107. Steinberger, E., 1971, Hormonal control of mammalian spermatogenesis, Physiol. Rev. 51: 1.Google Scholar
  108. Steinberger, E., 1975, Hormonal regulation of the seminiferous tubule function, in: Hormonal Regulation of Spermatogenesis ( F. S. French, V. Hansson, E. M. Ritzen, and S. N. Nayfeh, eds.), pp. 337–352, Plenum Press, New York.CrossRefGoogle Scholar
  109. Steinberger, E., and Chowdhury, M., 1974, Control of pituitary FSH in male rats, Acta Endocrinol. (Copenhagen) 76: 235.Google Scholar
  110. Steinberger, E., and Duckett, G. E., 1966, Pituitary “total” gonadotropins, FSH and LH in orchiectomized or cryptorchid rats, Endocrinology 79: 912.PubMedCrossRefGoogle Scholar
  111. Steinberger, E., and Duckett, G. E., 1967, Hormonal control of spermatogenesis, J. Re-prod. Fertil. 2: 75.Google Scholar
  112. Steinberger, E., Steinberger, A., and Perloff, W. H., 1964, Initiation of spermatogenesis in vitro, Endocrinology 74: 788.Google Scholar
  113. Steinberger, E., Steinberger, A., and Sanborn, B., 1974, Endocrine control of spermatogenesis, in: Physiology and Genetics of Reproduction, Part A ( M. Coutinho and F. Fuchs, eds.), pp. 163–181, Plenum Press, New York.Google Scholar
  114. Steinberger, E., Steinberger, A., and Sanborn, B. M., 1978, Molecular mechanisms concerned with hormonal control of the seminiferous epithelium, in: Recent Progress in Andrology, Serono Symposium (A. Fabbrini and E. Steinberger), Academic Press, New York (in press).Google Scholar
  115. Swerdloff, R. S., Walsh, P. C., Jacobs, H. S., and Odell, W. D., 1971, Serum LH and FSH during sexual maturation in the male rat: Effect of castration and cryptorchidism, Endocrinology 88: 120.PubMedCrossRefGoogle Scholar
  116. Thanki, K. H., and Steinberger, A., 1978, Effect of age and hypophysectomy on FSH binding in rat testes, Andrologia 10: 195.PubMedCrossRefGoogle Scholar
  117. Tindall, D. J., Schrader, W. T., and Means, A R., 1974, The production of androgen binding protein by Sertoli cells, in: Hormone Binding and Target Cell Activation in the Testis ( M. L. Dufau and A. R. Means, eds.) pp. 167–176, Plenum Press, New York.CrossRefGoogle Scholar
  118. Tindall, D. J., Miller, D. A., and Means, A. R., 1977, Characterization of androgen receptor in Sertoli cell-enriched testis, Endocrinology 101: 13.PubMedCrossRefGoogle Scholar
  119. Tsai, Y. H., Sanborn, B. M., Steinberger, A., and Steinberger, E., 1977a, Interaction of testicular androgen—receptor complex with rat germ cell and Sertoli cell chromatin, Biochem. Biophys. Res. Commun. 25: 366.CrossRefGoogle Scholar
  120. Tsai, Y. H.,, Sanborn, B. M., Steinberger, A., and Steinberger, E., 1977b, The effect of ionic strength on the interaction of testicular androgen—receptor complex with Sertoli cell chromatin, 17th Annual Meeting of the American Society for Cell Biology Meeting, San Diego, Abstr. No. 591.Google Scholar
  121. Van Sickle, M., Oberwetter, J., Birnbaumer, L., and Means, A., 1977, Developmental changes and characterization of adenylyl cyclase in the testis Sertoli cell, 10th Annual Meeting of the Society for the Study of Reproduction, Austin, Texas, Abstr. No. 27.Google Scholar
  122. van Thiel, D. H., Sherins, R. J., Myers, G. H., Jr., and De Vita, V. T., Jr., 1972, Evidence for a specific seminiferous tubular factor affecting follicle-stimulating hormone secretion in man, J. Clin. Invest. 51: 1009.PubMedCrossRefGoogle Scholar
  123. Verhoeven, G., Heyns, W., and DeMoor, P., 1975, Ammonium sulfate precipitation as a tool for the study of androgen receptor proteins in rat prostate and mouse kidney, Steroids 26: 149.PubMedCrossRefGoogle Scholar
  124. Vernon, R. G., Kopec, B., and Fritz, I. B., 1974, Observations on the binding of androgens by rat testis seminiferous tubules and testis extracts, Mol. Cell. Endocrinol. 1: 167.CrossRefGoogle Scholar
  125. Vilar, O., Steinberger, A., and Steinberger, E., 1967, An electronic microscopic study of cultured rat testicular fragments, Z. Zellforsch. 78: 221.PubMedCrossRefGoogle Scholar
  126. Weddington, S. C., Hansson, V., Ritzen, E. M., Hagenas, L., French, F. S., and Nayfeh, S. N., 1975, Sertoli cell secretory function after hypophysectomy, Nature (London) 254: 145.CrossRefGoogle Scholar
  127. Welsh, M. J., and Wiebe, J. P., 1976, Sertoli cells from immature rats: In vitro stimulation of steroid metabolism by FSH, Biochem. Biophys. Res. Commun. 69: 936.PubMedCrossRefGoogle Scholar
  128. Williams, J. A., 1972, Cyclic AMP formation and thyroid secretion by incubated mouse thyroid lobes, Endocrinology 91: 1411.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Anna Steinberger
    • 1
  • Barbara M. Sanborn
    • 1
  • Emil Steinberger
    • 1
  1. 1.Department of Reproductive Medicine and BiologyThe University of Texas Health Science Center at Houston, Medical SchoolHoustonUSA

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